KA1 (GRIK4) (NM_014619) Human Tagged ORF Clone

CAT#: RC214488

GRIK4 (Myc-DDK-tagged)-Human glutamate receptor, ionotropic, kainate 4 (GRIK4)

Product Images

Western blot validation of overexpression lysate (Cat# LY415171) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214488 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIK4
• Synonyms: EAA1; GluK4; GRIK; KA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214488 representing NM_014619
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCCCGCGTCTCGGCGCCTTTGGTGCTGCTTCCTGCGTGGCTCGTGATGGTCGCCTGCAGCCCGCACT
  CCTTGAGGATCGCTGCTATCTTGGACGACCCCATGGAGTGCAGCAGAGGGGAGCGGCTCTCCATCACCCT
  GGCCAAGAACCGCATCAACCGCGCTCCTGAGAGGCTGGGCAAGGCCAAGGTCGAAGTGGACATCTTTGAG
  CTTCTCAGAGACAGCGAGTACGAGACTGCAGAAACCATGTGTCAGATCCTCCCCAAGGGGGTGGTCGCTG
  TCCTCGGACCATCGTCCAGCCCAGCCTCCAGCTCCATCATCAGCAACATCTGTGGAGAGAAGGAGGTCCC
  TCACTTCAAAGTGGCCCCAGAGGAGTTCGTCAAGTTCCAGTTCCAGAGATTCACAACCCTGAACCTCCAC
  CCCAGCAACACTGACATCAGCGTGGCTGTAGCTGGGATCCTGAACTTCTTCAACTGCACCACCGCCTGCC
  TCATCTGTGCCAAAGCAGAATGCCTTTTAAACCTAGAGAAGCTGCTCCGGCAATTCCTTATCTCCAAGGA
  CACGCTGTCCGTCCGCATGCTGGATGACACCCGGGACCCCACCCCGCTCCTCAAGGAGATCCGGGACGAC
  AAGACCGCCACCATCATCATCCACGCCAACGCCTCCATGTCCCACACCATCCTCCTGAAGGCAGCCGAAC
  TTGGGATGGTGTCAGCCTATTACACATACATCTTCACTAATCTGGAGTTCTCACTCCAGAGAATGGACAG
  CCTTGTGGATGATCGTGTCAACATCCTGGGATTTTCCATTTTCAACCAATCCCATGCTTTCTTCCAAGAG
  TTTGCCCAGAGCCTCAACCAGTCCTGGCAGGAGAACTGTGACCATGTGCCCTTCACTGGGCCTGCGCTCT
  CCTCGGCCCTGCTGTTTGATGCTGTCTATGCTGTGGTGACTGCGGTGCAGGAACTGAACCGGAGCCAAGA
  GATCGGCGTGAAGCCCTTGTCCTGCGGCTCGGCCCAGATCTGGCAGCACGGCACCAGCCTCATGAACTAC
  CTGCGCATGGTAGAATTGGAAGGTCTTACCGGCCACATTGAATTCAACAGCAAAGGCCAGAGGTCCAACT
  ACGCTTTGAAAATCTTACAGTTCACAAGGAATGGTTTTCGGCAGATCGGCCAGTGGCACGTGGCAGAGGG
  CCTCAGCATGGACAGCCACCTCTATGCCTCCAACATCTCGGACACTCTCTTCAACACCACCCTGGTCGTC
  ACCACCATCCTGGAAAACCCATATTTAATGCTGAAGGGGAACCACCAGGAGATGGAAGGCAATGACCGCT
  ACGAGGGCTTCTGTGTGGACATGCTCAAGGAGCTGGCAGAGATCCTCCGATTCAACTACAAGATCCGCCT
  GGTTGGGGATGGCGTGTACGGCGTTCCCGAGGCCAACGGCACCTGGACGGGAATGGTCGGGGAGCTGATC
  GCTAGGAAAGCAGATCTGGCTGTGGCAGGCCTCACCATTACAGCTGAACGGGAGAAGGTGATTGATTTCT
  CTAAGCCATTCATGACTCTGGGAATTAGCATTCTTTACCGCGTTCATATGGGACGCAAACCCGGCTATTT
  CTCCTTCCTGGACCCATTTTCTCCGGGCGTCTGGCTCTTCATGCTTCTAGCCTATCTGGCCGTCAGCTGT
  GTCCTCTTCCTGGTGGCTCGGTTGACGCCCTACGAGTGGTACAGCCCACACCCATGTGCCCAGGGCCGGT
  GCAACCTCCTGGTGAACCAGTACTCCCTGGGCAACAGCCTCTGGTTTCCGGTCGGGGGGTTCATGCAGCA
  AGGCTCCACCATCGCCCCTCGCGCCTTATCCACCCGCTGTGTCAGTGGCGTCTGGTGGGCATTCACGCTG
  ATCATCATCTCATCCTACACGGCCAACCTGGCAGCCTTCCTGACCGTGCAGCGCATGGATGTGCCCATTG
  AGTCAGTGGATGACCTGGCTGACCAGACCGCCATTGAATATGGCACAATTCACGGAGGCTCCAGCATGAC
  CTTCTTCCAAAATTCCCGCTACCAGACCTACCAACGCATGTGGAATTACATGTATTCCAAGCAGCCCAGC
  GTGTTCGTGAAGAGCACAGAGGAGGGAATCGCCAGGGTGTTGAATTCCAACTACGCCTTCCTCCTGGAAT
  CCACCATGAACGAGTACTATCGGCAGCGAAACTGCAACCTCACTCAGATTGGGGGCCTGCTGGACACCAA
  GGGCTATGGGATTGGCATGCCAGTCGGCTCGGTTTTCCGGGACGAGTTTGATCTGGCCATTCTCCAGCTG
  CAGGAGAACAACCGCCTGGAGATCCTGAAGCGCAAATGGTGGGAAGGAGGGAAGTGCCCCAAGGAGGAAG
  ATCACAGAGCTAAAGGCCTGGGAATGGAGAATATTGGTGGAATCTTTGTGGTTCTTATTTGTGGCTTAAT
  CGTGGCCATTTTTATGGCTATGTTGGAGTTTTTATGGACTCTCAGACACTCAGAAGCAACTGAGGTGTCC
  GTCTGCCAGGAGATGGTGACCGAGCTGCGCAGCATTATCCTGTGTCAGGACAGTATCCACCCCCGCCGGC
  GGCGCGCCGCAGTCCCGCCGCCCCGGCGCCCCATCCCCGAGGAGCGCCGACCGCGGGGCACGGCGACGCT
  CAGCAACGGGAAGCTGTGCGGGGCAGGGGAGCCCGACCAGCTCGCGCAGAGACTGGCGCAGGAGGCCGCC
  CTGGTGGCCCGCGGCTGCACGCACATCCGCGTCTGCCCCGAGTGCCGCCGCTTCCAGGGCCTGCGGGCAC
  GGCCGTCGCCCGCCCGCAGCGAGGAGAGCCTGGAGTGGGAGAAAACCACCAACAGCAGCGAGCCCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214488 representing NM_014619
  Red=Cloning site Green=Tags(s)
  
  MPRVSAPLVLLPAWLVMVACSPHSLRIAAILDDPMECSRGERLSITLAKNRINRAPERLGKAKVEVDIFE
  LLRDSEYETAETMCQILPKGVVAVLGPSSSPASSSIISNICGEKEVPHFKVAPEEFVKFQFQRFTTLNLH
  PSNTDISVAVAGILNFFNCTTACLICAKAECLLNLEKLLRQFLISKDTLSVRMLDDTRDPTPLLKEIRDD
  KTATIIIHANASMSHTILLKAAELGMVSAYYTYIFTNLEFSLQRMDSLVDDRVNILGFSIFNQSHAFFQE
  FAQSLNQSWQENCDHVPFTGPALSSALLFDAVYAVVTAVQELNRSQEIGVKPLSCGSAQIWQHGTSLMNY
  LRMVELEGLTGHIEFNSKGQRSNYALKILQFTRNGFRQIGQWHVAEGLSMDSHLYASNISDTLFNTTLVV
  TTILENPYLMLKGNHQEMEGNDRYEGFCVDMLKELAEILRFNYKIRLVGDGVYGVPEANGTWTGMVGELI
  ARKADLAVAGLTITAEREKVIDFSKPFMTLGISILYRVHMGRKPGYFSFLDPFSPGVWLFMLLAYLAVSC
  VLFLVARLTPYEWYSPHPCAQGRCNLLVNQYSLGNSLWFPVGGFMQQGSTIAPRALSTRCVSGVWWAFTL
  IIISSYTANLAAFLTVQRMDVPIESVDDLADQTAIEYGTIHGGSSMTFFQNSRYQTYQRMWNYMYSKQPS
  VFVKSTEEGIARVLNSNYAFLLESTMNEYYRQRNCNLTQIGGLLDTKGYGIGMPVGSVFRDEFDLAILQL
  QENNRLEILKRKWWEGGKCPKEEDHRAKGLGMENIGGIFVVLICGLIVAIFMAMLEFLWTLRHSEATEVS
  VCQEMVTELRSIILCQDSIHPRRRRAAVPPPRRPIPEERRPRGTATLSNGKLCGAGEPDQLAQRLAQEAA
  LVARGCTHIRVCPECRRFQGLRARPSPARSEESLEWEKTTNSSEPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014619
• ORF Size: 2868 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014619.1, NM_014619.2, NM_014619.3, NM_014619.4, NP_055434.2
• RefSeq Size: 2871 bp
• RefSeq ORF: 2871 bp
• Locus ID: 2900
• Cytogenetics: 11q23.3
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 107.3 kDa
• Gene Summary: 'This gene encodes a protein that belongs to the glutamate-gated ionic channel family. Glutamate functions as the major excitatory neurotransmitter in the central nervous system through activation of ligand-gated ion channels and G protein-coupled membrane receptors. The protein encoded by this gene forms functional heteromeric kainate-preferring ionic channels with the subunits encoded by related gene family members. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]'