GGA3 (NM_138619) Human Tagged ORF Clone

CAT#: RC213361

GGA3 (Myc-DDK-tagged)-Human golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA3), transcript variant long

Product Images

Western blot validation of overexpression lysate (Cat# LY408454) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213361 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GGA3 protein (Cat# TP313361). The protein was produced from HEK293T cells transfected with GGA3 cDNA clone (Cat# RC213361) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GGA3
• Synonyms: KIAA0154
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213361 representing NM_138619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGGCGGAAGGGGAAAGCCTGGAGTCCTGGCTCAATAAAGCCACCAATCCTTCCAACCGCCAGG
  AGGACTGGGAATACATAATTGGCTTCTGTGATCAGATCAACAAGGAGCTGGAAGGGCCACAGATCGCCGT
  CCGACTGCTGGCCCACAAGATCCAGTCCCCACAGGAATGGGAGGCGCTCCAGGCCCTGACGGTGCTGGAG
  GCATGCATGAAGAACTGTGGGAGGAGATTTCATAACGAAGTGGGGAAGTTCCGCTTTTTGAATGAGTTAA
  TCAAAGTCGTCTCTCCAAAGTACCTGGGGGACAGGGTGTCTGAGAAAGTGAAGACCAAGGTTATTGAGCT
  GCTGTACAGCTGGACCATGGCCCTGCCAGAAGAAGCAAAGATCAAAGACGCCTACCACATGCTGAAGAGA
  CAGGGCATAGTGCAGTCTGACCCACCAATTCCTGTGGATAGGACGCTGATCCCCTCTCCACCACCTCGTC
  CCAAAAACCCTGTTTTTGATGATGAGGAGAAGTCCAAGCTTTTAGCCAAGCTGCTGAAAAGCAAAAACCC
  AGATGACCTGCAGGAGGCCAACAAGCTCATCAAGTCCATGGTGAAGGAAGACGAGGCACGGATCCAGAAG
  GTGACCAAGCGTCTGCACACGTTAGAGGAAGTTAACAACAACGTGAGACTGCTCAGTGAGATGCTGCTTC
  ATTACAGCCAGGAGGACTCTTCGGACGGGGACAGAGAGCTGATGAAGGAGCTGTTTGATCAGTGTGAGAA
  CAAGAGGCGGACTTTATTTAAACTCGCCAGTGAGACTGAGGACAATGATAACAGTTTGGGGGACATCCTG
  CAAGCCAGTGACAACCTCTCCCGGGTCATCAACTCTTACAAAACAATTATTGAAGGGCAGGTCATCAATG
  GCGAGGTGGCTACCTTAACCCTGCCTGACTCGGAAGGAAACAGTCAGTGCAGTAACCAAGGCACGCTCAT
  CGACCTTGCGGAGCTGGACACGACCAACAGTTTGTCCTCCGTGTTGGCCCCAGCACCTACTCCACCCTCC
  TCAGGCATCCCAATCCTCCCTCCACCACCCCAGGCCTCAGGACCTCCACGGAGCCGCTCCTCTAGCCAGG
  CCGAGGCCACCCTGGGGCCCAGCAGCACAAGCAACGCCCTCTCCTGGCTGGACGAGGAGCTACTCTGCTT
  GGGCCTCGCCGACCCAGCCCCTAATGTTCCTCCCAAAGAGTCAGCTGGGAACAGCCAGTGGCACCTGCTC
  CAGAGGGAACAGTCCGACCTGGACTTCTTCAGCCCCAGGCCGGGGACCGCTGCCTGTGGCGCCTCCGATG
  CTCCTCTGCTCCAGCCCTCAGCCCCCTCCTCAAGCAGCTCCCAAGCTCCACTGCCGCCTCCCTTCCCAGC
  TCCTGTGGTCCCAGCCAGTGTTCCTGCCCCCAGTGCGGGCTCCTCCTTGTTTTCTACTGGAGTGGCCCCA
  GCCTTGGCCCCAAAAGTTGAGCCCGCAGTCCCTGGGCACCATGGCTTGGCGTTGGGCAACAGCGCGCTGC
  ACCACCTGGATGCCCTCGATCAGCTTCTAGAAGAGGCCAAAGTGACCTCGGGCTTGGTGAAACCCACTAC
  CTCCCCTCTCATCCCCACCACCACCCCAGCCAGGCCCCTCCTGCCCTTCTCCACGGGGCCCGGCAGCCCG
  CTCTTCCAGCCACTGAGTTTCCAGTCCCAGGGCAGCCCCCCGAAGGGGCCTGAGCTCTCCCTGGCCAGCA
  TCCACGTGCCCCTGGAATCGATCAAGCCTAGCAGTGCCCTTCCTGTGACAGCCTACGATAAAAACGGCTT
  CCGCATCCTCTTCCACTTTGCCAAGGAGTGTCCCCCAGGACGACCTGACGTGCTGGTGGTGGTGGTGTCC
  ATGCTGAACACGGCTCCCTTACCTGTCAAGAGCATCGTGCTGCAGGCTGCAGTGCCCAAGTCAATGAAAG
  TGAAGTTGCAGCCACCTTCTGGGACAGAACTCTCTCCATTTAGCCCCATCCAGCCACCTGCAGCCATCAC
  CCAGGTCATGTTGCTGGCCAATCCACTGAAGGAGAAGGTGCGGCTTCGGTATAAGCTGACCTTCGCCCTG
  GGGGAGCAGCTGAGCACAGAGGTGGGCGAGGTGGACCAGTTCCCTCCTGTGGAACAGTGGGGGAACCTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213361 representing NM_138619
  Red=Cloning site Green=Tags(s)
  
  MAEAEGESLESWLNKATNPSNRQEDWEYIIGFCDQINKELEGPQIAVRLLAHKIQSPQEWEALQALTVLE
  ACMKNCGRRFHNEVGKFRFLNELIKVVSPKYLGDRVSEKVKTKVIELLYSWTMALPEEAKIKDAYHMLKR
  QGIVQSDPPIPVDRTLIPSPPPRPKNPVFDDEEKSKLLAKLLKSKNPDDLQEANKLIKSMVKEDEARIQK
  VTKRLHTLEEVNNNVRLLSEMLLHYSQEDSSDGDRELMKELFDQCENKRRTLFKLASETEDNDNSLGDIL
  QASDNLSRVINSYKTIIEGQVINGEVATLTLPDSEGNSQCSNQGTLIDLAELDTTNSLSSVLAPAPTPPS
  SGIPILPPPPQASGPPRSRSSSQAEATLGPSSTSNALSWLDEELLCLGLADPAPNVPPKESAGNSQWHLL
  QREQSDLDFFSPRPGTAACGASDAPLLQPSAPSSSSSQAPLPPPFPAPVVPASVPAPSAGSSLFSTGVAP
  ALAPKVEPAVPGHHGLALGNSALHHLDALDQLLEEAKVTSGLVKPTTSPLIPTTTPARPLLPFSTGPGSP
  LFQPLSFQSQGSPPKGPELSLASIHVPLESIKPSSALPVTAYDKNGFRILFHFAKECPPGRPDVLVVVVS
  MLNTAPLPVKSIVLQAAVPKSMKVKLQPPSGTELSPFSPIQPPAAITQVMLLANPLKEKVRLRYKLTFAL
  GEQLSTEVGEVDQFPPVEQWGNL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138619
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_138619.1, NM_138619.2, NM_138619.3, NP_619525.1
• RefSeq Size: 3860
• RefSeq ORF: 2172
• Locus ID: 23163
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome
• MW: 78.1 kDa
• Gene Summary: This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]