SEZ6L (NM_021115) Human Tagged ORF Clone

CAT#: RC213624

SEZ6L (Myc-DDK-tagged)-Human seizure related 6 homolog (mouse)-like (SEZ6L), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY433621) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC230622 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified SEZ6L protein (Cat# TP313624). The protein was produced from HEK293T cells transfected with SEZ6L cDNA clone (Cat# RC213624) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SEZ6L
• Synonyms: Acig1; acupuncture induced gene 1; AI843918; AIG1; AW539724; BSRP-B; mKIAA0927; seizure related 6 homolog like
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213624 representing NM_021115
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCGCGGCCCGGCCGCCCGCCGCGGGACTCCGCGGGATCTCGCTGTTCCTCGCTCTGCTCCTGGGGA
  GCCCGGCGGCAGCGCTGGAGCGAGATGCTCTTCCCGAGGGAGATGCTAGCCCTTTGGGTCCTTACCTCCT
  GCCCTCAGGAGCCCCGGAGAGAGGCAGTCCTGGCAAAGAGCACCCTGAAGAGAGAGTGGTAACAGCGCCC
  CCCAGTTCCTCACAGTCGGCGGAAGTGCTGGGCGAGCTGGTGCTGGATGGGACCGCACCCTCTGCACATC
  ACGACATCCCAGCCCTGTCACCGCTGCTTCCAGAGGAGGCCCGCCCCAAGCACGCCTTGCCCCCCAAGAA
  GAAACTGCCTTCGCTCAAGCAGGTGAACTCTGCCAGGAAGCAGCTGAGGCCCAAGGCCACCTCCGCAGCC
  ACTGTCCAAAGGGCAGGGTCCCAGCCAGCGTCCCAGGGCCTAGATCTCCTCTCCTCCTCCACGGAGAAGC
  CTGGCCCACCGGGGGACCCGGACCCCATCGTGGCCTCCGAGGAGGCATCAGAAGTGCCCCTTTGGCTGGA
  CCGAAAGGAGAGTGCGGTCCCTACAACACCCGCACCCCTGCAAATCTCCCCCTTCACTTCGCAGCCCTAT
  GTGGCCCACACACTCCCCCAGAGGCCAGAACCCGGGGAGCCTGGGCCTGACATGGCCCAGGAGGCCCCCC
  AGGAGGACACCAGCCCCATGGCCCTGATGGACAAAGGTGAGAATGAGCTGACTGGGTCAGCCTCAGAGGA
  GAGCCAGGAGACCACTACCTCCACCATTATCACCACCACGGTCATCACCACCGAGCAGGCACCAGCTCTC
  TGCAGTGTGAGCTTCTCCAATCCTGAGGGGTACATTGACTCCAGCGACTACCCACTGCTGCCCCTCAACA
  ACTTTCTGGAGTGCACATACAACGTGACAGTCTACACTGGCTATGGGGTGGAGCTCCAGGTGAAGAGTGT
  GAACCTGTCCGATGGGGAACTGCTCTCCATCCGCGGGGTGGACGGCCCTACCCTGACCGTCCTGGCCAAC
  CAGACACTCCTGGTGGAGGGGCAGGTAATCCGAAGCCCCACCAACACCATCTCCGTCTACTTCCGGACCT
  TCCAGGACGACGGCCTTGGGACCTTCCAGCTTCACTACCAGGCCTTCATGCTGAGCTGCAACTTTCCCCG
  CCGGCCTGACTCTGGGGATGTCACGGTGATGGACCTGCACTCAGGTGGGGTGGCCCACTTTCACTGCCAC
  CTGGGCTATGAGCTCCAGGGCGCTAAGATGCTGACATGCATCAATGCCTCCAAGCCGCACTGGAGCAGCC
  AGGAGCCCATCTGCTCAGCTCCTTGTGGAGGGGCAGTGCACAATGCCACCATCGGCCGCGTCCTCTCCCC
  AAGTTACCCTGAAAACACAAATGGGAGCCAATTCTGCATCTGGACGATTGAAGCTCCAGAGGGCCAGAAG
  CTGCACCTGCACTTTGAGAGGCTGTTGCTGCATGACAAGGACAGGATGACGGTTCACAGCGGGCAGACCA
  ACAAGTCAGCTCTTCTCTACGACTCCCTTCAAACCGAGAGTGTCCCTTTTGAGGGCCTGCTGAGCGAAGG
  CAACACCATCCGCATCGAGTTCACGTCCGACCAGGCCCGGGCGGCCTCCACCTTCAACATCCGATTTGAA
  GCGTTTGAGAAAGGCCACTGCTATGAGCCCTACATCCAGAATGGGAACTTCACTACATCCGACCCGACCT
  ATAACATTGGGACTATAGTGGAGTTCACCTGCGACCCCGGCCACTCCCTGGAGCAGGGCCCGGCCATCAT
  CGAATGCATCAATGTGCGGGACCCATACTGGAATGACACAGAGCCCCTGTGCAGAGCCATGTGTGGTGGG
  GAGCTCTCTGCTGTGGCTGGGGTGGTATTGTCCCCAAACTGGCCCGAGCCCTACGTGGAAGGTGAAGATT
  GTATCTGGAAGATCCACGTGGGAGAAGAGAAACGGATCTTCTTAGATATCCAGTTCCTGAATCTGAGCAA
  CAGTGACATCTTGACCATCTACGATGGCGACGAGGTCATGCCCCACATCTTGGGGCAGTACCTTGGGAAC
  AGTGGCCCCCAGAAACTGTACTCCTCCACGCCAGACTTAACCATCCAGTTCCATTCGGACCCTGCTGGCC
  TCATCTTTGGAAAGGGCCAGGGATTTATCATGAACTACATAGAGGTATCAAGGAATGACTCCTGCTCGGA
  TTTACCCGAGATCCAGAATGGCTGGAAAACCACTTCTCACACGGAGTTGGTGCGGGGAGCCAGAATCACC
  TACCAGTGTGACCCCGGCTATGACATCGTGGGGAGTGACACCCTCACCTGCCAGTGGGACCTCAGCTGGA
  GCAGCGACCCCCCATTTTGTGAGAAAATTATGTACTGCACCGACCCCGGAGAGGTGGATCACTCGACCCG
  CTTAATTTCGGATCCTGTGCTGCTGGTGGGGACCACCATCCAATACACCTGCAACCCCGGTTTTGTGCTT
  GAAGGGAGTTCTCTTCTGACCTGCTACAGCCGTGAAACAGGGACTCCCATCTGGACGTCTCGCCTGCCCC
  ACTGCGTTTCGGAGGAGTCCCTGGCATGTGACAACCCAGGGCTGCCTGAAAATGGATACCAAATCCTGTA
  CAAGCGACTCTACCTGCCAGGAGAGTCCCTCACCTTCATGTGCTACGAAGGCTTTGAGCTCATGGGTGAA
  GTGACCATCCGCTGCATCCTGGGACAGCCATCCCACTGGAACGGGCCCCTGCCCGTGTGTAAAGTTAATC
  AAGACAGTTTTGAACATGCTTTAGAAGTAGCAGAAGCGGCAGCAGAGACGTCGCTGGAAGGGGGGAACAT
  GGCCCTGGCTATCTTCATCCCGGTCCTCATCATCTCCTTACTGCTGGGAGGAGCCTACATTTACATCACA
  AGATGTCGCTACTATTCCAACCTCCGCCTGCCTCTGATGTACTCCCACCCCTACAGCCAGATCACCGTGG
  AAACCGAGTTTGACAACCCCATTTACGAGACAGGGGAAACCAGAGAGTATGAGGTTTCTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213624 representing NM_021115
  Red=Cloning site Green=Tags(s)
  
  MPAARPPAAGLRGISLFLALLLGSPAAALERDALPEGDASPLGPYLLPSGAPERGSPGKEHPEERVVTAP
  PSSSQSAEVLGELVLDGTAPSAHHDIPALSPLLPEEARPKHALPPKKKLPSLKQVNSARKQLRPKATSAA
  TVQRAGSQPASQGLDLLSSSTEKPGPPGDPDPIVASEEASEVPLWLDRKESAVPTTPAPLQISPFTSQPY
  VAHTLPQRPEPGEPGPDMAQEAPQEDTSPMALMDKGENELTGSASEESQETTTSTIITTTVITTEQAPAL
  CSVSFSNPEGYIDSSDYPLLPLNNFLECTYNVTVYTGYGVELQVKSVNLSDGELLSIRGVDGPTLTVLAN
  QTLLVEGQVIRSPTNTISVYFRTFQDDGLGTFQLHYQAFMLSCNFPRRPDSGDVTVMDLHSGGVAHFHCH
  LGYELQGAKMLTCINASKPHWSSQEPICSAPCGGAVHNATIGRVLSPSYPENTNGSQFCIWTIEAPEGQK
  LHLHFERLLLHDKDRMTVHSGQTNKSALLYDSLQTESVPFEGLLSEGNTIRIEFTSDQARAASTFNIRFE
  AFEKGHCYEPYIQNGNFTTSDPTYNIGTIVEFTCDPGHSLEQGPAIIECINVRDPYWNDTEPLCRAMCGG
  ELSAVAGVVLSPNWPEPYVEGEDCIWKIHVGEEKRIFLDIQFLNLSNSDILTIYDGDEVMPHILGQYLGN
  SGPQKLYSSTPDLTIQFHSDPAGLIFGKGQGFIMNYIEVSRNDSCSDLPEIQNGWKTTSHTELVRGARIT
  YQCDPGYDIVGSDTLTCQWDLSWSSDPPFCEKIMYCTDPGEVDHSTRLISDPVLLVGTTIQYTCNPGFVL
  EGSSLLTCYSRETGTPIWTSRLPHCVSEESLACDNPGLPENGYQILYKRLYLPGESLTFMCYEGFELMGE
  VTIRCILGQPSHWNGPLPVCKVNQDSFEHALEVAEAAAETSLEGGNMALAIFIPVLIISLLLGGAYIYIT
  RCRYYSNLRLPLMYSHPYSQITVETEFDNPIYETGETREYEVSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021115
• ORF Size: 3072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021115.2, NM_021115.3, NM_021115.4, NP_066938.2
• RefSeq Size: 3424
• RefSeq ORF: 3075
• Locus ID: 23544
• Domains: CCP, CUB
• Protein Families: Druggable Genome, Transmembrane
• MW: 109 kDa