PPP2R5C (NM_002719) Human Tagged ORF Clone
CAT#: RC214000
PPP2R5C (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1
"NM_002719" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PPP2R5C |
Synonyms | B56G; B56gamma; PR61G |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214000 representing NM_002719
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGACATGTAATAAAGCGGGCAGCAGGATGGTGGTGGATGCGGCCAACTCCAATGGGCCTTTCCAGC CCGTGGTCCTTCTCCATATTCGAGATGTTCCTCCTGCTGATCAAGAGAAGCTTTTTATCCAGAAGTTACG TCAGTGTTGCGTCCTCTTTGACTTTGTTTCTGATCCACTAAGTGACCTAAAGTGGAAGGAAGTAAAACGA GCTGCTTTAAGTGAAATGGTAGAATATATCACCCATAATCGGAATGTGATCACAGAGCCTATTTACCCAG AAGTAGTCCATATGTTTGCAGTTAACATGTTTCGAACATTACCACCTTCCTCCAATCCTACGGGAGCGGA ATTTGACCCGGAGGAAGATGAACCAACGTTAGAAGCAGCCTGGCCTCATCTACAGCTTGTTTATGAATTT TTCTTAAGATTTTTAGAGTCTCCAGATTTCCAACCTAATATAGCGAAGAAATATATTGATCAGAAGTTTG TATTGCAGCTTTTAGAGCTCTTTGACAGTGAAGATCCTCGGGAGAGAGATTTTCTTAAAACCACCCTTCA CAGAATCTATGGGAAATTCCTAGGCTTGAGAGCTTACATCAGAAAACAGATAAATAATATATTTTATAGG TTTATTTATGAAACAGAGCATCATAATGGCATAGCAGAGTTACTGGAAATATTGGGAAGTATAATTAATG GATTTGCCTTACCACTAAAAGAAGAGCACAAGATTTTCTTATTGAAGGTGTTACTACCTTTGCACAAAGT GAAATCTCTGAGTGTCTACCATCCCCAGCTGGCATACTGTGTAGTGCAGTTTTTAGAAAAGGACAGCACC CTCACGGAACCAGTGGTGATGGCACTTCTCAAATACTGGCCAAAGACTCACAGTCCAAAAGAAGTAATGT TCTTAAACGAATTAGAAGAGATTTTAGATGTCATTGAACCATCAGAATTTGTGAAGATCATGGAACCCCT CTTCCGGCAGTTGGCCAAATGTGTCTCCAGCCCACACTTCCAGGTGGCAGAGCGAGCTCTCTATTACTGG AATAATGAATACATCATGAGTTTAATCAGTGACAACGCAGCGAAGATTCTGCCCATCATGTTTCCTTCCT TGTACCGCAACTCAAAGACCCATTGGAACAAGACAATACATGGCTTGATATACAACGCCCTGAAGCTCTT CATGGAGATGAACCAAAAGCTATTTGATGACTGTACACAACAGTTCAAAGCAGAGAAACTAAAAGAGAAG CTAAAAATGAAAGAACGGGAAGAAGCATGGGTTAAAATAGAAAATCTAGCCAAAGCCAATCCCCAGTACA CAGTGTATAGTCAAGCCAGCACCATGAGCATTCCGGTTGCAATGGAGACAGATGGGCCTTTATTTGAAGA TGTGCAGATGCTGAGAAAGACAGTGAAGGACGAGGCTCATCAGGCACAGAAAGATCCGAAGAAGGACCGT CCTCTTGCACGCCGCAAGTCCGAGCTGCCTCAGGACCCCCACACCAAGAAAGCCTTGGAAGCTCACTGCA GGGCCGATGAGCTGGCCTCCCAGGACGGCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214000 representing NM_002719
Red=Cloning site Green=Tags(s) MLTCNKAGSRMVVDAANSNGPFQPVVLLHIRDVPPADQEKLFIQKLRQCCVLFDFVSDPLSDLKWKEVKR AALSEMVEYITHNRNVITEPIYPEVVHMFAVNMFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEF FLRFLESPDFQPNIAKKYIDQKFVLQLLELFDSEDPRERDFLKTTLHRIYGKFLGLRAYIRKQINNIFYR FIYETEHHNGIAELLEILGSIINGFALPLKEEHKIFLLKVLLPLHKVKSLSVYHPQLAYCVVQFLEKDST LTEPVVMALLKYWPKTHSPKEVMFLNELEEILDVIEPSEFVKIMEPLFRQLAKCVSSPHFQVAERALYYW NNEYIMSLISDNAAKILPIMFPSLYRNSKTHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQFKAEKLKEK LKMKEREEAWVKIENLAKANPQYTVYSQASTMSIPVAMETDGPLFEDVQMLRKTVKDEAHQAQKDPKKDR PLARRKSELPQDPHTKKALEAHCRADELASQDGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002719 |
ORF Size | 1572 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002719.2, NM_002719.3, NP_002710.2 |
RefSeq Size | 4167 bp |
RefSeq ORF | 1575 bp |
Locus ID | 5527 |
Cytogenetics | 14q32.31 |
Domains | B56 |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | Oocyte meiosis, Wnt signaling pathway |
MW | 60.9 kDa |
Gene Summary | 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a gamma isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109608 | PPP2R5C (untagged)-Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1 |
USD 890.00 |
|
RG214000 | PPP2R5C (GFP-tagged) - Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1 |
USD 520.00 |
|
RC214000L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1, Myc-DDK-tagged |
USD 828.00 |
|
RC214000L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1, mGFP tagged |
USD 670.00 |
|
RC214000L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1, Myc-DDK-tagged |
USD 670.00 |
|
RC214000L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B', gamma (PPP2R5C), transcript variant 1, mGFP tagged |
USD 670.00 |
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