MRG15 (MORF4L1) (NM_206839) Human Tagged ORF Clone

CAT#: RC214758

MORF4L1 (Myc-DDK-tagged)-Human mortality factor 4 like 1 (MORF4L1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY404237) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214758 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MORF4L1
• Synonyms: Eaf3; FWP006; HsT17725; MEAF3; MORFRG15; MRG15; S863-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214758 representing NM_206839
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGAAGCAGGACCCGAAGCCTAAATTCCAGGAGGGTGAGCGAGTGCTGTGCTTTCATGGGCCTC
  TTCTTTATGAAGCAAAGTGTGTAAAGGTTGCCATAAAGGACAAACAAGTGAAATACTTCATACATTACAG
  TGGTTGGAATAAAAAAAGTGCTGTGAGGCCCAGGCGCTCTGAAAAATCTTTGAAGACACATGAGGATATT
  GTAGCCCTTTTTCCTGTTCCTGAAGGAGCTCCCTCAGTACACCACCCCCTCCTGACCTCTAGTTGGGATG
  AATGGGTTCCGGAGAGCAGAGTACTCAAATACGTGGACACCAATTTGCAGAAACAGCGAGAACTTCAAAA
  AGCCAATCAGGAGCAGTATGCAGAGGGGAAGATGAGAGGGGCTGCCCCAGGAAAGAAGACATCTGGTCTG
  CAACAGAAAAATGTTGAAGTGAAAACGAAAAAGAACAAACAGAAAACACCTGGAAATGGAGATGGTGGCA
  GTACCAGTGAGACCCCTCAGCCTCCTCGGAAGAAAAGGGCCCGGGTAGATCCTACTGTTGAAAATGAGGA
  AACATTCATGAACAGAGTTGAAGTTAAAGTAAAGATTCCTGAAGAGCTAAAACCGTGGCTTGTTGATGAC
  TGGGACTTAATTACCAGGCAAAAACAGCTCTTTTATCTTCCTGCCAAGAAGAATGTGGATTCCATTCTTG
  AGGATTATGCAAATTACAAGAAATCTCGTGGAAACACAGATAATAAGGAGTATGCGGTTAATGAAGTTGT
  GGCAGGGATAAAAGAATACTTCAACGTAATGTTGGGTACCCAGCTACTCTATAAATTTGAGAGACCACAG
  TATGCTGAAATTCTTGCAGATCATCCCGATGCACCCATGTCCCAGGTGTATGGAGCGCCACATCTCCTGA
  GATTATTTGTACGAATTGGAGCAATGTTGGCTTATACACCTCTGGATGAGAAGAGCCTTGCTTTATTACT
  CAATTATCTTCACGATTTCCTAAAGTACCTGGCAAAGAATTCTGCAACTTTGTTCAGTGCCAGCGATTAT
  GAAGTGGCTCCTCCTGAGTACCATCGGAAAGCTGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214758 representing NM_206839
  Red=Cloning site Green=Tags(s)
  
  MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKVAIKDKQVKYFIHYSGWNKKSAVRPRRSEKSLKTHEDI
  VALFPVPEGAPSVHHPLLTSSWDEWVPESRVLKYVDTNLQKQRELQKANQEQYAEGKMRGAAPGKKTSGL
  QQKNVEVKTKKNKQKTPGNGDGGSTSETPQPPRKKRARVDPTVENEETFMNRVEVKVKIPEELKPWLVDD
  WDLITRQKQLFYLPAKKNVDSILEDYANYKKSRGNTDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQ
  YAEILADHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLNYLHDFLKYLAKNSATLFSASDY
  EVAPPEYHRKAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_206839
• ORF Size: 1086 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_206839.1, NM_206839.2, NP_996670.1
• RefSeq Size: 1969
• RefSeq ORF: 1089
• Locus ID: 10933
• Protein Families: Transcription Factors
• MW: 41.3 kDa
• Gene Summary: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage. Also component of the mSin3A complex which acts to repress transcription by deacetylation of nucleosomal histones. Required for homologous recombination repair (HRR) and resistance to mitomycin C (MMC). Involved in the localization of PALB2, BRCA2 and RAD51, but not BRCA1, to DNA-damage foci. [UniProtKB/Swiss-Prot Function]