UBE2Q1 (NM_017582) Human Tagged ORF Clone
CAT#: RC213678
UBE2Q1 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1)
"NM_017582" in other vectors (5)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | UBE2Q1 |
Synonyms | GTAP; NICE-5; NICE5; PRO3094; UBE2Q |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC213678 representing NM_017582
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCAGCCGCAGCCGCAGGGGCAGCAGCAGCCGGGGCCGGGGCAGCAGCTGGGGGGCCAGGGGGCGG CGCCGGGGGCCGGGGGCGGCCCAGGGGGGGGCCCGGGGCCGGGGCCCTGCCTGAGGCGAGAGCTGAAGCT GCTCGAGTCCATCTTCCACCGCGGCCACGAGCGCTTCCGCATTGCCAGCGCCTGCCTGGACGAGCTGAGC TGCGAGTTCCTGCTGGCTGGGGCCGGAGGGGCCGGGGCGGGGGCCGCGCCCGGACCGCATCTCCCCCCAC GGGGGTCGGTGCCTGGGGATCCTGTCCGCATCCACTGCAACATCACGGAGTCATACCCTGCTGTGCCCCC CATCTGGTCGGTGGAGTCTGATGACCCTAACTTGGCTGCTGTCTTGGAGAGGCTGGTGGACATAAAGAAA GGGAATACTCTGCTATTGCAGCATCTGAAGAGGATCATCTCCGACCTGTGTAAACTCTATAACCTCCCTC AGCATCCAGATGTGGAGATGCTGGATCAACCCTTGCCAGCAGAGCAGTGCACACAGGAAGACGTGTCTTC AGAAGATGAAGATGAGGAGATGCCTGAGGACACAGAAGACTTAGATCACTATGAAATGAAAGAGGAAGAG CCAGCTGAGGGCAAGAAATCTGAAGATGATGGCATTGGAAAAGAAAACTTGGCCATCCTAGAGAAAATTA AAAAGAACCAGAGGCAAGATTACTTAAATGGTGCAGTGTCTGGCTCGGTGCAGGCCACTGACCGGCTGAT GAAGGAGCTCAGGGATATATACCGATCACAGAGTTTCAAAGGCGGAAACTATGCAGTCGAACTCGTGAAT GACAGTCTGTATGATTGGAATGTCAAACTCCTCAAAGTTGACCAGGACAGCGCTTTGCACAACGATCTCC AGATCCTCAAAGAGAAAGAAGGAGCCGACTTCATTCTACTTAACTTTTCCTTTAAAGATAACTTTCCCTT TGACCCACCATTTGTCAGGGTTGTGTCTCCAGTCCTCTCTGGAGGGTATGTTCTGGGCGGAGGGGCCATC TGCATGGAACTTCTCACCAAACAGGGCTGGAGCAGTGCCTACTCCATAGAGTCAGTGATCATGCAGATCA GTGCCACACTGGTGAAGGGGAAAGCACGAGTGCAGTTTGGAGCCAACAAATCTCAATACAGTCTGACAAG AGCACAGCAGTCCTACAAGTCCTTGGTGCAGATCCACGAAAAAAACGGCTGGTACACACCCCCAAAAGAA GACGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC213678 representing NM_017582
Red=Cloning site Green=Tags(s) MQQPQPQGQQQPGPGQQLGGQGAAPGAGGGPGGGPGPGPCLRRELKLLESIFHRGHERFRIASACLDELS CEFLLAGAGGAGAGAAPGPHLPPRGSVPGDPVRIHCNITESYPAVPPIWSVESDDPNLAAVLERLVDIKK GNTLLLQHLKRIISDLCKLYNLPQHPDVEMLDQPLPAEQCTQEDVSSEDEDEEMPEDTEDLDHYEMKEEE PAEGKKSEDDGIGKENLAILEKIKKNQRQDYLNGAVSGSVQATDRLMKELRDIYRSQSFKGGNYAVELVN DSLYDWNVKLLKVDQDSALHNDLQILKEKEGADFILLNFSFKDNFPFDPPFVRVVSPVLSGGYVLGGGAI CMELLTKQGWSSAYSIESVIMQISATLVKGKARVQFGANKSQYSLTRAQQSYKSLVQIHEKNGWYTPPKE DG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_017582 |
ORF Size | 1266 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017582.4, NM_017582.5, NM_017582.6, NP_060052.3 |
RefSeq Size | 3223 bp |
RefSeq ORF | 1269 bp |
Locus ID | 55585 |
Domains | UBCc |
Protein Pathways | Ubiquitin mediated proteolysis |
MW | 45.9 kDa |
Gene Summary | The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is 98% identical to the mouse counterpart. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC114081 | UBE2Q1 (untagged)-Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) |
USD 710.00 |
|
SC317683 | UBE2Q1 (untagged)-Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) |
USD 710.00 |
|
RG213678 | UBE2Q1 (GFP-tagged) - Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1) |
USD 460.00 |
|
RC213678L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1), Myc-DDK-tagged |
USD 620.00 |
|
RC213678L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2Q family member 1 (UBE2Q1), mGFP tagged |
USD 620.00 |
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