OR2K2 (NM_205859) Human Tagged ORF Clone

CAT#: RC214362

OR2K2 (Myc-DDK-tagged)-Human olfactory receptor, family 2, subfamily K, member 2 (OR2K2)

Product Images

Western blot validation of overexpression lysate (Cat# LY404207) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214362 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR2K2
• Synonyms: HSHTPCRH06; HTPCRH06; OR2AN1P; OR2AR1P
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214362 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAGGAGAAAACTTCACCATTTGGAGCATTTTTTTCTTGGAGGGATTTTCCCAGTACCCAGGGTTAG
  AAGTGGTTCTCTTCGTCTTCAGCCTTGTAATGTATCTGACAACGCTCTTGGGCAACAGCACTCTTATTTT
  GATCACTATCCTAGATTCACGCCTTAAAACCCCCATGTACTTATTCCTTGGAAATCTCTCTTTCATGGAT
  ATTTGTTACACATCTGCCTCTGTTCCTACTTTGCTGGTGAACTTGCTGTCATCCCAGAAAACCATTATCT
  TTTCTGGGTGTGCTGTACAGATGTATCTGTCCCTTGCCATGGGCTCCACAGAGTGTGTGCTCCTGGCCGT
  GATGGCATATGACCGTTATGTGGCCATTTGTAACCCGCTGAGATACTCCATCATCATGAACAGGTGCGTC
  TGTGCACGGATGGCTACGGTCTCCTGGGTGACGGGTTGCCTGACCGCTCTGCTGGAAACCAGTTTTGCCC
  TGCAGATACCCCTCTGTGGGAATCTCATCGATCACTTCACGTGTGAAATTCTGGCGGTGCTAAAGTTAGC
  TTGCACAAGTTCACTGCTCATGAACACCATCATGCTGGTGGTCAGCATTCTCCTCTTGCCAATTCCAATG
  CTCTTAGTTTGCATCTCTTACATCTTCATCCTTTCCACTATTCTGAGAATCACCTCAGCAGAGGGAAGAA
  ACAAGGCTTTTTCTACCTGTGGTGCCCATTTGACTGTGGTGATTTTGTATTATGGGGCTGCCCTCTCTAT
  GTACCTAAAGCCTTCTTCATCAAATGCACAAAAAATAGACAAAATCATCTCGTTGCTTTACGGAGTGCTT
  ACCCCTATGTTGAACCCCATAATTTACAGTTTAAGAAACAAGGAAGTCAAAGATGCTATGAAGAAATTGC
  TGGGCAAAATAACATTGCATCAAACACACGAACATCTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214362 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQGENFTIWSIFFLEGFSQYPGLEVVLFVFSLVMYLTTLLGNSTLILITILDSRLKTPMYLFLGNLSFMD
  ICYTSASVPTLLVNLLSSQKTIIFSGCAVQMYLSLAMGSTECVLLAVMAYDRYVAICNPLRYSIIMNRCV
  CARMATVSWVTGCLTALLETSFALQIPLCGNLIDHFTCEILAVLKLACTSSLLMNTIMLVVSILLLPIPM
  LLVCISYIFILSTILRITSAEGRNKAFSTCGAHLTVVILYYGAALSMYLKPSSSNAQKIDKIISLLYGVL
  TPMLNPIIYSLRNKEVKDAMKKLLGKITLHQTHEHL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_205859
• ORF Size: 950 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_205859.1, NP_995581.1
• RefSeq Size: 951 bp
• RefSeq ORF: 951 bp
• Locus ID: 26248
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 35 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]