GRID2 (NM_001510) Human Tagged ORF Clone
CAT#: RC214449
GRID2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2)
"NM_001510" in other vectors (4)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GRID2 |
Synonyms | GluD2; SCAR18 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214449 representing NM_001510
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTTTTCCCCTTTCTCTTGGTTTTGTCCGTCTGGTGGTCTCGAACCTGGGACTCGGCGAATGCGG ATTCGATCATTCACATCGGAGCAATTTTTGATGAATCTGCCAAAAAGGATGATGAGGTATTTCGCACTGC GGTTGGTGACCTTAACCAGAATGAGGAGATCTTACAGACTGAGAAAATCACATTTTCAGTGACGTTTGTT GATGGCAACAACCCTTTCCAAGCAGTTCAAGAAGCCTGTGAACTTATGAATCAAGGCATCTTGGCCCTGG TCAGCTCCATTGGCTGCACGTCAGCAGGATCCCTCCAGTCTTTGGCAGACGCCATGCATATCCCCCACCT CTTCATTCAGCGCTCAACAGCTGGGACCCCAAGGAGTGGCTGTGGACTCACCCGGAGCAACAGGAATGAT GACTACACTCTCTCAGTTCGCCCACCTGTCTACTTGCATGATGTTATCCTAAGAGTGGTCACAGAGTATG CCTGGCAGAAATTCATTATATTCTATGATAGTGAATACGATATCCGTGGAATACAGGAGTTCTTGGACAA AGTCTCTCAGCAGGGAATGGATGTTGCACTTCAGAAGGTAGAAAACAACATCAATAAAATGATTACCACT CTCTTTGACACCATGAGAATAGAAGAACTGAATCGCTATCGAGACACTCTTAGGCGAGCGATCCTTGTTA TGAATCCTGCTACAGCCAAATCCTTCATTACTGAGGTTGTGGAGACTAATTTGGTTGCTTTTGACTGTCA CTGGATCATTATAAATGAGGAAATAAACGATGTGGACGTACAGGAACTTGTAAGAAGGTCAATTGGAAGG TTAACGATTATTCGGCAGACATTTCCAGTTCCCCAGAACATAAGTCAGCGGTGTTTCCGTGGCAACCATC GAATATCTTCAACATTGTGTGATCCAAAGGATCCATTTGCTCAGAATATGGAGATTTCCAACCTTTACAT ATATGACACGGTGCTTCTGCTTGCTAATGCTTTTCATAAGAAGCTGGAGGACCGAAAGTGGCACAGCATG GCAAGTCTGTCATGTATCAGAAAGAACTCAAAGCCCTGGCAGGGTGGGCGCTCCATGTTGGAGACCATCA AGAAGGGTGGAGTTAGTGGGTTGACTGGAGAGCTAGAATTTGGAGAAAATGGAGGCAATCCCAATGTCCA CTTTGAAATCCTTGGAACCAACTATGGAGAAGAGCTTGGCAGAGGTGTTCGAAAACTTGGTTGCTGGAAT CCTGTCACAGGTCTGAATGGGTCACTGACTGACAAGAAATTGGAGAATAACATGCGTGGAGTGGTTCTAC GTGTAGTAACTGTTCTGGAAGAACCTTTTGTGATGGTCTCTGAAAATGTCTTGGGTAAGCCGAAGAAATA CCAGGGCTTCTCCATTGATGTTTTGGATGCCTTATCTAACTACCTGGGTTTTAACTACGAAATTTACGTA GCACCGGATCACAAATACGGAAGCCCACAAGAAGATGGGACATGGAATGGCTTGGTAGGAGAACTTGTCT TTAAGAGAGCCGACATAGGGATTTCTGCTTTAACCATCACTCCAGATCGTGAAAATGTGGTGGACTTTAC GACACGTTACATGGACTACTCAGTGGGGGTACTACTTCGAAGGGCTGAAAAGACAGTGGATATGTTTGCC TGTCTTGCACCATTTGATCTCTCTCTATGGGCTTGCATTGCTGGCACAGTCCTTCTGGTGGGTCTACTGG TCTACCTCTTGAACTGGCTTAATCCCCCACGATTACAAATGGGATCAATGACGTCTACTACTCTCTACAA CTCCATGTGGTTTGTGTATGGATCTTTTGTACAACAAGGCGGGGAAGTCCCGTACACGACTCTGGCTACC CGAATGATGATGGGGGCTTGGTGGCTATTTGCTTTGATTGTTATCTCATCTTACACGGCAAACCTCGCTG CTTTCCTCACTATTACACGCATTGAAAGTTCCATCCAGTCTCTCCAGGACCTTTCCAAGCAAACAGAAAT CCCTTATGGCACAGTCCTAGACTCTGCGGTATATGAGCATGTCCGCATGAAAGGACTGAATCCTTTTGAG AGGGACAGCATGTATTCCCAAATGTGGCGGATGATCAACCGAAGCAATGGATCGGAGAACAATGTTCTGG AGTCCCAGGCAGGCATTCAAAAGGTAAAATATGGAAATTATGCTTTCGTATGGGATGCAGCTGTATTGGA ATATGTGGCTATCAATGACCCAGATTGTTCCTTTTACACCATTGGAAATACTGTTGCTGATCGGGGATAT GGAATTGCATTACAACATGGCAGTCCTTACCGAGATGTTTTTTCACAAAGGATCCTGGAGCTTCAGCAGA ATGGTGACATGGACATCCTGAAGCACAAATGGTGGCCTAAGAATGGCCAGTGTGACCTGTACTCGTCAGT GGACACAAAGCAGAAAGGAGGCGCCCTGGACATAAAGAGCTTTGCAGGGGTCTTTTGTATCCTGGCTGCT GGAATTGTCCTCTCCTGCTTCATAGCCATGCTGGAGACGTGGTGGAACAAGAGGAAAGGCTCCCGGGTTC CATCAAAAGAGGATGACAAGGAAATTGACCTGGAGCACCTCCATAGACGTGTAAATAGCTTGTGCACAGA TGACGACAGCCCCCATAAACAGTTTTCCACCTCGTCAATTGATTTGACCCCTCTGGACATTGACACTTTG CCAACACGACAAGCACTGGAGCAAATCAGTGATTTCAGGAACACTCATATTACCACAACAACCTTTATCC CAGAGCAGATCCAGACTCTTAGCCGCACACTGTCAGCTAAAGCTGCTTCTGGTTTCACTTTTGGCAACGT GCCTGAGCACCGAACTGGCCCTTTTAGGCACAGGGCACCTAATGGGGGCTTTTTCAGGAGTCCTATAAAA ACAATGTCATCTATTCCTTATCAACCAACTCCTACCCTGGGGCTCAATCTGGGTAATGATCCAGACCGAG GCACCTCCATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214449 representing NM_001510
Red=Cloning site Green=Tags(s) MEVFPFLLVLSVWWSRTWDSANADSIIHIGAIFDESAKKDDEVFRTAVGDLNQNEEILQTEKITFSVTFV DGNNPFQAVQEACELMNQGILALVSSIGCTSAGSLQSLADAMHIPHLFIQRSTAGTPRSGCGLTRSNRND DYTLSVRPPVYLHDVILRVVTEYAWQKFIIFYDSEYDIRGIQEFLDKVSQQGMDVALQKVENNINKMITT LFDTMRIEELNRYRDTLRRAILVMNPATAKSFITEVVETNLVAFDCHWIIINEEINDVDVQELVRRSIGR LTIIRQTFPVPQNISQRCFRGNHRISSTLCDPKDPFAQNMEISNLYIYDTVLLLANAFHKKLEDRKWHSM ASLSCIRKNSKPWQGGRSMLETIKKGGVSGLTGELEFGENGGNPNVHFEILGTNYGEELGRGVRKLGCWN PVTGLNGSLTDKKLENNMRGVVLRVVTVLEEPFVMVSENVLGKPKKYQGFSIDVLDALSNYLGFNYEIYV APDHKYGSPQEDGTWNGLVGELVFKRADIGISALTITPDRENVVDFTTRYMDYSVGVLLRRAEKTVDMFA CLAPFDLSLWACIAGTVLLVGLLVYLLNWLNPPRLQMGSMTSTTLYNSMWFVYGSFVQQGGEVPYTTLAT RMMMGAWWLFALIVISSYTANLAAFLTITRIESSIQSLQDLSKQTEIPYGTVLDSAVYEHVRMKGLNPFE RDSMYSQMWRMINRSNGSENNVLESQAGIQKVKYGNYAFVWDAAVLEYVAINDPDCSFYTIGNTVADRGY GIALQHGSPYRDVFSQRILELQQNGDMDILKHKWWPKNGQCDLYSSVDTKQKGGALDIKSFAGVFCILAA GIVLSCFIAMLETWWNKRKGSRVPSKEDDKEIDLEHLHRRVNSLCTDDDSPHKQFSTSSIDLTPLDIDTL PTRQALEQISDFRNTHITTTTFIPEQIQTLSRTLSAKAASGFTFGNVPEHRTGPFRHRAPNGGFFRSPIK TMSSIPYQPTPTLGLNLGNDPDRGTSI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001510 |
ORF Size | 3021 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001510.1, NM_001510.2, NM_001510.3, NP_001501.1, NP_001501.2 |
RefSeq Size | 3024 bp |
RefSeq ORF | 3024 bp |
Locus ID | 2895 |
Cytogenetics | 4q22.1-q22.2 |
Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
Protein Pathways | Long-term depression, Neuroactive ligand-receptor interaction |
MW | 113.2 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC123987 | GRID2 (untagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) |
USD 2,030.00 |
|
RG214449 | GRID2 (GFP-tagged) - Human glutamate receptor, ionotropic, delta 2 (GRID2) |
USD 970.00 |
|
RC214449L3 | Lenti-ORF clone of GRID2 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) |
USD 1,080.00 |
|
RC214449L4 | Lenti-ORF clone of GRID2 (mGFP-tagged)-Human glutamate receptor, ionotropic, delta 2 (GRID2) |
USD 1,080.00 |
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