BCOR (NM_017745) Human Tagged ORF Clone

CAT#: RC213468

BCOR (Myc-DDK-tagged)-Human BCL6 corepressor (BCOR), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY426616) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC226424 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCOR
• Synonyms: ANOP2; MAA2; MCOPS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213468 representing NM_017745
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCTCAGCAACCCCCCTGTATGGGAACGTTCACAGCTGGATGAACAGCGAGAGGGTCCGCATGTGTG
  GGGCGAGCGAAGACAGGAAAATCCTTGTAAATGATGGTGACGCTTCAAAAGCCAGACTGGAACTGAGGGA
  AGAGAATCCCTTGAACCACAACGTGGTGGATGCGAGCACGGCCCATAGGATCGATGGCCTGGCAGCACTG
  AGCATGGACCGCACTGGCCTGATCCGGGAAGGGCTGCGGGTCCCGGGAAACATCGTCTATTCTAGCTTGT
  GTGGACTGGGCTCAGAGAAAGGTCGGGAGGCTGCCACAAGCACTCTAGGTGGCCTTGGGTTTTCTTCGGA
  AAGAAATCCAGAGATGCAGTTCAAACCGAATACACCCGAGACAGTGGAGGCTTCTGCCGTCTCTGGAAAA
  CCCCCAAATGGCTTCAGTGCTATATACAAAACACCGCCTGGAATACAAAAAAGTGCTGTAGCCACAGCAG
  AAGCGCTGGGCTTGGACAGGCCTGCCAGCGACAAACAGAGCCCTCTCAACATCAATGGTGCTAGTTATCT
  GCGGCTGCCCTGGGTCAATCCTTACATGGAGGGTGCCACGCCAGCCATCTACCCTTTCCTCGACTCGCCA
  AATAAGTATTCACTGAACATGTACAAGGCCTTGCTACCTCAGCAGTCCTACAGCTTGGCCCAGCCGCTGT
  ATTCTCCAGTCTGCACCAATGGGGAGCGCTTTCTCTACCTGCCGCCACCTCACTACGTCGGTCCCCACAT
  CCCATCGTCCTTGGCATCACCCATGAGGCTCTCGACACCTTCGGCCTCCCCAGCCATCCCGCCTCTCGTC
  CATTGCGCAGACAAAAGCCTCCCGTGGAAGATGGGCGTCAGCCCTGGGAATCCTGTTGATTCCCACGCCT
  ATCCTCACATCCAGAACAGTAAGCAGCCCAGGGTTCCCTCTGCCAAGGCGGTCACCAGTGGCCTGCCGGG
  GGACACAGCTCTCCTGTTGCCCCCCTCGCCTCGGCCGTCACCCCGAGTCCACCTTCCCACCCAGCCTGCT
  GCAGACACCTACTCGGAGTTCCACAAGCACTATGCCAGGATCTCCACCTCTCCTTCAGTTGCCCTGTCAA
  AGCCATACATGACAGTTAGCAGCGAGTTCCCCGCGGCCAGGCTCTCCAATGGCAAGTATCCCAAGGCTCC
  GGAAGGGGGCGAAGGTGCCCAGCCAGTGCCCGGGCATGCCCGGAAGACAGCGGTTCAAGACAGAAAAGAC
  GGCAGCTCACCTCCTCTGTTGGAGAAGCAGACCGTTACCAAAGACGTCACAGATAAGCCACTAGACTTGT
  CTTCTAAAGTGGTGGATGTAGATGCTTCCAAAGCTGACCACATGAAAAAGATGGCTCCCACGGTCCTGGT
  TCACAGCAGGGCTGGAAGTGGCTTAGTGCTCTCCGGAAGTGAGATTCCGAAAGAAACACTATCTCCTCCA
  GGAAATGGTTGTGCTATCTATAGATCTGAAATCATCAGCACTGCTCCCTCATCCTGGGTGGTGCCCGGGC
  CAAGTCCTAACGAAGAGAACAATGGCAAAAGCATGTCGCTGAAAAACAAGGCATTGGACTGGGCGATACC
  ACAGCAGCGGAGTTCATCATGCCCGCGCATGGGCGGCACCGATGCTGTCATCACTAACGTTTCAGGGTCA
  GTGTCGAGTGCAGGCCGCCCAGCCTCCGCATCACCCGCCCCCAATGCCAATGCAGATGGCACCAAAACCA
  GCAGGAGCTCTGTAGAAACCACACCATCCGTTATTCAGCACGTGGGCCAGCCCCCGGCCACTCCTGCCAA
  GCACAGTAGCAGCACCAGCAGCAAGGGCGCCAAAGCCAGCAACCCAGAACCGAGTTTCAAAGCAAACGAG
  AACGGCCTTCCACCAAGCTCTATATTTCTGTCTCCAAATGAGGCATTCAGGTCCCCACCAATTCCCTACC
  CCAGGAGTTACCTCCCTTACCCAGCCCCTGAGGGCATTGCTGTAAGTCCCCTCTCCTTACATGGCAAAGG
  ACCTGTCTACCCTCACCCAGTTTTGTTACCCAATGGCAGTCTGTTTCCTGGGCACCTTGCCCCAAAGCCT
  GGGCTGCCCTATGGGCTTCCCACCGGCCGTCCAGAGTTTGTGACCTACCAAGATGCCCTGGGGTTGGGCA
  TGGTGCATCCCATGTTGATACCACACACGCCCATAGAGATTACTAAAGAGGAGAAACCAGAGAGGAGATC
  CCGGTCCCATGAGAGAGCCCGTTACGAGGACCCAACCCTCCGGAATCGGTTTTCCGAGATTTTGGAAACT
  AGCAGCACCAAGTTACATCCAGATGTCCCCACCGACAAGAACCTAAAGCCGAACCCCAACTGGAATCAAG
  GGAAGACTGTTGTCAAAAGCGACAAGCTTGTCTACGTAGACCTTCTCCGAGAAGAACCAGATGCTAAAAC
  TGACACAAACGTGTCCAAACCCAGCTTTGCAGCAGAGAGTGTTGGCCAGAGCGCTGAGCCCCCCAAGCCC
  TCAGTTGAGCCGGCCCTGCAGCAGCACCGTGATTTCATCGCCCTGAGAGAGGAGTTGGGGCGCATCAGTG
  ACTTCCACGAAACTTATACTTTCAAACAGCCAGTCTTCACCGTAAGCAAGGACAGTGTTCTGGCAGGTAC
  CAACAAAGAGAACCTAGGGTTGCCAGTCTCGACTCCATTCCTGGAGCCACCTCTGGGGAGCGATGGCCCT
  GCTGTAACTTTTGGTAAAACCCAAGAGGATCCCAAACCATTTTGTGTGGGCAGTGCCCCACCAAGTGTGG
  ATGTGACCCCCACCTATACCAAAGATGGAGCTGATGAGGCTGAATCAAATGATGGCAAAGTTCTGAAACC
  GAAGCCATCTAAGCTGGCAAAGAGAATCGCCAACTCAGCGGGTTACGTGGGTGACCGATTCAAATGTGTC
  ACTACCGAACTGTATGCAGATTCCAGTCAGCTCAGCCGGGAGCAACGGGCATTGCAGATGGAAGGATTAC
  AAGAGGACAGTATTTTATGTCTACCCGCTGCTTACTGTGAGCGTGCAATGATGCGCTTCTCAGAGTTGGA
  GATGAAAGAAAGAGAAGGTGGCCACCCAGCAACCAAAGACTCCGAGATGTGCAAATTCAGCCCAGCCGAC
  TGGGAAAGGTTGAAAGGAAATCAGGACAAAAAGCCAAAGTCGGTCACCCTGGAGGAGGCCATTGCAGAAC
  AGAACGAAAGTGAGAGATGCGAGTATAGTGTTGGAAACAAGCACCGTGATCCCTTTGAAGCCCCAGAGGA
  CAAAGATCTTCCTGTGGAGAAGTACTTTGTGGAGAGGCAGCCTGTGAGCGAGCCTCCCGCAGACCAGGTG
  GCCTCGGACATGCCTCACAGCCCCACCCTCCGGGTGGACAGGAAACGCAAAGTCTCAGGTGACAGCAGCC
  ACACTGAGACCACTGCGGAGGAGGTGCCAGAGGACCCTCTGCTGAAAGCCAAACGCCGACGAGTCTCTAA
  AGGGCTCCATCCTAAAAAACAACGCCACTTGCTGCACCTTAGAGAACGATGGGAGCAGCAGGTGTCGGCA
  GCAGATGGCAAACCTGGCCGGCAAAGCAGGAAGGAAGTGACCCAGGCCACTCAGCCTGAGGCCATTCCTC
  AGGGGACTAACATCACTGAAGAGAAACCTGGCAGGAAAAGGGCAGAGGCCAAAGGCAACAGAAGCTGGTC
  GGAAGAGTCTCTTAAACCCAGTGACAATGAACAAGGCTTGCCTGTGTTCTCCGGCTCTCCGCCCATGAAG
  AGTCTTTCATCCACCAGTGCAGGCGGCAAAAAGCAGGCTCAGCCAAGCTGCGCACCAGCCTCCAGGCCGC
  CTGCCAAACAGCAGAAAATTAAAGAAAACCAGAAGACAGATGTGCTGTGTGCAGACGAAGAAGAGGATTG
  CCAGGCTGCCTCCCTGCTGCAGAAATACACCGACAACAGCGAGAAGCCATCCGGGAAGAGACTGTGCAAA
  ACCAAACACTTGATCCCTCAGGAGTCCAGGCGGGGATTGCCACTGACAGGGGAATACTACGTGGAGAATG
  CCGATGGCAAGGTGACTGTCCGGAGATTCAGAAAGCGGCCGGAGCCCAGTTCGGACTATGATCTGTCACC
  AGCCAAGCAGGAGCCAAAGCCCTTCGACCGCTTGCAGCAACTGCTACCAGCCTCCCAGTCCACACAGCTG
  CCATGCTCAAGTTCCCCTCAGGAGACCACCCAGTCTCGCCCTATGCCGCCGGAAGCACGGAGACTTATTG
  TCAATAAGAACGCTGGCGAGACCCTTCTGCAGCGGGCAGCCAGGCTTGGCTATGAGGAAGTGGTCCTGTA
  CTGCTTAGAGAACAAGATTTGTGATGTAAATCATCGGGACAACGCAGGTTACTGCGCCCTGCATGAAGCT
  TGTGCTAGGGGCTGGCTCAACATTGTGCGACACCTCCTTGAATATGGCGCTGATGTCAACTGTAGTGCCC
  AGGATGGAACCAGGCCTCTGCACGATGCTGTTGAGAACGATCACTTGGAAATTGTCCGACTACTTCTCTC
  TTATGGTGCTGACCCCACCTTGGCTACGTACTCAGGTAGAACCATCATGAAAATGACCCACAGTGAACTT
  ATGGAAAAGTTCTTAACAGATTATTTAAATGACCTCCAGGGTCGCAATGATGATGACGCCAGTGGCACTT
  GGGACTTCTATGGCAGCTCTGTTTGTGAACCAGATGATGAAAGTGGCTATGATGTTTTAGCCAACCCCCC
  AGGACCAGAAGACCAGGATGATGATGACGATGCCTATAGCGATGTGTTTGAATTTGAATTTTCAGAGACC
  CCCCTCTTACCGTGTTATAACATCCAAGTATCTGTGGCTCAGGGGCCACGAAACTGGCTACTGCTTTCGG
  ATGTCCTTAAGAAATTGAAAATGTCCTCCCGCATATTTCGCTGCAATTTTCCAAACGTGGAAATTGTCAC
  CATTGCAGAGGCAGAATTTTATCGGCAGGTTTCTGCAAGTCTCTTGTTCTCTTGCTCCAAAGACCTGGAA
  GCCTTCAACCCTGAAAGTAAGGAGCTGTTAGATCTGGTGGAATTCACGAACGAAATTCAGACTCTGCTGG
  GCTCCTCTGTAGAGTGGCTCCACCCCAGTGATCTGGCCTCAGACAACTACTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213468 representing NM_017745
  Red=Cloning site Green=Tags(s)
  
  MLSATPLYGNVHSWMNSERVRMCGASEDRKILVNDGDASKARLELREENPLNHNVVDASTAHRIDGLAAL
  SMDRTGLIREGLRVPGNIVYSSLCGLGSEKGREAATSTLGGLGFSSERNPEMQFKPNTPETVEASAVSGK
  PPNGFSAIYKTPPGIQKSAVATAEALGLDRPASDKQSPLNINGASYLRLPWVNPYMEGATPAIYPFLDSP
  NKYSLNMYKALLPQQSYSLAQPLYSPVCTNGERFLYLPPPHYVGPHIPSSLASPMRLSTPSASPAIPPLV
  HCADKSLPWKMGVSPGNPVDSHAYPHIQNSKQPRVPSAKAVTSGLPGDTALLLPPSPRPSPRVHLPTQPA
  ADTYSEFHKHYARISTSPSVALSKPYMTVSSEFPAARLSNGKYPKAPEGGEGAQPVPGHARKTAVQDRKD
  GSSPPLLEKQTVTKDVTDKPLDLSSKVVDVDASKADHMKKMAPTVLVHSRAGSGLVLSGSEIPKETLSPP
  GNGCAIYRSEIISTAPSSWVVPGPSPNEENNGKSMSLKNKALDWAIPQQRSSSCPRMGGTDAVITNVSGS
  VSSAGRPASASPAPNANADGTKTSRSSVETTPSVIQHVGQPPATPAKHSSSTSSKGAKASNPEPSFKANE
  NGLPPSSIFLSPNEAFRSPPIPYPRSYLPYPAPEGIAVSPLSLHGKGPVYPHPVLLPNGSLFPGHLAPKP
  GLPYGLPTGRPEFVTYQDALGLGMVHPMLIPHTPIEITKEEKPERRSRSHERARYEDPTLRNRFSEILET
  SSTKLHPDVPTDKNLKPNPNWNQGKTVVKSDKLVYVDLLREEPDAKTDTNVSKPSFAAESVGQSAEPPKP
  SVEPALQQHRDFIALREELGRISDFHETYTFKQPVFTVSKDSVLAGTNKENLGLPVSTPFLEPPLGSDGP
  AVTFGKTQEDPKPFCVGSAPPSVDVTPTYTKDGADEAESNDGKVLKPKPSKLAKRIANSAGYVGDRFKCV
  TTELYADSSQLSREQRALQMEGLQEDSILCLPAAYCERAMMRFSELEMKEREGGHPATKDSEMCKFSPAD
  WERLKGNQDKKPKSVTLEEAIAEQNESERCEYSVGNKHRDPFEAPEDKDLPVEKYFVERQPVSEPPADQV
  ASDMPHSPTLRVDRKRKVSGDSSHTETTAEEVPEDPLLKAKRRRVSKGLHPKKQRHLLHLRERWEQQVSA
  ADGKPGRQSRKEVTQATQPEAIPQGTNITEEKPGRKRAEAKGNRSWSEESLKPSDNEQGLPVFSGSPPMK
  SLSSTSAGGKKQAQPSCAPASRPPAKQQKIKENQKTDVLCADEEEDCQAASLLQKYTDNSEKPSGKRLCK
  TKHLIPQESRRGLPLTGEYYVENADGKVTVRRFRKRPEPSSDYDLSPAKQEPKPFDRLQQLLPASQSTQL
  PCSSSPQETTQSRPMPPEARRLIVNKNAGETLLQRAARLGYEEVVLYCLENKICDVNHRDNAGYCALHEA
  CARGWLNIVRHLLEYGADVNCSAQDGTRPLHDAVENDHLEIVRLLLSYGADPTLATYSGRTIMKMTHSEL
  MEKFLTDYLNDLQGRNDDDASGTWDFYGSSVCEPDDESGYDVLANPPGPEDQDDDDDAYSDVFEFEFSET
  PLLPCYNIQVSVAQGPRNWLLLSDVLKKLKMSSRIFRCNFPNVEIVTIAEAEFYRQVSASLLFSCSKDLE
  AFNPESKELLDLVEFTNEIQTLLGSSVEWLHPSDLASDNYW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017745
• ORF Size: 5163 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017745.2, NM_017745.4, NM_017745.5, NP_060215.4
• RefSeq Size: 6182 bp
• RefSeq ORF: 5166 bp
• Locus ID: 54880
• Domains: ANK
• Protein Families: Transcription Factors
• MW: 188 kDa
• Gene Summary: The protein encoded by this gene was identified as an interacting corepressor of BCL6, a POZ/zinc finger transcription repressor that is required for germinal center formation and may influence apoptosis. This protein selectively interacts with the POZ domain of BCL6, but not with eight other POZ proteins. Specific class I and II histone deacetylases (HDACs) have been shown to interact with this protein, which suggests a possible link between the two classes of HDACs. Several transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome Y. [provided by RefSeq, Jun 2010]