E2F8 (NM_024680) Human Tagged ORF Clone

CAT#: RC213597

E2F8 (Myc-DDK-tagged)-Human E2F transcription factor 8 (E2F8)

Product Images

Western blot validation of overexpression lysate (Cat# LY411140) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213597 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2F8
• Synonyms: E2F-8
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213597 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAACGAAAAGGAAAATCTCTTTTGTGAGCCACATAAAAGGGGACTAATGAAAACACCTCTGAAAG
  AATCCACCACAGCAAATATCGTGTTGGCAGAGATCCAGCCTGACTTTGGCCCTTTAACCACACCTACCAA
  GCCCAAGGAAGGCTCTCAGGGAGAGCCGTGGACACCGACAGCCAACCTGAAAATGCTCATCAGTGCTGTG
  AGCCCTGAGATCCGCAACAGAGATCAGAAAAGGGGTTTGTTTGACAACAGAAGTGGATTACCTGAGGCCA
  AAGACTGTATACACGAACACTTATCTGGAGATGAATTTGAGAAATCCCAACCAAGTCGAAAAGAGAAAAG
  TTTAGGATTATTGTGTCATAAGTTCTTAGCACGATATCCTAATTATCCCAACCCTGCTGTGAATAATGAC
  ATCTGCCTTGACGAAGTGGCAGAGGAACTTAATGTTGAACGTCGACGCATTTACGATATCGTGAACGTCC
  TAGAGAGTTTACATATGGTGAGCCGCCTCGCCAAAAACAGGTACACTTGGCACGGGCGACACAATCTCAA
  CAAAACCCTTGGCACCTTGAAGAGCATCGGGGAGGAGAATAAGTACGCCGAGCAGATTATGATGATCAAA
  AAGAAAGAATATGAGCAAGAGTTTGACTTTATTAAGAGTTACAGTATAGAGGATCATATCATCAAATCAA
  ACACTGGCCCAAATGGACACCCAGACATGTGTTTTGTGGAACTCCCTGGAGTGGAATTTCGGGCAGCTTC
  TGTAAACAGCCGCAAAGACAAGTCTTTAAGGGTAATGAGCCAGAAATTTGTGATGCTGTTTTTGGTGTCA
  ACGCCTCAGATAGTAAGCCTAGAAGTTGCTGCCAAGATTTTAATTGGGGAGGACCATGTGGAAGATTTGG
  ATAAAAGCAAGTTTAAAACAAAAATTAGGAGGTTGTATGATATAGCTAATGTTCTGAGTAGCCTGGATCT
  TATCAAGAAAGTTCATGTTACAGAGGAAAGAGGCCGAAAACCAGCTTTCAAATGGACCGGCCCAGAAATC
  AGTCCAAATACCAGTGGCTCCAGCCCAGTCATTCATTTTACTCCCTCTGATTTGGAGGTGAGACGGTCTT
  CAAAAGAGAACTGTGCCAAAAACCTCTTTTCCACACGTGGGAAACCAAACTTTACTCGACACCCATCTCT
  TATCAAATTGGTAAAGAGTATAGAAAGTGATCGGAGAAAGATAAATTCTGCGCCCAGTAGCCCTATCAAG
  ACCAACAAAGCTGAGAGTTCTCAGAATTCTGCACCCTTCCCAAGTAAAATGGCTCAGCTCGCAGCTATTT
  GTAAAATGCAGTTAGAAGAGCAATCAAGTGAATCCAGACAGAAAGTGAAAGTACAGCTGGCAAGATCTGG
  ACCCTGCAAACCAGTAGCCCCTCTGGACCCCCCAGTGAATGCTGAGATGGAGCTGACAGCACCGTCCCTC
  ATCCAGCCCCTGGGAATGGTTCCCCTGATCCCCAGCCCCTTGTCATCAGCAGTGCCCCTGATCCTACCTC
  AGGCCCCTTCAGGCCCATCCTATGCCATCTACCTGCAGCCCACTCAAGCCCACCAAAGTGTGACCCCACC
  CCAAGGCCTGAGCCCAACGGTGTGCACCACCCACTCTTCTAAAGCTACTGGCTCAAAAGACTCCACAGAT
  GCCACCACTGAGAAGGCAGCCAATGATACCTCAAAGGCCAGTGCCTCTACCAGGCCTGGAAGCTTGCTGC
  CAGCACCAGAGAGGCAAGGGGCAAAGAGCCGAACCAGGGAGCCAGCTGGAGAAAGAGGCTCAAAGAGGGC
  AAGCATGCTCGAGGACAGTGGTTCCAAAAAGAAATTTAAAGAGGACCTAAAAGGACTTGAAAATGTCTCC
  GCAACCTTGTTCCCATCAGGATACCTAATCCCTCTCACGCAGTGCTCATCCCTGGGGGCAGAGTCCATTT
  TGTCTGGTAAAGAAAACTCAAGTGCTCTTTCCCCAAACCACAGGATTTACAGCTCCCCAATTGCAGGTGT
  TATTCCAGTGACATCATCTGAACTCACTGCTGTTAATTTTCCCTCTTTTCATGTAACACCGTTGAAGCTA
  ATGGTCTCACCAACTTCCGTGGCAGCCGTACCTGTCGGGAACAGCCCGGCTCTCGCTTCAAGCCACCCTG
  TTCCCATCCAGAACCCAAGCTCAGCCATTGTAAACTTCACCCTGCAGCACCTGGGACTCATCTCACCCAA
  TGTGCAGTTGTCTGCCAGCCCTGGGTCTGGAATCGTTCCTGTGTCTCCAAGAATAGAGTCTGTTAATGTC
  GCACCAGAAAATGCAGGCACTCAGCAAGGAAGGGCCACCAACTATGACTCACCAGTCCCAGGCCAGAGCC
  AGCCAAATGGACAATCAGTTGCTGTGACAGGGGCACAACAGCCTGTTCCTGTGACACCCAAAGGGTCACA
  ATTAGTGGCCGAAAGTTTCTTCCGTACCCCAGGTGGACCCACCAAGCCAACCAGCTCATCCTGCATGGAT
  TTTGAGGGTGCTAATAAAACCTCCTTAGGAACTCTCTTTGTCCCACAGCGAAAACTGGAAGTCTCAACAG
  AGGATGTCCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213597 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MENEKENLFCEPHKRGLMKTPLKESTTANIVLAEIQPDFGPLTTPTKPKEGSQGEPWTPTANLKMLISAV
  SPEIRNRDQKRGLFDNRSGLPEAKDCIHEHLSGDEFEKSQPSRKEKSLGLLCHKFLARYPNYPNPAVNND
  ICLDEVAEELNVERRRIYDIVNVLESLHMVSRLAKNRYTWHGRHNLNKTLGTLKSIGEENKYAEQIMMIK
  KKEYEQEFDFIKSYSIEDHIIKSNTGPNGHPDMCFVELPGVEFRAASVNSRKDKSLRVMSQKFVMLFLVS
  TPQIVSLEVAAKILIGEDHVEDLDKSKFKTKIRRLYDIANVLSSLDLIKKVHVTEERGRKPAFKWTGPEI
  SPNTSGSSPVIHFTPSDLEVRRSSKENCAKNLFSTRGKPNFTRHPSLIKLVKSIESDRRKINSAPSSPIK
  TNKAESSQNSAPFPSKMAQLAAICKMQLEEQSSESRQKVKVQLARSGPCKPVAPLDPPVNAEMELTAPSL
  IQPLGMVPLIPSPLSSAVPLILPQAPSGPSYAIYLQPTQAHQSVTPPQGLSPTVCTTHSSKATGSKDSTD
  ATTEKAANDTSKASASTRPGSLLPAPERQGAKSRTREPAGERGSKRASMLEDSGSKKKFKEDLKGLENVS
  ATLFPSGYLIPLTQCSSLGAESILSGKENSSALSPNHRIYSSPIAGVIPVTSSELTAVNFPSFHVTPLKL
  MVSPTSVAAVPVGNSPALASSHPVPIQNPSSAIVNFTLQHLGLISPNVQLSASPGSGIVPVSPRIESVNV
  APENAGTQQGRATNYDSPVPGQSQPNGQSVAVTGAQQPVPVTPKGSQLVAESFFRTPGGPTKPTSSSCMD
  FEGANKTSLGTLFVPQRKLEVSTEDVH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024680
• ORF Size: 2601 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024680.2, NM_024680.3, NP_078956.2
• RefSeq Size: 3736 bp
• RefSeq ORF: 2604 bp
• Locus ID: 79733
• Protein Families: Druggable Genome
• MW: 94.2 kDa
• Gene Summary: This gene encodes a member of a family of transcription factors which regulate the expression of genes required for progression through the cell cycle. The encoded protein regulates progression from G1 to S phase by ensuring the nucleus divides at the proper time. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012]