Enterokinase (TMPRSS15) (NM_002772) Human Tagged ORF Clone
CAT#: RC214658
TMPRSS15 (Myc-DDK-tagged)-Human transmembrane protease, serine 15 (TMPRSS15)
"NM_002772" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | TMPRSS15 |
Synonyms | ENTK; PRSS7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC214658 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGTCGAAAAGAGGCATATCTTCTAGGCATCATTCTCTCAGCTCCTATGAAATCATGTTTGCAGCTC TCTTTGCCATATTGGTAGTGCTCTGTGCTGGATTAATTGCAGTATCCTGCCTGACAATCAAGGAATCCCA ACGAGGTGCAGCACTTGGACAGAGTCATGAAGCCAGAGCGACATTTAAAATAACATCCGGAGTTACATAT AATCCTAATTTGCAAGACAAACTCTCAGTGGATTTCAAAGTTCTTGCTTTTGACCTTCAGCAAATGATAG ATGAGATCTTTCTATCAAGCAATCTGAAGAATGAATATAAGAACTCAAGAGTTTTACAATTTGAAAATGG CAGCATTATAGTCGTATTTGACCTTTTCTTTGCCCAGTGGGTGTCAGATCAAAATGTAAAAGAAGAACTG ATTCAAGGCCTTGAAGCAAATAAATCCAGCCAACTGGTCACTTTCCATATTGATTTGAACAGCGTTGATA TCCTAGACAAGCTAACAACCACCAGTCATCTGGCAACTCCAGGAAATGTCTCAATAGAGTGCCTGCCTGG TTCAAGTCCTTGTACTGATGCTCTAACGTGTATAAAAGCTGATTTATTTTGTGATGGAGAAGTAAACTGT CCAGATGGTTCTGACGAAGACAATAAAATGTGTGCCACAGTTTGTGATGGAAGATTTTTGTTAACTGGAT CATCTGGGTCTTTCCAGGCTACTCATTATCCAAAACCTTCTGAAACAAGTGTTGTCTGCCAGTGGATCAT ACGTGTAAACCAAGGACTTTCCATTAAACTGAGCTTCGATGATTTTAATACATATTATACAGATATATTA GATATTTATGAAGGTGTAGGATCAAGCAAGATTTTAAGAGCTTCTATTTGGGAAACTAATCCTGGCACAA TAAGAATTTTTTCCAACCAAGTTACTGCCACCTTTCTTATAGAATCTGATGAAAGTGATTATGTTGGCTT TAATGCAACATATACTGCATTTAACAGCAGTGAGCTTAATAATTATGAGAAAATTAATTGTAACTTTGAG GATGGCTTTTGTTTCTGGGTCCAGGATCTAAATGATGATAATGAATGGGAAAGGATTCAGGGAAGCACCT TTTCTCCTTTTACTGGACCCAATTTTGACCACACTTTTGGCAATGCTTCAGGATTTTACATTTCTACCCC AACTGGACCAGGAGGGAGACAAGAACGAGTGGGGCTTTTAAGCCTCCCTTTGGACCCCACTTTGGAGCCA GCTTGCCTTAGTTTCTGGTATCATATGTATGGTGAAAATGTCCATAAATTAAGCATTAATATCAGCAATG ACCAAAATATGGAGAAGACAGTTTTCCAAAAGGAAGGAAATTATGGAGACAATTGGAATTATGGACAAGT AACCCTAAATGAAACAGTTAAATTTAAGGTTGCTTTTAATGCTTTTAAAAACAAGATCCTGAGTGATATT GCATTGGATGACATTAGCCTAACATATGGGATTTGCAATGGGAGTCTTTATCCAGAACCAACTTTGGTGC CAACTCCTCCACCAGAACTTCCTACGGACTGTGGAGGACCTTTTGAGCTGTGGGAGCCAAATACAACATT CAGTTCTACGAACTTTCCAAACAGCTACCCTAATCTGGCTTTCTGTGTTTGGATTTTAAATGCACAAAAA GGAAAGAATATACAACTTCATTTTCAAGAATTTGACTTAGAAAATATTAACGATGTAGTTGAAATAAGAG ATGGTGAAGAAGCTGATTCCTTGCTCTTAGCTGTGTACACAGGGCCTGGCCCAGTAAAGGATGTGTTCTC TACCACCAACAGAATGACTGTGCTTCTCATCACTAACGATGTGTTGGCAAGAGGAGGGTTTAAAGCAAAC TTTACTACTGGCTATCACTTGGGGATTCCAGAGCCATGCAAGGCAGACCATTTTCAATGTAAAAATGGAG AGTGTGTTCCACTGGTGAATCTCTGTGACGGTCATCTGCACTGTGAGGATGGCTCAGATGAAGCAGATTG TGTGCGTTTTTTCAATGGCACAACGAACAACAATGGTTTAGTGCGGTTCAGAATCCAGAGCATATGGCAT ACAGCTTGTGCTGAGAACTGGACCACCCAGATTTCAAATGATGTTTGTCAACTGCTGGGACTAGGGAGTG GAAACTCATCAAAGCCAATCTTCTCTACCGATGGTGGACCATTTGTCAAATTAAACACAGCACCTGATGG CCACTTAATACTAACACCCAGTCAACAGTGTTTACAGGATTCCTTGATTCGGTTACAGTGTAACCATAAA TCTTGTGGAAAAAAACTGGCAGCTCAAGACATCACCCCAAAGATTGTTGGAGGAAGTAATGCCAAAGAAG GGGCCTGGCCCTGGGTTGTGGGTCTGTATTATGGCGGCCGACTGCTCTGCGGCGCATCTCTCGTCAGCAG TGACTGGCTGGTGTCCGCCGCACACTGCGTGTATGGGAGAAACTTAGAGCCATCCAAGTGGACAGCAATC CTAGGCCTGCATATGAAATCAAATCTGACCTCTCCTCAAACAGTCCCTCGATTAATAGATGAAATTGTCA TAAACCCTCATTACAATAGGCGAAGAAAGGACAACGACATTGCCATGATGCATCTGGAATTTAAAGTGAA TTACACAGATTACATACAACCTATTTGTTTACCGGAAGAAAATCAAGTTTTTCCTCCAGGAAGAAATTGT TCTATTGCTGGTTGGGGGACGGTTGTATATCAAGGTACTACTGCAAACATATTGCAAGAAGCTGATGTTC CTCTTCTATCAAATGAGAGATGCCAACAGCAGATGCCAGAATATAACATTACTGAAAATATGATATGTGC AGGCTATGAAGAAGGAGGAATAGATTCTTGTCAGGGGGATTCAGGAGGACCATTAATGTGCCAAGAAAAC AACAGGTGGTTCCTTGCTGGTGTGACCTCATTTGGATACAAGTGTGCCCTGCCTAATCGCCCCGGAGTGT ATGCCAGGGTCTCAAGGTTTACCGAATGGATACAAAGTTTTCTACAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC214658 protein sequence
Red=Cloning site Green=Tags(s) MGSKRGISSRHHSLSSYEIMFAALFAILVVLCAGLIAVSCLTIKESQRGAALGQSHEARATFKITSGVTY NPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDQNVKEEL IQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNC PDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDIL DIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFE DGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEP ACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDI ALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQK GKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKAN FTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWH TACAENWTTQISNDVCQLLGLGSGNSSKPIFSTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHK SCGKKLAAQDITPKIVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAI LGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNC SIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQEN NRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002772 |
ORF Size | 3057 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002772.1, NM_002772.2, NP_002763.1 |
RefSeq Size | 3946 bp |
RefSeq ORF | 3060 bp |
Locus ID | 5651 |
Cytogenetics | 21q21.1 |
Protein Families | Druggable Genome, Transmembrane |
MW | 112.9 kDa |
Gene Summary | 'This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC303241 | TMPRSS15 (untagged)-Human transmembrane protease, serine 15 (TMPRSS15) |
USD 1,100.00 |
|
RG214658 | TMPRSS15 (GFP-tagged) - Human transmembrane protease, serine 15 (TMPRSS15) |
USD 1,180.00 |
|
RC214658L1 | Lenti ORF clone of Human transmembrane protease, serine 15 (TMPRSS15), Myc-DDK-tagged |
USD 1,270.00 |
|
RC214658L2 | Lenti ORF clone of Human transmembrane protease, serine 15 (TMPRSS15), mGFP tagged |
USD 1,572.00 |
|
RC214658L3 | Lenti ORF clone of Human transmembrane protease, serine 15 (TMPRSS15), Myc-DDK-tagged |
USD 1,270.00 |
|
RC214658L4 | Lenti ORF clone of Human transmembrane protease, serine 15 (TMPRSS15), mGFP tagged |
USD 1,270.00 |
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