TRPM1 (NM_002420) Human Tagged ORF Clone

CAT#: RC213443

TRPM1 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 1 (TRPM1)

Product Images

Western blot validation of overexpression lysate (Cat# LY419339) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213443 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRPM1
• Synonyms: CSNB1C; LTRPC1; MLSN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213443 representing NM_002420
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGACTCTAACAGGTGTTGCTGTGGCCAGTTCACCAACCAGCATATCCCCCCTCTGCCAAGTGCAA
  CACCCAGCAAAAATGAAGAGGAAAGCAAACAGGTGGAGACTCAGCCTGAGAAATGGTCTGTTGCCAAGCA
  CACCCAGAGCTACCCAACAGATTCCTATGGAGTTCTTGAATTCCAGGGTGGCGGATATTCCAATAAAGCC
  ATGTATATCCGTGTATCCTATGACACCAAGCCAGACTCACTGCTCCATCTCATGGTGAAAGATTGGCAGC
  TGGAACTCCCCAAGCTCTTAATATCTGTGCATGGAGGCCTCCAGAACTTTGAGATGCAGCCCAAGCTGAA
  ACAAGTCTTTGGGAAAGGCCTGATCAAGGCTGCTATGACCACCGGGGCCTGGATCTTCACCGGGGGTGTC
  AGCACAGGTGTTATCAGCCACGTAGGGGATGCCTTGAAAGACCACTCCTCCAAGTCCAGAGGCCGGGTTT
  GTGCTATAGGAATTGCTCCATGGGGCATCGTGGAGAATAAGGAAGACCTGGTTGGAAAGGATGTAACAAG
  AGTGTACCAGACCATGTCCAACCCTCTAAGTAAGCTCTCTGTGCTCAACAACTCCCACACCCACTTCATC
  CTGGCTGACAATGGCACCCTGGGCAAGTATGGCGCCGAGGTGAAGCTGCGAAGGCTGCTGGAAAAGCACA
  TCTCCCTGCAGAAGATCAACACAAGACTGGGGCAGGGCGTGCCCCTCGTGGGTCTCGTGGTGGAGGGGGG
  CCCTAACGTGGTGTCCATCGTCTTGGAATACCTGCAAGAAGAGCCTCCCATCCCTGTGGTGATTTGTGAT
  GGCAGCGGACGTGCCTCGGACATCCTGTCCTTTGCGCACAAGTACTGTGAAGAAGGCGGAATAATAAATG
  AGTCCCTCAGGGAGCAGCTTCTAGTTACCATTCAGAAAACATTTAATTATAATAAGGCACAATCACATCA
  GCTGTTTGCAATTATAATGGAGTGCATGAAGAAGAAAGAACTCGTCACTGTGTTCAGAATGGGTTCTGAG
  GGCCAGCAGGACATCGAGATGGCAATTTTAACTGCCCTGCTGAAAGGAACAAACGTATCTGCTCCAGATC
  AGCTGAGCTTGGCACTGGCTTGGAACCGCGTGGACATAGCACGAAGCCAGATCTTTGTCTTTGGGCCCCA
  CTGGCCGCCCCTGGGAAGCCTGGCACCCCCGACGGACAGCAAAGCCACGGAGAAGGAGAAGAAGCCACCC
  ATGGCCACCACCAAGGGAGGAAGAGGAAAAGGGAAAGGCAAGAAGAAAGGGAAAGTGAAAGAGGAAGTGG
  AGGAAGAAACTGACCCCCGGAAGATAGAGCTGCTGAACTGGGTGAATGCTTTGGAGCAAGCGATGCTAGA
  TGCTTTAGTCTTAGATCGTGTCGACTTTGTGAAGCTCCTGATTGAAAACGGAGTGAACATGCAACACTTT
  CTGACCATTCCGAGGCTGGAGGAGCTTTATAACACAAGACTGGGTCCACCAAACACACTTCATCTGCTGG
  TGAGGGATGTGAAAAAGAGCAACCTTCCGCCTGATTACCACATCAGCCTCATAGACATCGGGCTCGTGCT
  GGAGTACCTCATGGGAGGAGCCTACCGCTGCAACTACACTCGGAAAAACTTTCGGACCCTTTACAACAAC
  TTGTTTGGACCAAAGAGGCCTAAAGCTCTTAAACTTCTGGGAATGGAAGATGATGAGCCTCCAGCTAAAG
  GGAAGAAAAAGAAAAAGAAGAAAAAGGAGGAAGAGATCGACATTGATGTGGACGACCCTGCCGTGAGTCG
  GTTCCAGTATCCCTTCCACGAGCTGATGGTGTGGGCAGTGCTGATGAAACGCCAGAAAATGGCAGTGTTC
  CTCTGGCAGCGAGGGGAAGAGAGCATGGCCAAGGCCCTGGTGGCCTGCAAGCTCTACAAGGCCATGGCCC
  ACGAGTCCTCCGAGAGTGATCTGGTGGATGACATCTCCCAGGACTTGGATAACAATTCCAAAGACTTCGG
  CCAGCTTGCTTTGGAGTTATTAGACCAGTCCTATAAGCATGACGAGCAGATCGCTATGAAACTCCTGACC
  TACGAGCTGAAAAACTGGAGCAACTCGACCTGCCTCAAACTGGCCGTGGCAGCCAAACACCGGGACTTCA
  TTGCTCACACCTGCAGCCAGATGCTGCTGACCGATATGTGGATGGGAAGACTGCGGATGCGGAAGAACCC
  CGGCCTGAAGGTTATCATGGGGATTCTTCTACCCCCCACCATCTTGTTTTTGGAATTTCGCACATATGAT
  GATTTCTCGTATCAAACATCCAAGGAAAATGAGGATGGCAAAGAAAAAGAAGAGGAAAATACGGATGCAA
  ATGCAGATGCTGGCTCAAGAAAGGGGGATGAGGAGAACGAGCACAAAAAACAGAGAAGTATTCCCATCGG
  AACAAAGATCTGTGAATTCTATAACGCGCCCATTGTCAAGTTCTGGTTTTACACAATATCATACTTGGGC
  TACCTGCTGCTGTTTAACTACGTCATCCTGGTGCGGATGGATGGCTGGCCGTCCCTCCAGGAGTGGATCG
  TCATCTCCTACATCGTGAGCCTGGCGTTAGAGAAGATACGAGAGATCCTCATGTCAGAACCAGGCAAACT
  CAGCCAGAAAATCAAAGTTTGGCTTCAGGAGTACTGGAACATCACAGATCTCGTGGCCATTTCCACATTC
  ATGATTGGAGCAATTCTTCGCCTACAGAACCAGCCCTACATGGGCTATGGCCGGGTGATCTACTGTGTGG
  ATATCATCTTCTGGTACATCCGTGTCCTGGACATCTTTGGTGTCAACAAGTATCTGGGGCCATACGTGAT
  GATGATTGGAAAGATGATGATCGACATGCTGTACTTTGTGGTCATCATGCTGGTCGTGCTCATGAGTTTC
  GGAGTAGCCCGTCAAGCCATTCTGCATCCAGAGGAGAAGCCCTCTTGGAAACTGGCCCGAAACATCTTCT
  ACATGCCCTACTGGATGATCTATGGAGAGGTGTTTGCAGACCAGATAGACCTCTACGCCATGGAAATTAA
  TCCTCCTTGTGGTGAGAACCTATATGATGAGGAGGGCAAGCGGCTTCCTCCCTGTATCCCCGGCGCCTGG
  CTCACTCCAGCACTCATGGCGTGCTATCTACTGGTCGCCAACATCCTGCTGGTGAACCTGCTGATTGCTG
  TGTTCAACAATACCTTCTTTGAAGTAAAATCAATATCCAACCAGGTGTGGAAGTTCCAGCGATATCAGCT
  GATTATGACATTTCATGACAGGCCAGTCCTGCCCCCACCGATGATCATTTTAAGCCACATCTACATCATC
  ATTATGCGTCTCAGCGGCCGCTGCAGGAAAAAGAGAGAAGGGGACCAAGAGGAACGGGATCGTGGATTGA
  AGCTCTTCCTTAGCGACGAGGAGCTAAAGAGGCTGCATGAGTTCGAGGAGCAGTGCGTGCAGGAGCACTT
  CCGGGAGAAGGAGGATGAGCAGCAGTCGTCCAGCGACGAGCGCATCCGGGTCACTTCTGAAAGAGTTGAA
  AATATGTCAATGAGGTTGGAAGAAATCAATGAAAGAGAAACTTTTATGAAAACTTCCCTGCAGACTGTTG
  ACCTTCGACTTGCTCAGCTAGAAGAATTATCTAACAGAATGGTGAATGCTCTTGAAAATCTTGCGGGAAT
  CGACAGGTCTGACCTGATCCAGGCACGGTCCCGGGCTTCTTCTGAATGTGAGGCAACGTATCTTCTCCGG
  CAAAGCAGCATCAATAGCGCTGATGGCTACAGCTTGTATCGATATCATTTTAACGGAGAAGAGTTATTAT
  TTGAGGATACATCTCTCTCCACGTCACCAGGGACAGGAGTCAGGAAAAAAACCTGTTCCTTCCGTATAAA
  GGAAGAGAAGGACGTGAAAACGCACCTAGTCCCAGAATGTCAGAACAGTCTTCACCTTTCACTGGGCACA
  AGCACATCAGCAACCCCAGATGGCAGTCACCTTGCAGTAGATGACTTAAAGAACGCTGAAGAGTCAAAAT
  TAGGTCCAGATATTGGGATTTCAAAGGAAGATGATGAAAGACAGACAGACTCTAAAAAAGAAGAAACTAT
  TTCCCCAAGTTTAAATAAAACAGATGTGATACATGGACAGGACAAATCAGATGTTCAAAACACTCAGCTA
  ACAGTGGAAACGACAAATATAGAAGGCACTATTTCCTATCCCCTGGAAGAAACCAAAATTACACGCTATT
  TCCCCGATGAAACGATCAATGCTTGTAAAACAATGAAGTCCAGAAGCTTCGTCTATTCCCGGGGAAGAAA
  GCTGGTCGGTGGGGTTAACCAGGATGTAGAGTACAGTTCAATCACGGACCAGCAATTGACGACGGAATGG
  CAATGCCAAGTTCAAAAGATCACGCGCTCTCATAGCACAGATATTCCTTACATTGTGTCGGAAGCTGCAG
  TGCAAGCTGAGCATAAAGAGCAGTTTGCAGATATGCAAGATGAACACCATGTCGCTGAAGCAATTCCTCG
  AATCCCTCGCTTGTCCCTAACCATTACTGACAGAAATGGGATGGAAAACTTACTGTCTGTGAAGCCAGAT
  CAAACTTTGGGATTCCCATCTCTCAGGTCAAAAAGTTTACATGGACATCCTAGGAATGTGAAATCCATTC
  AGGGAAAGTTAGACAGATCTGGACATGCCAGTAGTGTAAGCAGCTTAGTAATTGTGTCTGGAATGACAGC
  AGAAGAAAAAAAGGTTAAGAAAGAGAAAGCTTCCACAGAAACTGAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213443 representing NM_002420
  Red=Cloning site Green=Tags(s)
  
  MKDSNRCCCGQFTNQHIPPLPSATPSKNEEESKQVETQPEKWSVAKHTQSYPTDSYGVLEFQGGGYSNKA
  MYIRVSYDTKPDSLLHLMVKDWQLELPKLLISVHGGLQNFEMQPKLKQVFGKGLIKAAMTTGAWIFTGGV
  STGVISHVGDALKDHSSKSRGRVCAIGIAPWGIVENKEDLVGKDVTRVYQTMSNPLSKLSVLNNSHTHFI
  LADNGTLGKYGAEVKLRRLLEKHISLQKINTRLGQGVPLVGLVVEGGPNVVSIVLEYLQEEPPIPVVICD
  GSGRASDILSFAHKYCEEGGIINESLREQLLVTIQKTFNYNKAQSHQLFAIIMECMKKKELVTVFRMGSE
  GQQDIEMAILTALLKGTNVSAPDQLSLALAWNRVDIARSQIFVFGPHWPPLGSLAPPTDSKATEKEKKPP
  MATTKGGRGKGKGKKKGKVKEEVEEETDPRKIELLNWVNALEQAMLDALVLDRVDFVKLLIENGVNMQHF
  LTIPRLEELYNTRLGPPNTLHLLVRDVKKSNLPPDYHISLIDIGLVLEYLMGGAYRCNYTRKNFRTLYNN
  LFGPKRPKALKLLGMEDDEPPAKGKKKKKKKKEEEIDIDVDDPAVSRFQYPFHELMVWAVLMKRQKMAVF
  LWQRGEESMAKALVACKLYKAMAHESSESDLVDDISQDLDNNSKDFGQLALELLDQSYKHDEQIAMKLLT
  YELKNWSNSTCLKLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNPGLKVIMGILLPPTILFLEFRTYD
  DFSYQTSKENEDGKEKEEENTDANADAGSRKGDEENEHKKQRSIPIGTKICEFYNAPIVKFWFYTISYLG
  YLLLFNYVILVRMDGWPSLQEWIVISYIVSLALEKIREILMSEPGKLSQKIKVWLQEYWNITDLVAISTF
  MIGAILRLQNQPYMGYGRVIYCVDIIFWYIRVLDIFGVNKYLGPYVMMIGKMMIDMLYFVVIMLVVLMSF
  GVARQAILHPEEKPSWKLARNIFYMPYWMIYGEVFADQIDLYAMEINPPCGENLYDEEGKRLPPCIPGAW
  LTPALMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHDRPVLPPPMIILSHIYII
  IMRLSGRCRKKREGDQEERDRGLKLFLSDEELKRLHEFEEQCVQEHFREKEDEQQSSSDERIRVTSERVE
  NMSMRLEEINERETFMKTSLQTVDLRLAQLEELSNRMVNALENLAGIDRSDLIQARSRASSECEATYLLR
  QSSINSADGYSLYRYHFNGEELLFEDTSLSTSPGTGVRKKTCSFRIKEEKDVKTHLVPECQNSLHLSLGT
  STSATPDGSHLAVDDLKNAEESKLGPDIGISKEDDERQTDSKKEETISPSLNKTDVIHGQDKSDVQNTQL
  TVETTNIEGTISYPLEETKITRYFPDETINACKTMKSRSFVYSRGRKLVGGVNQDVEYSSITDQQLTTEW
  QCQVQKITRSHSTDIPYIVSEAAVQAEHKEQFADMQDEHHVAEAIPRIPRLSLTITDRNGMENLLSVKPD
  QTLGFPSLRSKSLHGHPRNVKSIQGKLDRSGHASSVSSLVIVSGMTAEEKKVKKEKASTETEC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002420
• ORF Size: 4809 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002420.4, NM_002420.5, NP_002411.3
• RefSeq Size: 5428 bp
• RefSeq ORF: 4812 bp
• Locus ID: 4308
• Cytogenetics: 15q13.3
• Domains: ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• MW: 182 kDa
• Gene Summary: 'This gene encodes a member of the transient receptor potential melastatin subfamily of transient receptor potential ion channels. The encoded protein is a calcium permeable cation channel that is expressed in melanocytes and may play a role in melanin synthesis. Specific mutations in this gene are the cause autosomal recessive complete congenital stationary night blindness-1C. The expression of this protein is inversely correlated with melanoma aggressiveness and as such it is used as a prognostic marker for melanoma metastasis. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]'