Rho GTPase activating protein 24 (ARHGAP24) (NM_031305) Human Tagged ORF Clone

CAT#: RC214411

ARHGAP24 (Myc-DDK-tagged)-Human Rho GTPase activating protein 24 (ARHGAP24), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY410555) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214411 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGAP24
• Synonyms: FILGAP; p73; p73RhoGAP; RC-GAP72; RCGAP72
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214411 representing NM_031305
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTGAAGACCGGAATTCTGGGGGGTGCCCGGCTGGTGCCTTAGCCTCAACTCCTTTCATCCCTAAAA
  CTACATACAGAAGAATCAAACGGTGTTTTAGTTTTCGGAAAGGCATTTTTGGACAGAAACTGGAGGATAC
  TGTTCGTTATGAGAAGAGATATGGGAACCGTCTGGCTCCGATGTTGGTGGAGCAGTGCGTGGACTTTATC
  CGACAAAGGGGGCTGAAAGAAGAGGGTCTCTTTCGACTGCCAGGCCAGGCTAATCTTGTTAAGGAGCTCC
  AAGATGCCTTTGACTGTGGGGAGAAGCCATCATTTGACAGCAACACAGATGTACACACGGTGGCATCACT
  TCTTAAGCTGTACCTCCGAGAACTTCCAGAACCAGTTATTCCTTATGCGAAGTATGAAGATTTTTTGTCA
  TGTGCCAAACTGCTCAGCAAGGAAGAGGAAGCAGGTGTTAAGGAATTAGCAAAGCAGGTGAAGAGTTTGC
  CAGTGGTAAATTACAACCTCCTCAAGTATATTTGCAGATTCTTGGATGAAGTACAGTCCTACTCGGGAGT
  TAACAAAATGAGTGTGCAGAACTTGGCAACGGTCTTTGGTCCTAATATCCTGCGCCCCAAAGTGGAAGAT
  CCTTTGACTATCATGGAGGGCACTGTGGTGGTCCAGCAGTTGATGTCAGTGATGATTAGCAAACATGATT
  GCCTCTTTCCCAAAGATGCAGAACTACAAAGCAAGCCCCAAGATGGAGTGAGCAACAACAATGAAATTCA
  GAAGAAAGCCACCATGGGGCAGTTACAGAACAAGGAGAACAATAACACCAAGGACAGCCCTAGTAGGCAG
  TGCTCCTGGGACAAGTCTGAGTCACCCCAGAGAAGCAGCATGAACAATGGATCCCCCACAGCTCTATCAG
  GCAGCAAAACCAACAGCCCAAAGAACAGTGTTCACAAGCTAGATGTGTCTAGAAGCCCCCCTCTCATGGT
  CAAAAAGAACCCAGCCTTTAATAAGGGTAGTGGGATAGTTACCAATGGGTCCTTCAGCAGCAGTAATGCA
  GAAGGTCTTGAGAAAACCCAAACCACCCCCAATGGGAGCCTACAGGCCAGAAGGAGCTCTTCACTGAAGG
  TATCTGGTACCAAAATGGGCACGCACAGTGTACAGAATGGAACGGTGCGCATGGGCATTTTGAACAGCGA
  CACACTCGGGAACCCCACAAATGTTCGAAACATGAGCTGGCTGCCAAATGGCTATGTGACCCTGAGGGAT
  AACAAGCAGAAAGAACAAGCTGGAGAGTTAGGCCAGCACAACAGACTGTCCACCTATGATAATGTCCATC
  AACAGTTCTCCATGATGAACCTTGATGACAAGCAGAGCATTGACAGTGCTACCTGGTCCACTTCCTCCTG
  TGAAATCTCCCTCCCTGAGAACTCCAACTCCTGTCGCTCTTCTACCACCACCTGCCCAGAGCAAGACTTT
  TTTGGGGGGAACTTTGAGGACCCTGTTTTGGATGGGCCCCCGCAGGACGACCTTTCCCACCCCAGGGACT
  ATGAAAGCAAAAGTGACCACAGGAGTGTGGGAGGTCGAAGTAGTCGTGCCACCAGTAGCAGTGACAACAG
  TGAGACATTTGTGGGCAACAGCAGCAGCAACCACAGTGCACTGCACAGTTTAGTTTCCAGCCTGAAACAG
  GAAATGACCAAACAGAAGATAGAGTATGAGTCCAGGATAAAGAGCTTAGAACAGCGAAACTTGACTTTGG
  AAACAGAAATGATGAGCCTCCATGATGAACTGGATCAGGAGAGGAAAAAGTTCACAATGATAGAAATAAA
  AATGCGAAATGCCGAGCGAGCAAAAGAAGATGCCGAGAAAAGAAATGACATGCTACAGAAAGAAATGGAG
  CAGTTTTTTTCCACGTTTGGAGAACTGACAGTGGAACCCAGGAGAACCGAGAGAGGAAACACAATATGGA
  TTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214411 representing NM_031305
  Red=Cloning site Green=Tags(s)
  
  MPEDRNSGGCPAGALASTPFIPKTTYRRIKRCFSFRKGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFI
  RQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLS
  CAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVED
  PLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNNTKDSPSRQ
  CSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNA
  EGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRD
  NKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSSTTTCPEQDF
  FGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALHSLVSSLKQ
  EMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEME
  QFFSTFGELTVEPRRTERGNTIWIQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031305
• ORF Size: 1965 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_031305.1, NM_031305.2, NP_112595.1, NP_112595.2
• RefSeq Size: 4593
• RefSeq ORF: 1968
• Locus ID: 83478
• Domains: RhoGAP
• MW: 73.2 kDa
• Gene Summary: This gene encodes a Rho-GTPase activating protein, which is specific for the small GTPase family member Rac. Binding of the encoded protein by filamin A targets it to sites of membrane protrusion, where it antognizes Rac. This results in suppression of lamellae formation and promotion of retraction to regulate cell polarity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2016]