ACOT7 (NM_181864) Human Tagged ORF Clone

CAT#: RC213596

ACOT7 (Myc-DDK-tagged)-Human acyl-CoA thioesterase 7 (ACOT7), transcript variant hBACHb

Product Images

Western blot validation of overexpression lysate (Cat# LY405559) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213596 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACOT7
• Synonyms: ACH1; ACT; BACH; CTE-II; hBACH; LACH; LACH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213596 representing NM_181864
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCTGCTTGCCAGGGCTCTCCGGCTCTGTGAGTTTGGGAGGCAGGCATCTTCCAGGAGGCTGGTGG
  CTGGCCAGGGATGTGTGGGGCCCCGGCGAGGGTGCTGCGCTCCCGTCCAGGTGGTTGGGCCCAGGGCTGA
  TCTCCCACCCTGTGGAGCCTGCATTACTGGAAGGATCATGCGGCCAGATGATGCCAACGTGGCCGGCAAT
  GTCCACGGGGGGACCATCCTGAAGATGATCGAGGAGGCAGGCGCCATCATCAGCACCCGGCATTGCAACA
  GCCAGAACGGGGAGCGCTGTGTGGCCGCCCTGGCTCGTGTCGAGCGCACCGACTTCCTGTCTCCCATGTG
  CATCGGTGAGGTGGCGCATGTCAGCGCGGAGATCACCTACACCTCCAAGCACTCTGTGGAGGTGCAGGTC
  AACGTGATGTCCGAAAACATCCTCACAGGTGCCAAAAAGCTGACCAATAAGGCCACCCTGTGGTATGTGC
  CCCTGTCGCTGAAGAATGTGGACAAGGTCCTCGAGGTGCCTCCTGTTGTGTATTCCCGGCAGGAGCAGGA
  GGAGGAGGGCCGGAAGCGGTATGAAGCCCAGAAGCTGGAGCGCATGGAGACCAAGTGGAGGAACGGGGAC
  ATCGTCCAGCCAGTCCTCAACCCAGAGCCGAACACTGTCAGCTACAGCCAGTCCAGCTTGATCCACCTGG
  TGGGGCCTTCAGACTGCACCCTGCACGGCTTTGTGCACGGAGGTGTGACCATGAAGCTCATGGATGAGGT
  CGCCGGGATCGTGGCTGCACGCCACTGCAAGACCAACATCGTCACAGCTTCCGTGGACGCCATTAATTTT
  CATGACAAGATCAGAAAAGGCTGCGTCATCACCATCTCGGGACGCATGACCTTCACGAGCAATAAGTCCA
  TGGAGATCGAGGTGTTGGTGGACGCCGACCCTGTTGTGGACAGCTCTCAGAAGCGCTACCGGGCCGCCAG
  TGCCTTCTTCACCTACGTGTCGCTGAGCCAGGAAGGCAGGTCGCTGCCTGTGCCCCAGCTGGTGCCCGAG
  ACCGAGGACGAGAAGAAGCGCTTTGAGGAAGGCAAAGGGCGGTACCTGCAGATGAAGGCGAAGCGACAGG
  GCCACGCGGAGCCTCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213596 representing NM_181864
  Red=Cloning site Green=Tags(s)
  
  MKLLARALRLCEFGRQASSRRLVAGQGCVGPRRGCCAPVQVVGPRADLPPCGACITGRIMRPDDANVAGN
  VHGGTILKMIEEAGAIISTRHCNSQNGERCVAALARVERTDFLSPMCIGEVAHVSAEITYTSKHSVEVQV
  NVMSENILTGAKKLTNKATLWYVPLSLKNVDKVLEVPPVVYSRQEQEEEGRKRYEAQKLERMETKWRNGD
  IVQPVLNPEPNTVSYSQSSLIHLVGPSDCTLHGFVHGGVTMKLMDEVAGIVAARHCKTNIVTASVDAINF
  HDKIRKGCVITISGRMTFTSNKSMEIEVLVDADPVVDSSQKRYRAASAFFTYVSLSQEGRSLPVPQLVPE
  TEDEKKRFEEGKGRYLQMKAKRQGHAEPQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181864
• ORF Size: 1140 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_181864.1, NM_181864.2, NP_863654.1
• RefSeq Size: 1634
• RefSeq ORF: 1143
• Locus ID: 11332
• Protein Pathways: Biosynthesis of unsaturated fatty acids
• MW: 41.6 kDa
• Gene Summary: This gene encodes a member of the acyl coenzyme family. The encoded protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this gene may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. [provided by RefSeq, Jul 2008]