P2Y4 (P2RY4) (NM_002565) Human Tagged ORF Clone

CAT#: RC213891

P2RY4 (Myc-DDK-tagged)-Human pyrimidinergic receptor P2Y, G-protein coupled, 4 (P2RY4)

Product Images

Western blot validation of overexpression lysate (Cat# LY400911) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213891 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2RY4
• Synonyms: NRU; P2P; P2Y4; UNR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213891 representing NM_002565
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGTACAGAGTCCTCCCTGTTGAGATCCCTAGGCCTCAGCCCAGGTCCTGGCAGCAGTGAGGTGG
  AGCTGGACTGTTGGTTTGATGAGGATTTCAAGTTCATCCTGCTGCCTGTGAGCTATGCAGTTGTCTTTGT
  GCTGGGCTTGGGCCTTAACGCCCCAACCCTATGGCTCTTCATCTTCCGCCTCCGACCCTGGGATGCAACG
  GCCACCTACATGTTCCACCTGGCATTGTCAGACACCTTGTATGTGCTGTCGCTGCCCACCCTCATCTACT
  ATTATGCAGCCCACAACCACTGGCCCTTTGGCACTGAGATCTGCAAGTTCGTCCGCTTTCTTTTCTATTG
  GAACCTCTACTGCAGTGTCCTTTTCCTCACCTGCATCAGCGTGCACCGCTACCTGGGCATCTGCCACCCA
  CTTCGGGCACTACGCTGGGGCCGCCCTCGCCTCGCAGGCCTTCTCTGCCTGGCAGTTTGGTTGGTCGTAG
  CCGGCTGCCTCGTGCCCAACCTGTTCTTTGTCACAACCAGCAACAAAGGGACCACCGTCCTGTGCCATGA
  CACCACTCGGCCTGAAGAGTTTGACCACTATGTGCACTTCAGCTCGGCGGTCATGGGGCTGCTCTTTGGC
  GTGCCCTGCCTGGTCACTCTTGTTTGCTATGGACTCATGGCTCGTCGCCTGTATCAGCCCTTGCCAGGCT
  CTGCACAGTCGTCTTCTCGCCTCCGCTCTCTCCGCACCATAGCTGTGGTGCTGACTGTCTTTGCTGTCTG
  CTTCGTGCCTTTCCACATCACCCGCACCATTTACTACCTGGCCAGGCTGTTGGAAGCTGACTGCCGAGTA
  CTGAACATTGTCAACGTGGTCTATAAAGTGACTCGGCCCCTGGCCAGTGCCAACAGCTGCCTGGATCCTG
  TGCTCTACTTGCTCACTGGGGACAAATATCGACGTCAGCTCCGTCAGCTCTGTGGTGGTGGCAAGCCCCA
  GCCCCGCACGGCTGCCTCTTCCCTGGCACTAGTGTCCCTGCCTGAGGATAGCAGCTGCAGGTGGGCGGCC
  ACCCCCCAGGACAGTAGCTGCTCTACTCCTAGGGCAGATAGATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213891 representing NM_002565
  Red=Cloning site Green=Tags(s)
  
  MASTESSLLRSLGLSPGPGSSEVELDCWFDEDFKFILLPVSYAVVFVLGLGLNAPTLWLFIFRLRPWDAT
  ATYMFHLALSDTLYVLSLPTLIYYYAAHNHWPFGTEICKFVRFLFYWNLYCSVLFLTCISVHRYLGICHP
  LRALRWGRPRLAGLLCLAVWLVVAGCLVPNLFFVTTSNKGTTVLCHDTTRPEEFDHYVHFSSAVMGLLFG
  VPCLVTLVCYGLMARRLYQPLPGSAQSSSRLRSLRTIAVVLTVFAVCFVPFHITRTIYYLARLLEADCRV
  LNIVNVVYKVTRPLASANSCLDPVLYLLTGDKYRRQLRQLCGGGKPQPRTAASSLALVSLPEDSSCRWAA
  TPQDSSCSTPRADRL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002565
• ORF Size: 1095 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002565.1, NM_002565.2, NM_002565.3, NP_002556.1
• RefSeq Size: 1639 bp
• RefSeq ORF: 1098 bp
• Locus ID: 5030
• Protein Families: Druggable Genome
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 40.8 kDa
• Gene Summary: The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is responsive to uridine nucleotides, partially responsive to ATP, and not responsive to ADP. [provided by RefSeq, Jul 2008]