KY (NM_178554) Human Tagged ORF Clone

CAT#: RC214443

KY (Myc-DDK-tagged)-Human kyphoscoliosis peptidase (KY)

Product Images

Western blot validation of overexpression lysate (Cat# LY405923) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214443 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KY
• Synonyms: MFM7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214443 representing NM_178554
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGAAGAAGGACATCAACGCTGTATCTATCGACATGCTGCTGATCGTGCACTCGGAGAAGCGGC
  GCGCCGCACAGGGTACGCTCTCAGACCAGCAGGCGAACCCGAGCTCGCTGCTGCAGCGCGGAGGAGGATT
  CCAAGGTGTTGGAAATGGAGTCCGAAGATGGCAGAAATTAGAAGGAAATGACTTTCACGAAAACTTGGTG
  GAGAAGCAGCACCCTCAGCAGCCCCAGGTCATCACTTCCTACAACAGCCAAGGGACACAACTGACTGTGG
  AAGTCCACCCTCGAGATGCCATGCCCCAGCTGCTCAAGAAGTTCTCCTTGGCTAAACGTTTACAAGGTGA
  TAAAAATGGAAACACAAGACCCCGGCAACCTGGAGGGAAAGATGCCCATGCCTACCCCTGGGATCGATCT
  AGCCTGAAATCCATGTCCCTGGACCTGCAGCAGTTTGAGAAATTGGATATCTACGCCTCACAGGTGACAG
  CCAAGAGTGGCCTAGACGAACTGGTGAGTGACCTGCTCCAGGAGGCCCACACTGACCTGGAAAGGGTCCG
  CGCCATCTGGATCTGGATCTGCCATCACATAGAGTATGACATTGCAGCTGCTCAGGAGAAGGACCGCCAA
  GCCTTCAAACCCACTGACATCCTGCGGACCCAGAAGACCAACTGTGATGGCTATGCTGGCCTCTTCGAGA
  GAATGTGCAGGCTCGCCGGAGTGCAGTGTATGACCGTGCCTGGCTACTCCAAGGGTTTCGGCTACCAGAC
  AGGGCAGAGCTTCTCGGGGGAGTTTGACCATGCCTGGAATGCTGTGTACCTGGAGGGAAGATGGCACCTG
  GTGGACAGCACCTGGGGCAGCGGCCTGGTGGACACCATCACCTCCAAATTCACCTTCCTCTACAATGAGT
  TCTACTTCCTCACCCACCCTGCACTGTTCATCGAGGACCACTTCCCAGACAACAAGAACTGGCAGCTGCT
  AAAACCTCCTCAATCTCTGAGGCAGTTTGAGAACAACATGTATCACAAGAGTGAATTCTACAACAAAGGG
  ATGCTGAGTGCCCACCCAGAGACTTCCATGATCAGAACAGTGAATGGGAAGGCCACGGTCACCATTGAGA
  GCTGCGCCCCGACGCTGTTCATGTTCATGCTCAATGGCAAGCAAGAGCATGGGCTGCTGAGCCTAAGGAA
  GAATGGGATGAAGTTGGAGGTGTACCCTCCAACCATGGGCACTCACAAGCTGCAGATCTTTGCCAAGGGC
  AACTCCGACATCTACAGCTCAGTGCTGGAGTACACGCTCAAGTGCAATTATGTGGACATGGGTGTCCAGC
  TGCCTGCTGAGCTTCACCAGCCCGTGGGCCCCAGCTGGTTCTCGGAGCAGATGGGCATCATGAAGCCCTC
  CCACCCTGACCCTATCATCCACACCAGCGACGGGCGCTGCTCCATCAGCTTCAGCGTGGAGGAGGGCATT
  AATGTCCTGGCTTCCCTCCACGGGGATGATGGCCCCATCACTGAGGAGACACAGCGGCGCTACATCTTCC
  AGCTGCACCGGGAGAAGCAGACCGAGCTGAAAGTCCAGCTGCCCCATGCAGGCAAGTTTGCCCTCAAGAT
  CTTTGTCAAGAAGAGGCAGGAACCAGGAAACTACATCTTCGTCTTTAATTACCTTGTATGCTGTGCCAAC
  ACCAAGGTGAACTGGCCCATGTTCCCTGAGAGCTTTGGCAACTGGGGACAGGACAATGAGCTGCTGGAAC
  CTCTGTCAGGTGTGCTTCCTGCCAACCGGAATGTCCCATTCAAATTGAAGCTGCATGGTATTGCCAAAGT
  CCTGGTGAAGGGGCAGGACACATGGCCCCTGACCCTGAACCACGAGGGCTACTGGGAGGGAAGCTGCAGC
  ACAGCTGGCTGCCAGGAAGTCTATGTCATGGTGCTGGAGAATGCCAACCACAATTTCTACTCCTACATCC
  TGAAATACAAAGTGAATGCCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214443 representing NM_178554
  Red=Cloning site Green=Tags(s)
  
  MELKKDINAVSIDMLLIVHSEKRRAAQGTLSDQQANPSSLLQRGGGFQGVGNGVRRWQKLEGNDFHENLV
  EKQHPQQPQVITSYNSQGTQLTVEVHPRDAMPQLLKKFSLAKRLQGDKNGNTRPRQPGGKDAHAYPWDRS
  SLKSMSLDLQQFEKLDIYASQVTAKSGLDELVSDLLQEAHTDLERVRAIWIWICHHIEYDIAAAQEKDRQ
  AFKPTDILRTQKTNCDGYAGLFERMCRLAGVQCMTVPGYSKGFGYQTGQSFSGEFDHAWNAVYLEGRWHL
  VDSTWGSGLVDTITSKFTFLYNEFYFLTHPALFIEDHFPDNKNWQLLKPPQSLRQFENNMYHKSEFYNKG
  MLSAHPETSMIRTVNGKATVTIESCAPTLFMFMLNGKQEHGLLSLRKNGMKLEVYPPTMGTHKLQIFAKG
  NSDIYSSVLEYTLKCNYVDMGVQLPAELHQPVGPSWFSEQMGIMKPSHPDPIIHTSDGRCSISFSVEEGI
  NVLASLHGDDGPITEETQRRYIFQLHREKQTELKVQLPHAGKFALKIFVKKRQEPGNYIFVFNYLVCCAN
  TKVNWPMFPESFGNWGQDNELLEPLSGVLPANRNVPFKLKLHGIAKVLVKGQDTWPLTLNHEGYWEGSCS
  TAGCQEVYVMVLENANHNFYSYILKYKVNAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178554
• ORF Size: 1983 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178554.4, NP_848649.3
• RefSeq Size: 5718
• RefSeq ORF: 1986
• Locus ID: 339855
• MW: 75 kDa
• Gene Summary: The protein encoded by this gene belongs to the transglutaminase-like superfamily. The protein is involved in the function, maturation and stabilization of the neuromuscular junction and may be required for normal muscle growth. Mutations in this gene are associated with myopathy, myofibrillar, 7. [provided by RefSeq, Apr 2017]