SLC36A1 (NM_078483) Human Tagged ORF Clone

CAT#: RC214295

SLC36A1 (Myc-DDK-tagged)-Human solute carrier family 36 (proton/amino acid symporter), member 1 (SLC36A1)

Product Images

Western blot validation of overexpression lysate (Cat# LY409191) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214295 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC36A1
• Synonyms: Dct1; LYAAT1; PAT1; TRAMD3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214295 representing NM_078483
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCACGCAGAGACTTCGGAATGAAGACTACCACGACTACAGCTCCACGGACGTGAGCCCTGAGGAGA
  GCCCGTCGGAAGGCCTCAACAACCTCTCCTCCCCGGGCTCCTACCAGCGCTTTGGTCAAAGCAATAGCAC
  AACATGGTTCCAGACCTTGATCCACCTGTTAAAAGGCAACATTGGCACAGGACTCCTGGGACTCCCTCTG
  GCGGTGAAAAATGCAGGCATCGTGATGGGTCCCATCAGCCTGCTGATCATAGGCATCGTGGCCGTGCACT
  GCATGGGTATCCTGGTGAAATGTGCTCACCACTTCTGCCGCAGGCTGAATAAATCCTTTGTGGATTATGG
  TGATACTGTGATGTATGGACTAGAATCCAGCCCCTGCTCCTGGCTCCGGAACCACGCACACTGGGGAAGA
  CGTGTTGTGGACTTCTTCCTGATTGTCACCCAGCTGGGATTCTGCTGTGTCTATTTTGTGTTTCTGGCTG
  ACAACTTTAAACAGGTGATAGAAGCGGCCAATGGGACCACCAATAACTGCCACAACAATGAGACGGTGAT
  TCTGACGCCTACCATGGACTCGCGACTCTACATGCTCTCCTTCCTGCCCTTCCTGGTGCTGCTGGTTTTC
  ATCAGGAACCTCCGAGCCCTGTCCATCTTCTCCCTGTTGGCCAACATCACCATGCTGGTCAGCTTGGTCA
  TGATCTACCAGTTCATTGTTCAGAGGATCCCAGACCCCAGCCACCTCCCCTTGGTGGCCCCTTGGAAGAC
  CTACCCTCTCTTCTTTGGCACAGCGATTTTTTCATTTGAAGGCATTGGAATGGTTCTGCCCCTGGAAAAC
  AAAATGAAGGATCCTCGGAAGTTCCCACTCATCCTGTACCTGGGCATGGTCATCGTCACCATCCTCTACA
  TCAGCCTGGGGTGTCTGGGGTACCTGCAATTTGGAGCTAATATCCAAGGCAGCATAACCCTCAACCTGCC
  CAACTGCTGGTTGTACCAGTCAGTTAAGCTGCTGTACTCCATCGGGATCTTTTTCACCTACGCACTCCAG
  TTCTACGTCCCGGCTGAGATCATCATCCCCTTCTTTGTGTCCCGAGCGCCCGAGCACTGTGAGTTAGTGG
  TGGACCTGTTTGTGCGCACAGTGCTGGTCTGCCTGACATGCATCTTGGCCATCCTCATCCCCCGCCTGGA
  CCTGGTCATCTCCCTGGTGGGCTCCGTGAGCAGCAGCGCCCTGGCCCTCATCATCCCACCGCTCCTGGAG
  GTCACCACCTTCTACTCAGAGGGCATGAGCCCCCTCACCATCTTTAAGGACGCCCTGATCAGCATCCTGG
  GCTTCGTGGGCTTTGTGGTGGGGACCTATGAGGCTCTCTATGAGCTGATCCAGCCAAGCAATGCTCCCAT
  CTTCATCAATTCCACCTGTGCCTTCATA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214295 representing NM_078483
  Red=Cloning site Green=Tags(s)
  
  MSTQRLRNEDYHDYSSTDVSPEESPSEGLNNLSSPGSYQRFGQSNSTTWFQTLIHLLKGNIGTGLLGLPL
  AVKNAGIVMGPISLLIIGIVAVHCMGILVKCAHHFCRRLNKSFVDYGDTVMYGLESSPCSWLRNHAHWGR
  RVVDFFLIVTQLGFCCVYFVFLADNFKQVIEAANGTTNNCHNNETVILTPTMDSRLYMLSFLPFLVLLVF
  IRNLRALSIFSLLANITMLVSLVMIYQFIVQRIPDPSHLPLVAPWKTYPLFFGTAIFSFEGIGMVLPLEN
  KMKDPRKFPLILYLGMVIVTILYISLGCLGYLQFGANIQGSITLNLPNCWLYQSVKLLYSIGIFFTYALQ
  FYVPAEIIIPFFVSRAPEHCELVVDLFVRTVLVCLTCILAILIPRLDLVISLVGSVSSSALALIIPPLLE
  VTTFYSEGMSPLTIFKDALISILGFVGFVVGTYEALYELIQPSNAPIFINSTCAFI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_078483
• ORF Size: 1428 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_078483.2, NM_078483.3, NP_510968.2
• RefSeq Size: 5821 bp
• RefSeq ORF: 1431 bp
• Locus ID: 206358
• Domains: Aa_trans
• Protein Families: Transmembrane
• MW: 52.9 kDa
• Gene Summary: This gene encodes a member of the eukaryote-specific amino acid/auxin permease (AAAP) 1 transporter family. The encoded protein functions as a proton-dependent, small amino acid transporter. This gene is clustered with related family members on chromosome 5q33.1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]