Enterokinase (TMPRSS15) (NM_002772) Human Tagged ORF Clone

CAT#: RC214658

TMPRSS15 (Myc-DDK-tagged)-Human transmembrane protease, serine 15 (TMPRSS15)

Product Images

Western blot validation of overexpression lysate (Cat# LY419121) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214658 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMPRSS15
• Synonyms: ENTK; PRSS7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214658 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTCGAAAAGAGGCATATCTTCTAGGCATCATTCTCTCAGCTCCTATGAAATCATGTTTGCAGCTC
  TCTTTGCCATATTGGTAGTGCTCTGTGCTGGATTAATTGCAGTATCCTGCCTGACAATCAAGGAATCCCA
  ACGAGGTGCAGCACTTGGACAGAGTCATGAAGCCAGAGCGACATTTAAAATAACATCCGGAGTTACATAT
  AATCCTAATTTGCAAGACAAACTCTCAGTGGATTTCAAAGTTCTTGCTTTTGACCTTCAGCAAATGATAG
  ATGAGATCTTTCTATCAAGCAATCTGAAGAATGAATATAAGAACTCAAGAGTTTTACAATTTGAAAATGG
  CAGCATTATAGTCGTATTTGACCTTTTCTTTGCCCAGTGGGTGTCAGATCAAAATGTAAAAGAAGAACTG
  ATTCAAGGCCTTGAAGCAAATAAATCCAGCCAACTGGTCACTTTCCATATTGATTTGAACAGCGTTGATA
  TCCTAGACAAGCTAACAACCACCAGTCATCTGGCAACTCCAGGAAATGTCTCAATAGAGTGCCTGCCTGG
  TTCAAGTCCTTGTACTGATGCTCTAACGTGTATAAAAGCTGATTTATTTTGTGATGGAGAAGTAAACTGT
  CCAGATGGTTCTGACGAAGACAATAAAATGTGTGCCACAGTTTGTGATGGAAGATTTTTGTTAACTGGAT
  CATCTGGGTCTTTCCAGGCTACTCATTATCCAAAACCTTCTGAAACAAGTGTTGTCTGCCAGTGGATCAT
  ACGTGTAAACCAAGGACTTTCCATTAAACTGAGCTTCGATGATTTTAATACATATTATACAGATATATTA
  GATATTTATGAAGGTGTAGGATCAAGCAAGATTTTAAGAGCTTCTATTTGGGAAACTAATCCTGGCACAA
  TAAGAATTTTTTCCAACCAAGTTACTGCCACCTTTCTTATAGAATCTGATGAAAGTGATTATGTTGGCTT
  TAATGCAACATATACTGCATTTAACAGCAGTGAGCTTAATAATTATGAGAAAATTAATTGTAACTTTGAG
  GATGGCTTTTGTTTCTGGGTCCAGGATCTAAATGATGATAATGAATGGGAAAGGATTCAGGGAAGCACCT
  TTTCTCCTTTTACTGGACCCAATTTTGACCACACTTTTGGCAATGCTTCAGGATTTTACATTTCTACCCC
  AACTGGACCAGGAGGGAGACAAGAACGAGTGGGGCTTTTAAGCCTCCCTTTGGACCCCACTTTGGAGCCA
  GCTTGCCTTAGTTTCTGGTATCATATGTATGGTGAAAATGTCCATAAATTAAGCATTAATATCAGCAATG
  ACCAAAATATGGAGAAGACAGTTTTCCAAAAGGAAGGAAATTATGGAGACAATTGGAATTATGGACAAGT
  AACCCTAAATGAAACAGTTAAATTTAAGGTTGCTTTTAATGCTTTTAAAAACAAGATCCTGAGTGATATT
  GCATTGGATGACATTAGCCTAACATATGGGATTTGCAATGGGAGTCTTTATCCAGAACCAACTTTGGTGC
  CAACTCCTCCACCAGAACTTCCTACGGACTGTGGAGGACCTTTTGAGCTGTGGGAGCCAAATACAACATT
  CAGTTCTACGAACTTTCCAAACAGCTACCCTAATCTGGCTTTCTGTGTTTGGATTTTAAATGCACAAAAA
  GGAAAGAATATACAACTTCATTTTCAAGAATTTGACTTAGAAAATATTAACGATGTAGTTGAAATAAGAG
  ATGGTGAAGAAGCTGATTCCTTGCTCTTAGCTGTGTACACAGGGCCTGGCCCAGTAAAGGATGTGTTCTC
  TACCACCAACAGAATGACTGTGCTTCTCATCACTAACGATGTGTTGGCAAGAGGAGGGTTTAAAGCAAAC
  TTTACTACTGGCTATCACTTGGGGATTCCAGAGCCATGCAAGGCAGACCATTTTCAATGTAAAAATGGAG
  AGTGTGTTCCACTGGTGAATCTCTGTGACGGTCATCTGCACTGTGAGGATGGCTCAGATGAAGCAGATTG
  TGTGCGTTTTTTCAATGGCACAACGAACAACAATGGTTTAGTGCGGTTCAGAATCCAGAGCATATGGCAT
  ACAGCTTGTGCTGAGAACTGGACCACCCAGATTTCAAATGATGTTTGTCAACTGCTGGGACTAGGGAGTG
  GAAACTCATCAAAGCCAATCTTCTCTACCGATGGTGGACCATTTGTCAAATTAAACACAGCACCTGATGG
  CCACTTAATACTAACACCCAGTCAACAGTGTTTACAGGATTCCTTGATTCGGTTACAGTGTAACCATAAA
  TCTTGTGGAAAAAAACTGGCAGCTCAAGACATCACCCCAAAGATTGTTGGAGGAAGTAATGCCAAAGAAG
  GGGCCTGGCCCTGGGTTGTGGGTCTGTATTATGGCGGCCGACTGCTCTGCGGCGCATCTCTCGTCAGCAG
  TGACTGGCTGGTGTCCGCCGCACACTGCGTGTATGGGAGAAACTTAGAGCCATCCAAGTGGACAGCAATC
  CTAGGCCTGCATATGAAATCAAATCTGACCTCTCCTCAAACAGTCCCTCGATTAATAGATGAAATTGTCA
  TAAACCCTCATTACAATAGGCGAAGAAAGGACAACGACATTGCCATGATGCATCTGGAATTTAAAGTGAA
  TTACACAGATTACATACAACCTATTTGTTTACCGGAAGAAAATCAAGTTTTTCCTCCAGGAAGAAATTGT
  TCTATTGCTGGTTGGGGGACGGTTGTATATCAAGGTACTACTGCAAACATATTGCAAGAAGCTGATGTTC
  CTCTTCTATCAAATGAGAGATGCCAACAGCAGATGCCAGAATATAACATTACTGAAAATATGATATGTGC
  AGGCTATGAAGAAGGAGGAATAGATTCTTGTCAGGGGGATTCAGGAGGACCATTAATGTGCCAAGAAAAC
  AACAGGTGGTTCCTTGCTGGTGTGACCTCATTTGGATACAAGTGTGCCCTGCCTAATCGCCCCGGAGTGT
  ATGCCAGGGTCTCAAGGTTTACCGAATGGATACAAAGTTTTCTACAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214658 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGSKRGISSRHHSLSSYEIMFAALFAILVVLCAGLIAVSCLTIKESQRGAALGQSHEARATFKITSGVTY
  NPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDQNVKEEL
  IQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNC
  PDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDIL
  DIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFE
  DGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEP
  ACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDI
  ALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQK
  GKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKAN
  FTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWH
  TACAENWTTQISNDVCQLLGLGSGNSSKPIFSTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHK
  SCGKKLAAQDITPKIVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAI
  LGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNC
  SIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQEN
  NRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002772
• ORF Size: 3057 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002772.1, NM_002772.2, NP_002763.1
• RefSeq Size: 3946 bp
• RefSeq ORF: 3060 bp
• Locus ID: 5651
• Cytogenetics: 21q21.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 112.9 kDa
• Gene Summary: 'This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]'