PAG608 (ZMAT3) (NM_152240) Human Tagged ORF Clone

CAT#: RC213950

ZMAT3 (Myc-DDK-tagged)-Human zinc finger, matrin-type 3 (ZMAT3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403457) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213950 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZMAT3
• Synonyms: PAG608; WIG-1; WIG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213950 representing NM_152240
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCCTCTTGCAACACGCCGTGCTTCCTCCACCTAAGCAGCCCTCACCCTCGCCTCCTATGTCAGTGG
  CCACCAGGTCTACAGGAACCTTGCAGCTTCCACCACAGAAGCCTTTTGGGCAGGAGGCTTCCTTGCCTCT
  TGCAGGGGAAGAAGAGTTATCGAAGGGAGGGGAGCAAGACTGTGCCCTGGAGGAGCTATGTAAGCCCCTG
  TACTGCAAACTCTGCAATGTCACCTTGAACTCTGCACAGCAAGCCCAGGCTCATTATCAGGGTAAAAATC
  ATGGTAAGAAACTCCGAAATTACTATGCAGCAAATAGCTGTCCTCCTCCTGCTAGAATGAGCAATGTGGT
  CGAGCCTGCAGCTACTCCAGTTGTTCCAGTCCCTCCGCAGATGGGCTCCTTTAAGCCAGGAGGCCGAGTG
  ATCCTGGCCACGGAGAATGATTACTGTAAGCTCTGTGATGCCTCCTTCAGTTCCCCAGCTGTGGCTCAGG
  CTCACTATCAAGGGAAGAATCATGCCAAGAGGCTGCGGCTGGCGGAAGCTCAGAGTAACTCATTCTCGGA
  ATCCTCAGAGCTGGGTCAACGGCGGGCCAGGAAAGAAGGGAATGAGTTTAAGATGATGCCTAACAGGAGA
  AATATGTATACAGTACAGAATAATTCAGGTCCTTACTTCAATCCCCGCTCTCGGCAGAGAATTCCACGTG
  ATCTGGCCATGTGTGTTACTCCAAGTGGCCAGTTTTACTGCTCAATGTGTAATGTTGGAGCTGGCGAAGA
  GATGGAATTCCGGCAGCATTTAGAGAGCAAGCAACATAAGAGCAAGGTGTCTGAACAGCGGTACAGGAAT
  GAGATGGAGAATCTGGGATATGTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213950 representing NM_152240
  Red=Cloning site Green=Tags(s)
  
  MILLQHAVLPPPKQPSPSPPMSVATRSTGTLQLPPQKPFGQEASLPLAGEEELSKGGEQDCALEELCKPL
  YCKLCNVTLNSAQQAQAHYQGKNHGKKLRNYYAANSCPPPARMSNVVEPAATPVVPVPPQMGSFKPGGRV
  ILATENDYCKLCDASFSSPAVAQAHYQGKNHAKRLRLAEAQSNSFSESSELGQRRARKEGNEFKMMPNRR
  NMYTVQNNSGPYFNPRSRQRIPRDLAMCVTPSGQFYCSMCNVGAGEEMEFRQHLESKQHKSKVSEQRYRN
  EMENLGYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152240
• ORF Size: 864 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_152240.1, NM_152240.2, NP_689426.1
• RefSeq Size: 2323
• RefSeq ORF: 867
• Locus ID: 64393
• Domains: zf-C2H2
• Protein Families: Transcription Factors
• Protein Pathways: p53 signaling pathway
• MW: 31.8 kDa
• Gene Summary: This gene encodes a protein containing three zinc finger domains and a nuclear localization signal. The mRNA and the protein of this gene are upregulated by wildtype p53 and overexpression of this gene inhibits tumor cell growth, suggesting that this gene may have a role in the p53-dependent growth regulatory pathway. Alternative splicing of this gene results in two transcript variants encoding two isoforms differing in only one amino acid. [provided by RefSeq, Jul 2008]