PAPOLG (NM_022894) Human Tagged ORF Clone

CAT#: RC213918

PAPOLG (Myc-DDK-tagged)-Human poly(A) polymerase gamma (PAPOLG)

Product Images

Western blot validation of overexpression lysate (Cat# LY411467) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213918 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PAPOLG
• Synonyms: FLJ11805; FLJ12972; FLJ13482; FLJ14187; MGC133307; MGC133308
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213918 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAGAGATGTCTGCAAACACCGTGCTGGACAGCCAGCGTCAACAAAAGCATTATGGAATTACCTCCC
  CAATTAGTTTGGCATCTCCTAAAGAAATTGATCATATTTACACACAGAAATTAATTGACGCCATGAAACC
  ATTTGGAGTGTTTGAAGATGAGGAAGAATTGAACCACAGGCTGGTGGTTCTTGGTAAATTGAACAATTTA
  GTAAAAGAATGGATTTCTGATGTCAGCGAGAGTAAGAACCTCCCACCTTCTGTTGTGGCTACTGTTGGTG
  GTAAAATTTTCACATTTGGATCCTATAGGCTTGGAGTACACACCAAAGGAGCTGACATTGATGCACTTTG
  TGTAGCTCCAAGACATGTGGAAAGATCTGATTTTTTTCAGTCTTTTTTTGAAAAATTGAAACATCAAGAT
  GGCATTAGAAACTTAAGAGCTGTAGAAGATGCCTTTGTACCTGTTATAAAATTTGAATTTGATGGTATTG
  AAATTGATCTAGTCTTTGCAAGACTGGCAATACAAACCATATCAGATAATTTAGATCTAAGAGACGACTC
  TCGCCTGAGAAGCCTTGATATAAGGTGTATTCGCAGCTTAAATGGTTGTAGAGTTACTGATGAAATTTTG
  CATTTAGTGCCAAATAAAGAAACTTTTAGACTCACCCTAAGAGCTGTCAAATTATGGGCAAAACGACGTG
  GTATTTATTCCAACATGCTAGGATTCCTTGGTGGTGTCTCCTGGGCAATGCTAGTTGCAAGAACTTGCCA
  ATTGTATCCAAATGCAGCAGCATCTACTTTAGTTCATAAGTTCTTTTTAGTTTTTTCCAAGTGGGAATGG
  CCAAATCCTGTGCTGCTGAAGCAACCAGAAGAAAGCAATTTGAATTTGCCTGTCTGGGATCCTCGGGTAA
  ATCCATCAGATAGGTATCATCTCATGCCCATAATCACCCCTGCCTACCCACAACAGAATTCTACGTATAA
  TGTGTCCACATCAACTCGAACAGTAATGGTAGAAGAATTTAAACAAGGTCTTGCAGTCACAGATGAAATT
  CTTCAAGGAAAGTCAGATTGGTCCAAACTACTTGAGCCACCGAATTTCTTTCAAAAGTATAGACATTATA
  TAGTATTGACTGCCAGCGCATCAACAGAAGAAAACCATCTAGAGTGGGTTGGATTAGTAGAATCTAAAAT
  CCGTGTACTTGTTGGAAACTTGGAACGGAATGAATTTATTACTCTTGCCCATGTGAATCCCCAGTCATTC
  CCAGGGAATAAGGAACATCATAAAGACAACAATTACGTATCAATGTGGTTCCTTGGGATAATTTTTCGGA
  GAGTAGAAAATGCAGAAAGTGTCAACATAGACTTGACATATGATATACAGTCATTTACTGATACAGTGTA
  CAGACAGGCAAACAATATAAATATGCTAAAGGAGGGAATGAAAATTGAAGCAACTCATGTAAAGAAAAAA
  CAACTTCACCACTACCTTCCTGCAGAAATTCTTCAAAAGAAGAAAAAGCAAAGTCTCTCTGATGTCAATC
  GAAGCTCGGGCGGACTTCAATCCAAAAGATTGTCTCTGGATAGCAGTTGTCTGGATAGCTCCAGAGACAC
  TGATAATGGAACACCTTTTAATTCTCCAGCGTCCAAGTCTGATAGCCCTTCTGTAGGAGAAACAGAAAGG
  AATAGTGCTGAGCCTGCTGCTGTAATTGTGGAGAAGCCACTGAGTGTACCACCAGCCCAAGGACTTTCCA
  TTCCAGTGATTGGCGCAAAAGTTGACTCTACAGTAAAAACTGTATCACCCCCCACTGTGTGTACCATTCC
  TACCGTAGTAGGACGAAATGTCATTCCTAGAATCACAACACCTCACAACCCTGCCCAGGGACAACCGCAT
  CTGAATGGAATGTCAAATATAACTAAGACTGTTACACCTAAGAGATCCCATTCCCCATCCATAGATGGGA
  CTCCTAAGAGGTTGAAAGACGTAGAAAAGTTTATTCGACTTGAATCAACATTTAAGGACCCCCGCACTGC
  TGAAGAAAGAAAAAGAAAATCAGTGGATGCCATTGGAGGAGAATCTATGCCTATTCCAACTATTGATACA
  TCACGCAAAAAGAGACTACCCAGTAAAGAACTACCAGATTCATCATCTCCAGTTCCAGCAAACAACATCC
  GTGTCATCAAAAATTCCATTCGACTGACCCTTAATCGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213918 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MKEMSANTVLDSQRQQKHYGITSPISLASPKEIDHIYTQKLIDAMKPFGVFEDEEELNHRLVVLGKLNNL
  VKEWISDVSESKNLPPSVVATVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVERSDFFQSFFEKLKHQD
  GIRNLRAVEDAFVPVIKFEFDGIEIDLVFARLAIQTISDNLDLRDDSRLRSLDIRCIRSLNGCRVTDEIL
  HLVPNKETFRLTLRAVKLWAKRRGIYSNMLGFLGGVSWAMLVARTCQLYPNAAASTLVHKFFLVFSKWEW
  PNPVLLKQPEESNLNLPVWDPRVNPSDRYHLMPIITPAYPQQNSTYNVSTSTRTVMVEEFKQGLAVTDEI
  LQGKSDWSKLLEPPNFFQKYRHYIVLTASASTEENHLEWVGLVESKIRVLVGNLERNEFITLAHVNPQSF
  PGNKEHHKDNNYVSMWFLGIIFRRVENAESVNIDLTYDIQSFTDTVYRQANNINMLKEGMKIEATHVKKK
  QLHHYLPAEILQKKKKQSLSDVNRSSGGLQSKRLSLDSSCLDSSRDTDNGTPFNSPASKSDSPSVGETER
  NSAEPAAVIVEKPLSVPPAQGLSIPVIGAKVDSTVKTVSPPTVCTIPTVVGRNVIPRITTPHNPAQGQPH
  LNGMSNITKTVTPKRSHSPSIDGTPKRLKDVEKFIRLESTFKDPRTAEERKRKSVDAIGGESMPIPTIDT
  SRKKRLPSKELPDSSSPVPANNIRVIKNSIRLTLNR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022894
• ORF Size: 2208 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022894.2, NM_022894.3, NP_075045.2
• RefSeq Size: 7386 bp
• RefSeq ORF: 2211 bp
• Locus ID: 64895
• Domains: NTP_transf_2, PAP_RNA-bind, PAP_central
• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation
• MW: 82.8 kDa
• Gene Summary: This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]