PDE4C (NM_001098818) Human Tagged ORF Clone

CAT#: RC214274

PDE4C (Myc-DDK-tagged)-Human phosphodiesterase 4C, cAMP-specific (PDE4C), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY420350) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214274 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PDE4C
• Synonyms: DPDE1; PDE21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214274 representing NM_001098818
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGGGCCCCCCCGCGCCCGCCCCGGTCCCCGGGCCCGGCTCCCCTCGGGGATCCCCGCGCGGCTCCC
  CCGGGCTCTTCAGGAAGCTTTTGGTGAATCAGAGCATCCGCCTGCAGCGGCGCTTCACGGTGGCCCATCC
  GCTGTGCTTTGACCTGGAAAATGGGCTCTCGTGTGGGAGGAGGGCCCTGGACCCTCAGTCCAGCCCTGGC
  CTGGGCCGGATTATGCAGGCTCCAGTCCCGCACAGCCAGCGGCGCGAGTCCTTCCTGTACCGCTCAGATA
  GCGACTATGAACTCTCGCCCAAGGCCATGTCTCGGAACTCCTCTGTGGCCAGCGACCTACATGGAGAGGA
  CATGATTGTGACGCCCTTTGCCCAGGTCCTGGCCAGTCTGCGGACCGTTCGGAGCAACGTGGCGGCCCTT
  GCCCGCCAGCAATGCCTAGGAGCAGCCAAGCAGGGACCCGTCGGAAACCCTTCATCCAGCAATCAGCTCC
  CTCCTGCAGAGGACACGGGGCAGAAGCTGGCATTGGAGACGCTAGACGAGCTGGACTGGTGCCTGGATCA
  GTTGGAGACGCTGCAGACCCGGCACTCGGTGGGGGAGATGGCCTCCAACAAGTTCAAGCGGATCCTGAAC
  CGGGAGTTGACCCACCTGTCCGAAACCAGCCGCTCCGGGAACCAGGTGTCCGAGTACATCTCCCGGACCT
  TCCTGGACCAGCAGACCGAGGTGGAGCTGCCCAAGGTGACCGCTGAGGAGGCCCCACAGCCCATGTCCCG
  GATCAGTGGCCTACATGGGCTCTGCCACAGTGCCAGCCTCTCCTCAGCCACTGTCCCACGCTTTGGGGTC
  CAGACTGACCAGGAGGAGCAACTGGCCAAGGAGCTAGAAGACACCAACAAGTGGGGACTTGATGTGTTCA
  AGGTGGCGGAGCTAAGTGGGAACCGGCCCCTCACAGCTATCATATTCAGCATTTTTCAGGAGCGGGACCT
  GCTGAAGACATTCCAGATCCCAGCAGACACACTGGCCACCTACCTGCTGATGCTGGAAGGTCACTACCAC
  GCCAATGTGGCCTACCACAACAGCCTACATGCCGCCGACGTGGCCCAGTCCACGCATGTGCTGCTGGCTA
  CGCCCGCCCTCGAGGCTGTGTTCACAGACTTGGAAATCCTGGCTGCCCTCTTTGCAAGCGCCATCCACGA
  CGTGGACCATCCTGGGGTCTCCAACCAGTTTCTGATTAACACCAACTCAGAGCTGGCGCTTATGTACAAC
  GACGCCTCGGTGCTGGAGAACCATCACCTGGCTGTGGGCTTCAAGCTGCTGCAGGCAGAGAACTGCGATA
  TCTTCCAGAACCTCAGCGCCAAGCAGCGACTGAGTCTGCGCAGGATGGTCATTGACATGGTGCTGGCCAC
  AGACATGTCCAAACACATGAACCTCCTGGCCGACCTCAAGACCATGGTGGAGACCAAGAAGGTGACAAGC
  CTCGGTGTCCTCCTCCTGGACAACTATTCCGACCGAATCCAGGTCTTGCAGAACCTGGTGCACTGTGCTG
  ATCTGAGCAACCCCACCAAGCCGCTGCCCCTGTACCGCCAGTGGACGGACCGCATCATGGCCGAGTTCTT
  CCAGCAGGGAGACCGCGAGCGTGAGTCGGGCCTGGACATCAGTCCCATGTGTGACAAGCATACGGCCTCA
  GTGGAGAAGTCCCAGGTGGGTTTCATTGACTACATTGCTCACCCACTGTGGGAGACTTGGGCTGACCTGG
  TCCACCCAGATGCACAGGACCTGCTGGACACGCTGGAGGACAATCGAGAGTGGTACCAGAGCAAGATCCC
  CCGAAGTCCCTCAGACCTCACCAACCCCGAGCGGGACGGGCCTGACAGATTCCAGTTTGAACTGACTCTG
  GAGGAGGCAGAGGAAGAGGATGAGGAGGAAGAAGAGGAGGGGGAAGAGACAGCTTTAGCCAAAGAGGCCT
  TGGAGTTGCCTGACACTGAACTCCTGTCCCCTGAAGCCGGCCCAGACCCTGGGGACTTACCCCTCGACAA
  CCAGAGGACT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214274 representing NM_001098818
  Red=Cloning site Green=Tags(s)
  
  MQGPPAPAPVPGPGSPRGSPRGSPGLFRKLLVNQSIRLQRRFTVAHPLCFDLENGLSCGRRALDPQSSPG
  LGRIMQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAAL
  ARQQCLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILN
  RELTHLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGV
  QTDQEEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYH
  ANVAYHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYN
  DASVLENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTS
  LGVLLLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTAS
  VEKSQVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTL
  EEAEEEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001098818
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001098818.1, NM_001098818.2, NM_001098818.3, NP_001092288.1
• RefSeq Size: 5034 bp
• RefSeq ORF: 2043 bp
• Locus ID: 5143
• Cytogenetics: 19p13.11
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• MW: 75.4 kDa
• Gene Summary: 'The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]'