MGAT5B (NM_144677) Human Tagged ORF Clone

CAT#: RC214591

MGAT5B (Myc-DDK-tagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,6-N-acetyl-glucosaminyltransferase, isozyme B (MGAT5B), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MGAT5B
• Synonyms: GnT-IX; GnT-VB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214591 representing NM_144677
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCACCGTCAACCCCGATGGGAAGATAATGGTCAGAAGATGCCTGGTCACCCTGAGACCCTTTCGGC
  TTTTTGTCCTGGGCATCGGCTTCTTCACTCTCTGCTTCCTGATGACGTCTCTGGGAGGCCAGTTCTCGGC
  CCGGCGCCTGGGGGACTCGCCATTCACCATCCGCACAGAAGTGATGGGGGGCCCCGAGTCCCGCGGCGTC
  CTGCGCAAGATGAGCGACCTGCTGGAGCTGATGGTGAAGCGCATGGACGCACTGGCCAGGCTGGAGAACA
  GCAGTGAGCTGCACCGGGCCGGCGGCGACCTGCACTTTCCCGCAGACAGGATGCCCCCTGGGGCCGGCCT
  CATGGAGCGGATCCAGGCTATTGCCCAGAACGTCTCCGACATCGCTGTGAAGGTGGACCAGATCCTGCGC
  CACAGTCTGCTCCTGCACAGCAAGGTGTCAGAAGGCCGGCGGGACCAGTGTGAGGCACCCAGTGACCCCA
  AGTTCCCTGACTGCTCAGGGAAGGTGGAGTGGATGCGTGCCCGCTGGACCTCTGACCCCTGCTACGCCTT
  CTTTGGGGTGGACGGCACCGAGTGCTCCTTCCTCATCTACCTCAGTGAGGTCGAGTGGTTCTGCCCCCCG
  CTGCCCTGGAGGAACCAGACGGCTGCCCAGAGGGCACCCAAGCCCCTCCCCAAAGTCCAGGCAGTTTTCC
  GAAGCAACCTGTCCCACCTTCTGGACCTGATGGGCAGCGGGAAGGAGTCCCTGATCTTCATGAAGAAGCG
  GACCAAGAGGCTCACAGCCCAGTGGGCGCTGGCTGCCCAGCGCCTGGCACAGAAGCTGGGGGCCACCCAG
  AGGGACCAGAAGCAGATCCTGGTCCACATCGGCTTCCTGACGGAGGAGTCCGGGGACGTGTTCAGCCCTC
  GGGTCCTGAAGGGCGGGCCCCTAGGGGAGATGGTGCAGTGGGCGGACATTCTGACTGCACTCTATGTCCT
  GGGCCATGGCCTGCGGGTCACAGTCTCCCTGAAGGAGCTGCAGAGTAACTTAGGGGTACCGCCAGGCCGG
  GGAAGCTGCCCGCTCACCATGCCCCTGCCCTTCGACCTCATCTACACCGACTACCACGGCCTGCAGCAGA
  TGAAGCGGCACATGGGACTCTCCTTCAAGAAGTACCGGTGCCGAATCAGGGTCATCGACACCTTCGGGAC
  GGAACCTGCGTACAACCACGAGGAGTACGCCACGCTGCACGGCTACCGGACCAACTGGGGCTACTGGAAC
  CTCAACCCCAAGCAGTTCATGACCATGTTTCCTCATACCCCCGACAACTCCTTCATGGGCTTCGTGTCCG
  AGGAGCTCAACGAGACGGAGAAGCGGCTCATCAAAGGCGGCAAGGCCAGCAACATGGCCGTGGTGTACGG
  CAAGGAGGCGAGCATCTGGAAGGGGAAGGAGAAGTTCCTGGGCATCCTGAACAAATACATGGAGATCCAT
  GGCACCGTGTACTACGAGAGCCAGCGGCCCCCCGAGGTGCCAGCCTTTGTGAAGAACCACGGCCTCTTAC
  CGCAGCCTGAGTTTCAGCAGCTGCTGCGCAAGGCCAAACTCTTCATCGGGTTTGGCTTCCCCTACGAGGG
  CCCCGCCCCCCTGGAGGCCATCGCCAATGGTTGCATCTTCCTGCAGTCCCGCTTCAGCCCGCCCCACAGC
  TCCCTCAACCACGAGTTCTTCCGAGGCAAGCCCACCTCCAGAGAGGTGTTCTCCCAGCATCCCTACGCGG
  AGAACTTCATCGGCAAGCCCCACGTGTGGACAGTCGACTACAACAACTCAGAGGAGTTTGAAGCAGCCAT
  CAAGGCCATTATGAGAACTCAGGTAGACCCCTACCTACCCTACGAGTACACCTGCGAGGGGATGCTGGAG
  CGGATCCACGCCTACATCCAGCACCAGGACTTCTGCAGAGCTCCAGACCCTGCCCTACCAGAGGCCCACG
  CCCCGCAGAGCCCCTTTGTCCTGGCCCCCAATGCCACCCACCTCGAGTGGGCTCGGAACACCAGCTTGGC
  TCCTGGGGCCTGGCCCCCCGCGCACGCCCTGCGGGCCTGGCTGGCCGTGCCTGGGAGGGCCTGCACCGAC
  ACCTGCCTGGACCACGGGCTAATCTGTGAGCCCTCCTTCTTCCCCTTCCTGAACAGCCAGGACGCCTTCC
  TCAAGCTGCAGGTGCCCTGTGACAGCACCGAGTCGGAGATGAACCACCTGTACCCGGCGTTCGCCCAGCC
  TGGCCAGGAGTGCTACCTGCAGAAGGAGCCTCTGCTCTTCAGCTGCGCCGGCTCCAACACCAAGTACCGC
  CGGCTCTGCCCCTGCCGCGACTTCCGCAAGGGCCAGGTGGCCTTGTGCCAGGGCTGTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214591 representing NM_144677
  Red=Cloning site Green=Tags(s)
  
  MITVNPDGKIMVRRCLVTLRPFRLFVLGIGFFTLCFLMTSLGGQFSARRLGDSPFTIRTEVMGGPESRGV
  LRKMSDLLELMVKRMDALARLENSSELHRAGGDLHFPADRMPPGAGLMERIQAIAQNVSDIAVKVDQILR
  HSLLLHSKVSEGRRDQCEAPSDPKFPDCSGKVEWMRARWTSDPCYAFFGVDGTECSFLIYLSEVEWFCPP
  LPWRNQTAAQRAPKPLPKVQAVFRSNLSHLLDLMGSGKESLIFMKKRTKRLTAQWALAAQRLAQKLGATQ
  RDQKQILVHIGFLTEESGDVFSPRVLKGGPLGEMVQWADILTALYVLGHGLRVTVSLKELQSNLGVPPGR
  GSCPLTMPLPFDLIYTDYHGLQQMKRHMGLSFKKYRCRIRVIDTFGTEPAYNHEEYATLHGYRTNWGYWN
  LNPKQFMTMFPHTPDNSFMGFVSEELNETEKRLIKGGKASNMAVVYGKEASIWKGKEKFLGILNKYMEIH
  GTVYYESQRPPEVPAFVKNHGLLPQPEFQQLLRKAKLFIGFGFPYEGPAPLEAIANGCIFLQSRFSPPHS
  SLNHEFFRGKPTSREVFSQHPYAENFIGKPHVWTVDYNNSEEFEAAIKAIMRTQVDPYLPYEYTCEGMLE
  RIHAYIQHQDFCRAPDPALPEAHAPQSPFVLAPNATHLEWARNTSLAPGAWPPAHALRAWLAVPGRACTD
  TCLDHGLICEPSFFPFLNSQDAFLKLQVPCDSTESEMNHLYPAFAQPGQECYLQKEPLLFSCAGSNTKYR
  RLCPCRDFRKGQVALCQGCL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144677
• ORF Size: 2370 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_144677.1, NM_144677.2, NP_653278.2
• RefSeq Size: 4246
• RefSeq ORF: 2373
• Locus ID: 146664
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 89.1 kDa
• Gene Summary: The MGAT5B gene encodes a beta-1,6-N-acetylglucosaminyltransferase (EC 2.4.1.155) that functions in the synthesis of complex cell surface N-glycans (Kaneko et al., 2003 [PubMed 14623122]). [supplied by OMIM, Nov 2008]