MYBPC1 (NM_206819) Human Tagged ORF Clone

CAT#: RC214729

MYBPC1 (Myc-DDK-tagged)-Human myosin binding protein C, slow type (MYBPC1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MYBPC1
• Synonyms: LCCS4; MYBPCC; MYBPCS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214729 representing NM_206819
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGAACCCACTAAGAAAGAGGAAAATGAAGTGCCAGCCCCAGCCCCACCCCCGGAAGAACCAAGTA
  AAGAGAAGGAGGCCGGAACTACACCAGCAAAAGATGAAGAGGAAGTCTCCCCGCCTAGCGCCTTGCCTCC
  AGGTTTGGGTAGTCGGGCCCTGGAGAGAAAAGATTCAGACTGGACCCTTGTCGAAACTCCTCCTGGGGAG
  GAACAAGCCAAGCAGAATGCCAACTCCCAGCTGTCCATCTTGTTCATTGAAAAACCTCAAGGAGGAACAG
  TGAAAGTTGGTGAAGATATCACCTTCATAGCCAAAGTCAAGGCTGAAGATCTTCTGAGAAAACCCACTAT
  CAAATGGTTCAAAGGAAAATGGATGGACCTGGCCAGCAAAGCCGGGAAGCACCTTCAGCTGAAGGAAACC
  TTTGAGAGGCACAGTCGGGTGTACACATTTGAGATGCAGATCATCAAGGCCAAAGATAACTTTGCAGGAA
  ATTACAGATGCGAGGTCACCTATAAGGATAAGTTTGACAGCTGTTCATTTGATCTTGAAGTGCACGAATC
  TACTGGGACTACTCCAAACATTGACATCAGATCTGCTTTCAAGAGAAGTGGAGAAGGTCAAGAGGATGCA
  GGAGAACTTGACTTTAGTGGTCTCCTGAAACGTAGGGAGGTGAAGCAGCAGGAGGAAGAACCCCAGGTGG
  ACGTATGGGAGTTGCTGAAGAACGCGAAACCCAGTGAGTACGAGAAGATCGCCTTCCAGTATGGAATCAC
  CGACCTGCGCGGCATGCTCAAGCGACTCAAGCGCATGCGCAGAGAGGAGAAGAAGAGCGCAGCTTTTGCA
  AAAATTCTTGATCCTGCATATCAGGTTGACAAAGGAGGCAGAGTGAGGTTTGTTGTGGAGCTGGCAGATC
  CAAAGTTGGAGGTGAAATGGTATAAAAATGGTCAAGAAATTCGACCCAGTACCAAATACATCTTTGAACA
  CAAAGGATGCCAGAGAATCCTGTTTATCAATAACTGTCAGATGACAGATGATTCAGAGTATTATGTGACA
  GCCGGTGATGAGAAATGTTCCACTGAGCTCTTCGTAAGAGAGCCTCCAATTATGGTGACCAAACAGCTGG
  AAGATACAACTGCTTATTGTGGGGAGAGAGTGGAATTAGAATGTGAGGTGTCTGAAGATGATGCCAATGT
  AAAATGGTTTAAGAATGGTGAAGAGATTATCCCTGGTCCAAAATCAAGATACCGAATTAGAGTTGAGGGT
  AAAAAACACATCTTGATCATAGAGGGAGCAACAAAGGCTGATGCTGCAGAATATTCAGTAATGACAACAG
  GAGGACAATCATCTGCTAAACTTAGTGTTGACTTGAAACCTCTGAAGATTTTGACACCTCTGACTGATCA
  GACTGTAAATCTTGGAAAAGAAATCTGCCTGAAGTGTGAAATCTCTGAAAACATACCAGGAAAATGGACT
  AAAAATGGCCTACCTGTTCAGGAGAGTGACCGTCTAAAGGTGGTTCACAAGGGAAGGATCCACAAGTTAG
  TGATAGCCAATGCCCTCACTGAAGATGAAGGTGATTATGTATTTGCACCTGATGCCTACAATGTTACTCT
  GCCTGCCAAAGTTCATGTTATTGATCCTCCTAAGATCATCCTGGATGGTCTTGATGCTGACAACACAGTG
  ACAGTGATTGCAGGAAACAAGCTTCGTCTTGAGATCCCCATCAGCGGAGAACCACCTCCTAAAGCCATGT
  GGAGCCGGGGAGATAAGGCTATTATGGAAGGCAGTGGCCGGATAAGAACAGAATCTTACCCTGATAGCAG
  CACTCTGGTCATTGATATAGCTGAAAGAGATGACTCTGGTGTTTACCACATCAATCTGAAAAACGAAGCT
  GGAGAGGCACATGCAAGCATCAAGGTTAAAGTTGTGGACTTCCCTGATCCTCCAGTGGCACCGACTGTGA
  CAGAGGTGGGAGATGACTGGTGTATCATGAACTGGGAGCCTCCTGCCTACGACGGAGGCTCTCCAATCCT
  AGGATATTTTATTGAGAGGAAGAAGAAACAAAGCTCCAGGTGGATGAGGCTGAATTTTGATCTCTGCAAA
  GAAACAACTTTTGAGCCCAAGAAGATGATTGAAGGTGTGGCCTATGAGGTCCGCATCTTTGCAGTCAATG
  CCATTGGCATCTCCAAGCCCAGTATGCCCTCCAGGCCTTTTGTTCCTTTGGCTGTAACAAGCCCTCCTAC
  TCTTCTGACTGTGGACTCTGTCACTGACACGACTGTCACGATGAGGTGGCGCCCCCCAGACCACATTGGT
  GCAGCAGGTTTAGATGGCTATGTGCTAGAGTATTGCTTTGAAGGAACTGAGGACTGGATAGTTGCAAACA
  AAGATCTGATTGACAAGACGAAGTTCACCATCACAGGTCTGCCAACAGATGCAAAGATCTTTGTGCGTGT
  GAAGGCTGTTAATGCAGCTGGTGCCAGCGAGCCCAAGTACTATTCTCAGCCCATTCTCGTGAAGGAAATC
  ATAGAACCTCCAAAGATTCGCATTCCAAGACACCTGAAGCAAACCTATATCCGCAGAGTTGGAGAAGCTG
  TCAATCTGGTTATACCTTTCCAGGGAAAACCAAGACCAGAATTAACTTGGAAGAAGGATGGTGCAGAAAT
  TGATAAGAATCAAATAAACATTCGCAACTCTGAGACTGATACAATCATATTTATTAGAAAAGCAGAGAGG
  AGCCACTCTGGGAAATATGATCTGCAAGTCAAAGTGGACAAATTCGTGGAGACCGCATCAATTGACATCC
  AGATCATTGACCGTCCAGGTCCACCCCAAATTGTGAAGATTGAGGATGTCTGGGGAGAAAATGTCGCTCT
  CACATGGACTCCACCAAAGGATGATGGAAATGCTGCTATCACAGGCTATACCATTCAGAAGGCTGACAAG
  AAGAGCATGGAATGGTTTACTGTCATTGAGCATTATCATCGAACCAGTGCCACCATTACTGAATTGGTCA
  TAGGGAATGAATATTACTTCCGGGTCTTTTCTGAAAACATGTGTGGCCTCAGTGAGGATGCCACCATGAC
  TAAAGAGAGTGCAGTGATCGCCAGGGATGGTAAAATCTACAAAAATCCAGTGTATGAAGACTTTGATTTC
  TCAGAGGCACCCATGTTTACTCAGCCTTTGGTTAACACCTATGCCATAGCTGGTTACAATGCCACCCTAA
  ACTGCAGTGTGAGAGGAAATCCTAAGCCTAAAATAACCTGGATGAAAAACAAAGTTGCTATTGTGGATGA
  TCCAAGATACAGGATGTTCAGCAACCAGGGAGTCTGTACCCTGGAAATTCGCAAGCCCAGCCCCTATGAT
  GGAGGCACTTACTGCTGCAAAGCAGTCAATGACCTTGGGACAGTGGAGATTGAATGCAAACTGGAGGTGA
  AAGTCATTGCACAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214729 representing NM_206819
  Red=Cloning site Green=Tags(s)
  
  MPEPTKKEENEVPAPAPPPEEPSKEKEAGTTPAKDEEEVSPPSALPPGLGSRALERKDSDWTLVETPPGE
  EQAKQNANSQLSILFIEKPQGGTVKVGEDITFIAKVKAEDLLRKPTIKWFKGKWMDLASKAGKHLQLKET
  FERHSRVYTFEMQIIKAKDNFAGNYRCEVTYKDKFDSCSFDLEVHESTGTTPNIDIRSAFKRSGEGQEDA
  GELDFSGLLKRREVKQQEEEPQVDVWELLKNAKPSEYEKIAFQYGITDLRGMLKRLKRMRREEKKSAAFA
  KILDPAYQVDKGGRVRFVVELADPKLEVKWYKNGQEIRPSTKYIFEHKGCQRILFINNCQMTDDSEYYVT
  AGDEKCSTELFVREPPIMVTKQLEDTTAYCGERVELECEVSEDDANVKWFKNGEEIIPGPKSRYRIRVEG
  KKHILIIEGATKADAAEYSVMTTGGQSSAKLSVDLKPLKILTPLTDQTVNLGKEICLKCEISENIPGKWT
  KNGLPVQESDRLKVVHKGRIHKLVIANALTEDEGDYVFAPDAYNVTLPAKVHVIDPPKIILDGLDADNTV
  TVIAGNKLRLEIPISGEPPPKAMWSRGDKAIMEGSGRIRTESYPDSSTLVIDIAERDDSGVYHINLKNEA
  GEAHASIKVKVVDFPDPPVAPTVTEVGDDWCIMNWEPPAYDGGSPILGYFIERKKKQSSRWMRLNFDLCK
  ETTFEPKKMIEGVAYEVRIFAVNAIGISKPSMPSRPFVPLAVTSPPTLLTVDSVTDTTVTMRWRPPDHIG
  AAGLDGYVLEYCFEGTEDWIVANKDLIDKTKFTITGLPTDAKIFVRVKAVNAAGASEPKYYSQPILVKEI
  IEPPKIRIPRHLKQTYIRRVGEAVNLVIPFQGKPRPELTWKKDGAEIDKNQINIRNSETDTIIFIRKAER
  SHSGKYDLQVKVDKFVETASIDIQIIDRPGPPQIVKIEDVWGENVALTWTPPKDDGNAAITGYTIQKADK
  KSMEWFTVIEHYHRTSATITELVIGNEYYFRVFSENMCGLSEDATMTKESAVIARDGKIYKNPVYEDFDF
  SEAPMFTQPLVNTYAIAGYNATLNCSVRGNPKPKITWMKNKVAIVDDPRYRMFSNQGVCTLEIRKPSPYD
  GGTYCCKAVNDLGTVEIECKLEVKVIAQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206819
• ORF Size: 3444 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_206819.1, NM_206819.2, NP_996555.1
• RefSeq Size: 3935 bp
• RefSeq ORF: 3447 bp
• Locus ID: 4604
• Cytogenetics: 12q23.2
• MW: 129.1 kDa
• Gene Summary: 'This gene encodes a member of the myosin-binding protein C family. Myosin-binding protein C family members are myosin-associated proteins found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. The encoded protein is the slow skeletal muscle isoform of myosin-binding protein C and plays an important role in muscle contraction by recruiting muscle-type creatine kinase to myosin filaments. Mutations in this gene are associated with distal arthrogryposis type I. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]'