E2F8 (NM_024680) Human Tagged ORF Clone
CAT#: RC213597
E2F8 (Myc-DDK-tagged)-Human E2F transcription factor 8 (E2F8)
"NM_024680" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | E2F8 |
Synonyms | E2F-8 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213597 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGAACGAAAAGGAAAATCTCTTTTGTGAGCCACATAAAAGGGGACTAATGAAAACACCTCTGAAAG AATCCACCACAGCAAATATCGTGTTGGCAGAGATCCAGCCTGACTTTGGCCCTTTAACCACACCTACCAA GCCCAAGGAAGGCTCTCAGGGAGAGCCGTGGACACCGACAGCCAACCTGAAAATGCTCATCAGTGCTGTG AGCCCTGAGATCCGCAACAGAGATCAGAAAAGGGGTTTGTTTGACAACAGAAGTGGATTACCTGAGGCCA AAGACTGTATACACGAACACTTATCTGGAGATGAATTTGAGAAATCCCAACCAAGTCGAAAAGAGAAAAG TTTAGGATTATTGTGTCATAAGTTCTTAGCACGATATCCTAATTATCCCAACCCTGCTGTGAATAATGAC ATCTGCCTTGACGAAGTGGCAGAGGAACTTAATGTTGAACGTCGACGCATTTACGATATCGTGAACGTCC TAGAGAGTTTACATATGGTGAGCCGCCTCGCCAAAAACAGGTACACTTGGCACGGGCGACACAATCTCAA CAAAACCCTTGGCACCTTGAAGAGCATCGGGGAGGAGAATAAGTACGCCGAGCAGATTATGATGATCAAA AAGAAAGAATATGAGCAAGAGTTTGACTTTATTAAGAGTTACAGTATAGAGGATCATATCATCAAATCAA ACACTGGCCCAAATGGACACCCAGACATGTGTTTTGTGGAACTCCCTGGAGTGGAATTTCGGGCAGCTTC TGTAAACAGCCGCAAAGACAAGTCTTTAAGGGTAATGAGCCAGAAATTTGTGATGCTGTTTTTGGTGTCA ACGCCTCAGATAGTAAGCCTAGAAGTTGCTGCCAAGATTTTAATTGGGGAGGACCATGTGGAAGATTTGG ATAAAAGCAAGTTTAAAACAAAAATTAGGAGGTTGTATGATATAGCTAATGTTCTGAGTAGCCTGGATCT TATCAAGAAAGTTCATGTTACAGAGGAAAGAGGCCGAAAACCAGCTTTCAAATGGACCGGCCCAGAAATC AGTCCAAATACCAGTGGCTCCAGCCCAGTCATTCATTTTACTCCCTCTGATTTGGAGGTGAGACGGTCTT CAAAAGAGAACTGTGCCAAAAACCTCTTTTCCACACGTGGGAAACCAAACTTTACTCGACACCCATCTCT TATCAAATTGGTAAAGAGTATAGAAAGTGATCGGAGAAAGATAAATTCTGCGCCCAGTAGCCCTATCAAG ACCAACAAAGCTGAGAGTTCTCAGAATTCTGCACCCTTCCCAAGTAAAATGGCTCAGCTCGCAGCTATTT GTAAAATGCAGTTAGAAGAGCAATCAAGTGAATCCAGACAGAAAGTGAAAGTACAGCTGGCAAGATCTGG ACCCTGCAAACCAGTAGCCCCTCTGGACCCCCCAGTGAATGCTGAGATGGAGCTGACAGCACCGTCCCTC ATCCAGCCCCTGGGAATGGTTCCCCTGATCCCCAGCCCCTTGTCATCAGCAGTGCCCCTGATCCTACCTC AGGCCCCTTCAGGCCCATCCTATGCCATCTACCTGCAGCCCACTCAAGCCCACCAAAGTGTGACCCCACC CCAAGGCCTGAGCCCAACGGTGTGCACCACCCACTCTTCTAAAGCTACTGGCTCAAAAGACTCCACAGAT GCCACCACTGAGAAGGCAGCCAATGATACCTCAAAGGCCAGTGCCTCTACCAGGCCTGGAAGCTTGCTGC CAGCACCAGAGAGGCAAGGGGCAAAGAGCCGAACCAGGGAGCCAGCTGGAGAAAGAGGCTCAAAGAGGGC AAGCATGCTCGAGGACAGTGGTTCCAAAAAGAAATTTAAAGAGGACCTAAAAGGACTTGAAAATGTCTCC GCAACCTTGTTCCCATCAGGATACCTAATCCCTCTCACGCAGTGCTCATCCCTGGGGGCAGAGTCCATTT TGTCTGGTAAAGAAAACTCAAGTGCTCTTTCCCCAAACCACAGGATTTACAGCTCCCCAATTGCAGGTGT TATTCCAGTGACATCATCTGAACTCACTGCTGTTAATTTTCCCTCTTTTCATGTAACACCGTTGAAGCTA ATGGTCTCACCAACTTCCGTGGCAGCCGTACCTGTCGGGAACAGCCCGGCTCTCGCTTCAAGCCACCCTG TTCCCATCCAGAACCCAAGCTCAGCCATTGTAAACTTCACCCTGCAGCACCTGGGACTCATCTCACCCAA TGTGCAGTTGTCTGCCAGCCCTGGGTCTGGAATCGTTCCTGTGTCTCCAAGAATAGAGTCTGTTAATGTC GCACCAGAAAATGCAGGCACTCAGCAAGGAAGGGCCACCAACTATGACTCACCAGTCCCAGGCCAGAGCC AGCCAAATGGACAATCAGTTGCTGTGACAGGGGCACAACAGCCTGTTCCTGTGACACCCAAAGGGTCACA ATTAGTGGCCGAAAGTTTCTTCCGTACCCCAGGTGGACCCACCAAGCCAACCAGCTCATCCTGCATGGAT TTTGAGGGTGCTAATAAAACCTCCTTAGGAACTCTCTTTGTCCCACAGCGAAAACTGGAAGTCTCAACAG AGGATGTCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213597 protein sequence
Red=Cloning site Green=Tags(s) MENEKENLFCEPHKRGLMKTPLKESTTANIVLAEIQPDFGPLTTPTKPKEGSQGEPWTPTANLKMLISAV SPEIRNRDQKRGLFDNRSGLPEAKDCIHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNND ICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLNKTLGTLKSIGEENKYAEQIMMIK KKEYEQEFDFIKSYSIEDHIIKSNTGPNGHPDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVS TPQIVSLEVAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEI SPNTSGSSPVIHFTPSDLEVRRSSKENCAKNLFSTRGKPNFTRHPSLIKLVKSIESDRRKINSAPSSPIK TNKAESSQNSAPFPSKMAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSL IQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTD ATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVS ATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKL MVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNV APENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMD FEGANKTSLGTLFVPQRKLEVSTEDVH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024680 |
ORF Size | 2601 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024680.2, NM_024680.3, NP_078956.2 |
RefSeq Size | 3736 bp |
RefSeq ORF | 2604 bp |
Locus ID | 79733 |
Protein Families | Druggable Genome |
MW | 94.2 kDa |
Gene Summary | This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC305136 | E2F8 (untagged)-Human E2F transcription factor 8 (E2F8) |
USD 760.00 |
|
RG213597 | E2F8 (GFP-tagged) - Human E2F transcription factor 8 (E2F8) |
USD 830.00 |
|
RC213597L3 | Lenti ORF clone of Human E2F transcription factor 8 (E2F8), Myc-DDK-tagged |
USD 1,176.00 |
|
RC213597L4 | Lenti ORF clone of Human E2F transcription factor 8 (E2F8), mGFP tagged |
USD 950.00 |
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