CTRP3 (C1QTNF3) (NM_181435) Human Tagged ORF Clone

CAT#: RC213742

C1QTNF3 (Myc-DDK-tagged)-Human C1q and tumor necrosis factor related protein 3 (C1QTNF3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405756) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213742 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified C1QTNF3 protein (Cat# TP313742). The protein was produced from HEK293T cells transfected with C1QTNF3 cDNA clone (Cat# RC213742) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: C1QTNF3
• Synonyms: C1ATNF3; CORCS; CORS; CORS-26; CORS26; CTRP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213742 representing NM_181435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTTTGGAGGCAGCTCATCTATTGGCAACTGCTGGCTTTGTTTTTCCTCCCTTTTTGCCTGTGTCAAG
  ATGAATACATGGAGGTGAGCGGAAGAACTAATAAAGTGGTGGCAAGAATAGTGCAAAGCCACCAGCAGAC
  TGGCCGTAGCGGCTCCAGGAGGGAGAAAGTGAGAGAGCGGAGCCATCCTAAAACTGGGACTGTGGATAAT
  AACACTTCTACAGACCTAAAATCCCTGAGACCAGATGAGCTACCGCACCCCGAGGTAGATGACCTAGCCC
  AGATCACCACATTCTGGGGCCAGTCTCCACAAACCGGAGGACTACCCCCAGACTGCAGTAAGTGTTGTCA
  TGGAGACTACAGCTTTCGAGGCTACCAAGGCCCCCCTGGGCCACCGGGCCCTCCTGGCATTCCAGGAAAC
  CATGGAAACAATGGCAACAATGGAGCCACTGGTCATGAAGGAGCCAAAGGTGAGAAGGGCGACAAAGGTG
  ACCTGGGGCCTCGAGGGGAGCGGGGGCAGCATGGCCCCAAAGGAGAGAAGGGCTACCCGGGGATTCCACC
  AGAACTTCAGATTGCATTCATGGCTTCTCTGGCAACCCACTTCAGCAATCAGAACAGTGGGATTATCTTC
  AGCAGTGTTGAGACCAACATTGGAAACTTCTTTGATGTCATGACTGGTAGATTTGGGGCCCCAGTATCAG
  GTGTGTATTTCTTCACCTTCAGCATGATGAAGCATGAGGATGTTGAGGAAGTGTATGTGTACCTTATGCA
  CAATGGCAACACAGTCTTCAGCATGTACAGCTATGAAATGAAGGGCAAATCAGATACATCCAGCAATCAT
  GCTGTGCTGAAGCTAGCCAAAGGGGATGAGGTTTGGCTGCGAATGGGCAATGGCGCTCTCCATGGGGACC
  ACCAACGCTTCTCCACCTTTGCAGGATTCCTGCTCTTTGAAACTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213742 representing NM_181435
  Red=Cloning site Green=Tags(s)
  
  MLWRQLIYWQLLALFFLPFCLCQDEYMEVSGRTNKVVARIVQSHQQTGRSGSRREKVRERSHPKTGTVDN
  NTSTDLKSLRPDELPHPEVDDLAQITTFWGQSPQTGGLPPDCSKCCHGDYSFRGYQGPPGPPGPPGIPGN
  HGNNGNNGATGHEGAKGEKGDKGDLGPRGERGQHGPKGEKGYPGIPPELQIAFMASLATHFSNQNSGIIF
  SSVETNIGNFFDVMTGRFGAPVSGVYFFTFSMMKHEDVEEVYVYLMHNGNTVFSMYSYEMKGKSDTSSNH
  AVLKLAKGDEVWLRMGNGALHGDHQRFSTFAGFLLFETK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_181435
• ORF Size: 957 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181435.4, NM_181435.5, NP_852100.3
• RefSeq Size: 3789 bp
• RefSeq ORF: 960 bp
• Locus ID: 114899
• Protein Families: Secreted Protein
• MW: 35 kDa