CPAMD8 (NM_015692) Human Tagged ORF Clone

CAT#: RC213448

CPAMD8 (Myc-DDK-tagged)-Human C3 and PZP-like, alpha-2-macroglobulin domain containing 8 (CPAMD8)

Product Images

Western blot validation of overexpression lysate (Cat# LY414386) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213448 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CPAMD8
• Synonyms: ASGD8; K-CAP; VIP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213448 representing NM_015692
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC
  GCCC
  
  ATGGCGCCGGGCGCACTGCGCGGGGGCTGCGAACAAAGGGCCCCCGGCGGCGGCGCGAGGACGGCCGCGC
  TCGGACCCTGGCCCTGGCCCAGCCCTGGCCCGGCCCCCTCCCCAGGCGCGGCGCCCCCCAGGAGCCGAAA
  AATGAGCGGCGCCCTGCTCTGGCCGTTGCTCCCGCTCCTGCTCCTGCTGCTGTCGGCGCGGGACGGCGTG
  CGCGCCGCGCAGCCTCAGGCCCCGGGTTACTTGATTGCAGCTCCCTCTGTTTTTCGCGCGGGCGTGGAGG
  AAGTCATCAGCGTGACCATCTTTAACTCTCCAAGGGAAGTCACGGTCCAGGCTCAGCTGGTGGCCCAGGG
  TGAGCCGGTGGTGCAGAGCCAGGGAGCCATCCTGGATAAAGGGACAATCAAACTCAAGGTGCCCACGGGC
  CTCCGGGGCCAAGCGCTTCTGAAAGTGTGGGGCCGCGGCTGGCAGGCGGAGGAGGGGCCCCTCTTTCACA
  ACCAGACCTCGGTGACCGTGGACGGCCGGGGCGCTTCTGTATTCATCCAGACGGACAAGCCTGTGTACAG
  ACCCCAGCACCGAGTGCTCATAAGCATCTTCACCGTCTCTCCAAATCTGAGGCCTGTCAACGAGAAGCTG
  GAAGCCTACATCCTGGACCCCCGAGGCTCTCGGATGATAGAGTGGAGACACTTAAAGCCGTTCTGCTGCG
  GCATCACCAACATGAGCTTCCCCTTGTCCGACCAGCCTGTGTTGGGAGAATGGTTCATTTTTGTTGAAAT
  GCAAGGCCACGCGTACAACAAGTCTTTTGAAGTTCAGAAGTATGTGTTGCCCAAGTTTGAGCTTCTGATT
  GACCCGCCCCGGTATATCCAAGACCTGGACGCCTGTGAGACAGGCACTGTGCGGGCCAGGTATACCTTTG
  GGAAACCTGTGGCTGGTGCCTTAATGATCAACATGACTGTTAATGGTGTAGGGTACTACAGCCACGAGGT
  GGGACGCCCTGTCCTCAGAACAACCAAGATCCTCGGCTCCCGGGACTTCGACATCTGCGTGAGGGACATG
  ATCCCAGCGGACGTCCCTGAGCACTTCCGGGGCAGGGTCAGCATCTGGGCCATGGTGACCAGTGTGGACG
  GGAGCCAGCAGGTCGCGTTCGATGACTCCACCCCCGTGCAGAGGCAGCTGGTGGACATCCGGTACTCCAA
  GGACACGAGGAAGCAGTTCAAGCCGGGCCTGGCCTACGTGGGGAAGGTGGAGCTATCCTACCCCGATGGC
  AGCCCAGCTGAGGGGGTGACGGTCCAGATTAAGGCAGAGCTGACACCAAAGGATAACATCTACACCAGTG
  AAGTTGTGTCCCAGCGTGGACTAGTGGGGTTTGAAATCCCCTCCATCCCCACGTCAGCCCAGCACGTGTG
  GCTGGAGACCAAGGTGATGGCACTGAACGGGAAGCCTGTGGGGGCTCAGTACCTGCCCAGCTACCTCTCC
  CTCGGCAGCTGGTACTCCCCCAGCCAGTGCTACCTGCAGCTGCAGCCACCCTCCCACCCACTGCAGGTTG
  GGGAAGAAGCCTATTTTTCTGTGAAGTCCACATGTCCCTGCAACTTTACCCTGTACTACGAGGTGGCTGC
  ACGGGGCAATATTGTGCTATCGGGCCAGCAGCCTGCCCACACCACCCAGCAGCGAAGCAAGCGGGCGGCC
  CCTGCCCTGGAGAAACCGATTCGTTTAACACACCTTTCTGAGACAGAGCCCCCACCAGCCCCAGAAGCTG
  AGGTCGACGTGTGTGTGACCTCTCTTCATCTGGCCGTGACCCCCAGCATGGTCCCCCTTGGTCGCCTGCT
  GGTCTTCTACGTCAGGGAGAATGGAGAAGGGGTCGCCGACAGCCTTCAGTTTGCAGTCGAGACCTTCTTC
  GAAAACCAGGTTTCAGTGACGTATTCAGCAAATGAGACCCAACCTGGGGAGGTTGTCGACCTGCGGATCA
  GGGCTGCAAGGGGCAGCTGTGTGTGCGTCGCCGCAGTTGATAAGAGTGTCTACCTGCTCAGGTCTGGGTT
  CCGGCTGACTCCTGCCCAGGTTTTCCAGGAACTGGAAGATTATGATGTTTCTGATTCCTTTGGCGTGTCC
  AGGGAGGATGGTCCTTTTTGGTGGGCTGGGCTGACGGCACAACGACGCCGGCGCTCCTCTGTCTTCCCGT
  GGCCTTGGGGCATCACCAAGGACTCTGGGTTTGCCTTCACCGAAACGGGACTGGTGGTGATGACCGACCG
  AGTGAGCCTGAACCACCGGCAGGACGGTGGCCTCTACACCGATGAGGCTGTCCCCGCTTTCCAGCCCCAC
  ACAGGGAGCCTGGTGGCAGTGGCTCCTTCCAGGCACCCCCCCAGAACAGAGAAGAGAAAAAGGACTTTCT
  TCCCCGAAACATGGATTTGGCATTGTCTCAACATCAGTGACCCATCTGGTGAGGGGACACTCAGTGTGAA
  GGTCCCGGACTCCATCACCAGCTGGGTGGGTGAGGCCGTGGCCCTGTCCACCTCTCAGGGCTTAGGCATC
  GCCGAGCCCTCCCTGCTGAAGACCTTCAAGCCCTTCTTCGTGGACTTCATGCTCCCCGCTCTCATCATCC
  GTGGGGAGCAGGTCAAGATCCCGCTCAGTGTCTACAACTACATGGGCACCTGCGCTGAGGTGTACATGAA
  GCTCTCGGTTCCCAAGGGCATCCAGTTTGTTGGGCATCCTGGCAAACGCCATGTGACCAAGAAGATGTGT
  GTGGCCCCCGGGGAGGCTGAGCCCATCTGGGTCGTTCTGTCCTTCAGCGACCTGGGACTCAACAACATCA
  CGGCCAAAGCCCTTGCTTACGGAGACACAAATTGCTGCCGGGATGGGAGGTCCAGCAAACACCCTGAGGA
  GAATCACGCCGACAGGAGGGTCCCCATCGGGGTGGATCACGTCAGGCGCAGTGTGATGGTTGAGGCGGAA
  GGAGTCCCCCGGGCGTACACCTACAGCGCATTCTTCTGTCCCAGTGAGAGAGTCCACATCTCCACCCCCA
  ACAAGTATGAGTTCCAGTATGTGCAGCGGCCACTGCGCCTCACCCGCTTTGATGTGGCTGTGCGAGCTCA
  CAATGATGCCCGTGTGGCCTTGTCTTCTGGGCCCCAGGACACAGCAGGCATGATCGAGATCGTCCTGGGG
  GGGCATCAGAACACCAGGTCATGGATCTCCACCAGCAAGATGGGAGAGCCCGTGGCCAGTGCACACACGG
  CCAAGATCCTCTCCTGGGATGAATTCAGAACATTCTGGATCAGCTGGCGTGGTGGCCTTATCCAGGTTGG
  CCATGGTCCAGAGCCATCCAATGAGTCTGTCATTGTGGCCTGGACCCTCCCGAGGCCACCAGAGGTCCAG
  TTCATTGGCTTTTCCACCGGCTGGGGCTCCATGGGTGAATTCCGAATCTGGAGGAAGATGGAGGTGGACG
  AGAGCTACAGCGAGGCCTTCACCCTGGGGGTCCCACACGGCGCCATCCCTGGGTCTGAGCGAGCCACCGC
  CTCCATCATCGGGGACGTCATGGGGCCAACCCTGAACCACCTCAACAACCTCCTGCGGCTGCCGTTTGGC
  TGTGGAGAGCAGAACATGATCCACTTTGCACCCAACGTCTTTGTCTTGAAGTATCTTCAGAAAACCCAGC
  AGCTCAGCCCTGAGGTGGAGAGAGAGACCACCGACTACCTAGTACAAGGCTACCAGCGCCAGCTGACCTA
  CAAGCGCCAGGATGGCTCCTACAGCGCGTTTGGGGAGCGGGACGCATCGGGGAGCATGTGGCTCACAGCC
  TTTGTCCTGAAGTCCTTCGCACAGGCTCGCAGCTTTATCTTCGTGGACCCCCGGGAGCTGGCTGCCGCCA
  AGAGCTGGATCATCCAGCAGCAGCAGGCCGATGGCTCCTTCCTGGCCGTGGGCAGGGTCCTGAACAAGGA
  CATCCAGGGTGGGATCCACGGCACTGTCCCGCTGACAGCCTACGTGGTGGTTGCTCTCCTGGAAACAGGC
  ACAGCCTCAGAGGAGGAGAGAGGCTCCACTGACAAAGCGAGGCACTTCCTGGAGTCTGCTGCGCCCCTGG
  CCATGGACCCTTATAGCTGTGCCCTGACTACCTACGCGCTGACCCTGCTCCGCAGCCCGGCAGCCCCTGA
  GGCACTGCGCAAGCTCCGTAGCCTGGCCATCATGCGAGATGGGGTCACCCACTGGAGCCTGTCAAATTCC
  TGGGACGTGGACAAGGGCACATTCTTGAGCTTCAGTGACAGGGTCTCTCAGTCAGTGGTCTCGGCCGAGG
  TGGAAATGACAGCCTACGCCCTTCTGACCTACACTCTGCTGGGTGACGTGGCTGCCGCCCTGCCTGTGGT
  GAAGTGGCTGTCCCAGCAGCGAAATGCACTTGGGGGCTTCTCCTCCACTCAGGACACCTGCGTGGCTCTG
  CAGGCCTTGGCTGAATATGCCATCTTGTCCTATGCTGGAGGCATCAACCTCACTGTCTCCCTGGCCTCCA
  CCAACCTGGACTACCAGGAAACCTTCGAGCTGCACAGGACCAACCAGAAGGTTCTGCAGACAGCAGCGAT
  CCCCAGCCTCCCCACGGGGCTGTTTGTGAGTGCCAAGGGGGACGGCTGCTGCCTGATGCAGATTGATGTC
  ACCTACAATGTGCCTGACCCGGTGGCCAAGCCAGCTTTCCAGCTGCTCGTAAGCCTCCAGGAGCCTGAGG
  CCCAGGGACGCCCGCCCCCCATGCCTGCCTCCGCAGCTGAGGGTTCCCGAGGAGACTGGCCCCCAGCTGA
  CGATGATGACCCAGCGGCCGATCAGCATCACCAGGAATACAAGGTGATGCTGGAGGTGTGCACCAGGTGG
  CTGCATGCAGGGTCTTCCAATATGGCTGTCCTGGAGGTGCCCCTGCTGTCAGGCTTCCGGGCAGACATCG
  AGAGCCTGGAGCAGCTGCTCCTTGACAAGCACATGGGGATGAAGAGGTATGAAGTGGCTGGACGCCGAGT
  GCTCTTCTACTTTGATGAGATCCCCAGCCGGTGCCTGACGTGCGTGCGGTTCCGTGCTCTCCGGGAGTGC
  GTGGTGGGCAGGACGTCGGCGCTGCCAGTCTCCGTGTACGACTACTACGAACCCGCCTTCGAGGCCACTC
  GCTTCTACAACGTCAGCACCCACAGCCCACTCGCCCGGGAACTGTGCGCCGGACCCGCGTGCAACGAAGT
  GGAGCGCGCCCCTGCCCGGGGCCCGGGCTGGTTCCCCGGCGAGTCGGGCCCTGCCGTGGCCCCTGAGGAG
  GGGGCGGCGATCGCGCGATGCGGCTGCGACCACGACTGCGGCGCCCAGGGGAACCCGGTGTGCGGCTCCG
  ACGGGGTGGTCTACGCCAGCGCCTGCCGCCTGCGGGAGGCCGCCTGCCGCCAGGCCGCGCCCCTGGAGCC
  CGCGCCTCCCAGCTGCTGCGCCCTCGAGCAGCGGCTGCCGGCCTCGTCGTCCTCCACCTACGGGGATGAC
  CTGGCTTCTGTGGCCCCGGGGCCTTTACAGCAGGACGTGAAGCTGAATGGAGCCGGCCTTGAGGTGGAGG
  ACTCAGACCCTGAGCCTGAAGGGGAGGCGGAGGACAGGGTCACAGCCGGGCCTCGGCCTCCTGTGAGCAG
  CGGGAACCTGGAAAGCAGCACCCAGAGCGCCAGCCCGTTCCACAGATGGGGCCAGACTCCGGCCCCTCAG
  AGACATAGTGGCCGGGTGGTGGGGGCCCACAGGCCAGGGCTTCTGAGCCCTGTCTTCGTCTACAGCCCAG
  CCTTTCAGAGTGGTGGGGAGGAGGGTTTATGGATGTCAAACACCTGCACCTTGAGA
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213448 representing NM_015692
  Red=Cloning site Green=Tags(s)
  
  MAPGALRGGCEQRAPGGGARTAALGPWPWPSPGPAPSPGAAPPRSRKMSGALLWPLLPLLLLLLSARDGV
  RAAQPQAPGYLIAAPSVFRAGVEEVISVTIFNSPREVTVQAQLVAQGEPVVQSQGAILDKGTIKLKVPTG
  LRGQALLKVWGRGWQAEEGPLFHNQTSVTVDGRGASVFIQTDKPVYRPQHRVLISIFTVSPNLRPVNEKL
  EAYILDPRGSRMIEWRHLKPFCCGITNMSFPLSDQPVLGEWFIFVEMQGHAYNKSFEVQKYVLPKFELLI
  DPPRYIQDLDACETGTVRARYTFGKPVAGALMINMTVNGVGYYSHEVGRPVLRTTKILGSRDFDICVRDM
  IPADVPEHFRGRVSIWAMVTSVDGSQQVAFDDSTPVQRQLVDIRYSKDTRKQFKPGLAYVGKVELSYPDG
  SPAEGVTVQIKAELTPKDNIYTSEVVSQRGLVGFEIPSIPTSAQHVWLETKVMALNGKPVGAQYLPSYLS
  LGSWYSPSQCYLQLQPPSHPLQVGEEAYFSVKSTCPCNFTLYYEVAARGNIVLSGQQPAHTTQQRSKRAA
  PALEKPIRLTHLSETEPPPAPEAEVDVCVTSLHLAVTPSMVPLGRLLVFYVRENGEGVADSLQFAVETFF
  ENQVSVTYSANETQPGEVVDLRIRAARGSCVCVAAVDKSVYLLRSGFRLTPAQVFQELEDYDVSDSFGVS
  REDGPFWWAGLTAQRRRRSSVFPWPWGITKDSGFAFTETGLVVMTDRVSLNHRQDGGLYTDEAVPAFQPH
  TGSLVAVAPSRHPPRTEKRKRTFFPETWIWHCLNISDPSGEGTLSVKVPDSITSWVGEAVALSTSQGLGI
  AEPSLLKTFKPFFVDFMLPALIIRGEQVKIPLSVYNYMGTCAEVYMKLSVPKGIQFVGHPGKRHVTKKMC
  VAPGEAEPIWVVLSFSDLGLNNITAKALAYGDTNCCRDGRSSKHPEENHADRRVPIGVDHVRRSVMVEAE
  GVPRAYTYSAFFCPSERVHISTPNKYEFQYVQRPLRLTRFDVAVRAHNDARVALSSGPQDTAGMIEIVLG
  GHQNTRSWISTSKMGEPVASAHTAKILSWDEFRTFWISWRGGLIQVGHGPEPSNESVIVAWTLPRPPEVQ
  FIGFSTGWGSMGEFRIWRKMEVDESYSEAFTLGVPHGAIPGSERATASIIGDVMGPTLNHLNNLLRLPFG
  CGEQNMIHFAPNVFVLKYLQKTQQLSPEVERETTDYLVQGYQRQLTYKRQDGSYSAFGERDASGSMWLTA
  FVLKSFAQARSFIFVDPRELAAAKSWIIQQQQADGSFLAVGRVLNKDIQGGIHGTVPLTAYVVVALLETG
  TASEEERGSTDKARHFLESAAPLAMDPYSCALTTYALTLLRSPAAPEALRKLRSLAIMRDGVTHWSLSNS
  WDVDKGTFLSFSDRVSQSVVSAEVEMTAYALLTYTLLGDVAAALPVVKWLSQQRNALGGFSSTQDTCVAL
  QALAEYAILSYAGGINLTVSLASTNLDYQETFELHRTNQKVLQTAAIPSLPTGLFVSAKGDGCCLMQIDV
  TYNVPDPVAKPAFQLLVSLQEPEAQGRPPPMPASAAEGSRGDWPPADDDDPAADQHHQEYKVMLEVCTRW
  LHAGSSNMAVLEVPLLSGFRADIESLEQLLLDKHMGMKRYEVAGRRVLFYFDEIPSRCLTCVRFRALREC
  VVGRTSALPVSVYDYYEPAFEATRFYNVSTHSPLARELCAGPACNEVERAPARGPGWFPGESGPAVAPEE
  GAAIARCGCDHDCGAQGNPVCGSDGVVYASACRLREAACRQAAPLEPAPPSCCALEQRLPASSSSTYGDD
  LASVAPGPLQQDVKLNGAGLEVEDSDPEPEGEAEDRVTAGPRPPVSSGNLESSTQSASPFHRWGQTPAPQ
  RHSGRVVGAHRPGLLSPVFVYSPAFQSGGEEGLWMSNTCTLR
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: AscI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015692
• ORF Size: 5796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015692.2, NP_056507.2
• RefSeq Size: 5996
• RefSeq ORF: 5658
• Locus ID: 27151
• MW: 211.1 kDa
• Gene Summary: This gene encodes a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. These proteins are important in innate and acquired immunity. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. [provided by RefSeq, May 2017]