DNAJC6 (NM_014787) Human Tagged ORF Clone

CAT#: RC214801

DNAJC6 (Myc-DDK-tagged)-Human DnaJ (Hsp40) homolog, subfamily C, member 6 (DNAJC6)


  "NM_014787" in other vectors (4)

Reconstitution Protocol

USD 750.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol DNAJC6
Synonyms DJC6; PARK19
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC214801 representing NM_014787
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAAGATTCTGAAAATAAAGGTGCCTCATCTCCAGACATGGAGCCCAGCTATGGGGGAGGTCTCTTTG
ACATGGTAAAAGGAGGTGCAGGGAGGCTCTTTAGTAACCTAAAGGACAACTTGAAAGACACCCTCAAAGA
CACATCTTCTAGAGTGATACAATCTGTGACCAGCTACACAAAGGGAGATTTAGACTTCACTTATGTTACC
TCCAGAATTATTGTGATGTCCTTTCCTCTGGACAATGTTGACATAGGATTCAGGAATCAGGTTGATGACA
TTCGAAGCTTTTTGGATTCCAGACATCTTGACCACTACACAGTATACAATCTGTCACCTAAGTCTTATCG
AACTGCCAAGTTTCACAGCCGGGTCTCAGAATGCAGTTGGCCCATTAGGCAGGCTCCCAGTCTGCACAAC
CTTTTTGCTGTGTGTCGGAATATGTATAACTGGCTACTGCAGAATCCCAAAAATGTCTGTGTTGTCCACT
GCTTGGATGGACGGGCGGCATCATCAATTCTGGTTGGTGCTATGTTCATTTTCTGTAATCTCTACTCTAC
TCCTGGCCCAGCCATTCGATTGCTATATGCAAAGCGACCAGGAATTGGACTTTCACCATCCCATAGGAGA
TACCTGGGCTATATGTGTGACCTACTGGCAGACAAGCCCTACCGCCCTCACTTCAAGCCTCTCACAATTA
AGTCGATCACTGTCAGTCCAATACCCTTTTTCAACAAACAGAGGAATGGATGTCGCCCTTACTGTGATGT
ACTCATTGGAGAAACCAAAATATATTCGACTTGCACAGATTTTGAACGAATGAAAGAATATCGTGTCCAA
GATGGAAAAATCTTCATTCCCTTGAACATCACTGTGCAAGGAGACGTGGTTGTTTCCATGTATCACTTGA
GGTCAACCATTGGGAGCCGGCTACAGGCTAAGGTGACCAACACACAGATATTCCAGCTTCAGTTTCACAC
TGGATTCATACCACTGGACACAACAGTTTTAAAGTTCACCAAGCCTGAGTTAGATGCATGTGATGTACCA
GAAAAATATCCTCAGCTATTTCAGGTGACACTGGATGTAGAACTACAGCCCCATGACAAAGTAATAGACT
TAACTCCACCATGGGAACATTACTGCACAAAAGATGTCAATCCCAGCATCCTCTTCTCTTCTCACCAGGA
ACATCAAGATACGCTGGCCTTAGGAGGACAGGCTCCAATAGATATCCCTCCAGACAACCCCAGGCATTAC
GGACAAAGTGGTTTCTTTGCCTCTCTCTGTTGGCAAGATCAGAAATCGGAGAAGTCATTCTGTGAGGAGG
ACCACGCTGCCCTAGTGAATCAGGAAAGTGAGCAATCAGATGATGAACTTCTGACACTTTCCAGTCCGCA
TGGCAATGCCAATGGTGACAAGCCTCATGGAGTCAAGAAGCCCAGCAAAAAGCAGCAGGAGCCAGCAGCC
CCTCCACCCCCTGAGGATGTGGACCTTTTGGGCCTGGAAGGGTCTGCAATGAGTAACAGCTTCTCTCCGC
CAGCGGCTCCTCCCACCAATTCTGAACTACTGAGTGACCTGTTTGGGGGTGGAGGTGCAGCTGGTCCCAC
CCAGGCTGGACAGTCAGGAGTGGAAGATGTGTTTCATCCTAGTGGACCTGCGTCTACCCAGTCAACACCA
CGCCGCTCTGCCACCTCCACCTCTGCGTCTCCAACCCTAAGAGTGGGAGAAGGTGCCACCTTTGACCCAT
TTGGAGCACCTTCTAAACCATCAGGTCAGGATTTGCTGGGTTCTTTTCTGAACACATCCAGTGCTTCCAG
TGACCCCTTTCTCCAGCCAACAAGAAGTCCTTCGCCCACAGTACATGCTTCTAGTACGCCTGCTGTGAAC
ATTCAGCCAGATGTTTCTGGAGGTTGGGACTGGCATGCTAAACCAGGAGGCTTTGGAATGGGAAGCAAGT
CAGCTGCCACCAGCCCAACCGGATCCTCGCATGGTACTCCCACCCATCAAAGCAAACCCCAGACTCTGGA
TCCTTTTGCCGACCTTGGGACACTAGGTAGTTCTTCCTTTGCCAGCAAACCCACCACACCAACTGGATTG
GGTGGAGGATTCCCGCCTCTCAGCTCGCCACAGAAGGCGTCTCCCCAGCCTATGGGTGGCGGGTGGCAGC
AGGGAGGTGCCTACAACTGGCAGCAGCCACAGCCTAAGCCTCAGCCCAGCATGCCCCACTCCTCTCCCCA
GAACCGACCCAACTACAACGTGAGCTTCTCAGCCATGCCTGGGGGCCAGAACGAACGTGGGAAAGGATCA
AGTAATTTGGAAGGGAAACAAAAAGCAGCTGATTTTGAAGACCTACTCTCTGGTCAAGGTTTCAATGCTC
ACAAAGACAAAAAGGGGCCTCGGACAATAGCTGAGATGAGAAAGGAGGAAATGGCCAAGGAAATGGATCC
TGAGAAATTAAAGATTCTGGAATGGATTGAAGGCAAAGAAAGAAATATCAGAGCCCTTCTTTCCACGATG
CATACCGTACTATGGGCTGGGGAGACCAAGTGGAAACCAGTTGGCATGGCAGACCTGGTAACACCAGAGC
AGGTGAAGAAGGTGTACAGGAAGGCTGTCCTGGTGGTGCACCCAGATAAAGCTACTGGGCAACCCTATGA
ACAATACGCAAAGATGATTTTCATGGAGCTCAATGATGCCTGGTCTGAATTTGAAAACCAAGGCCAAAAG
CCCTTATAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC214801 representing NM_014787
Red=Cloning site Green=Tags(s)

MKDSENKGASSPDMEPSYGGGLFDMVKGGAGRLFSNLKDNLKDTLKDTSSRVIQSVTSYTKGDLDFTYVT
SRIIVMSFPLDNVDIGFRNQVDDIRSFLDSRHLDHYTVYNLSPKSYRTAKFHSRVSECSWPIRQAPSLHN
LFAVCRNMYNWLLQNPKNVCVVHCLDGRAASSILVGAMFIFCNLYSTPGPAIRLLYAKRPGIGLSPSHRR
YLGYMCDLLADKPYRPHFKPLTIKSITVSPIPFFNKQRNGCRPYCDVLIGETKIYSTCTDFERMKEYRVQ
DGKIFIPLNITVQGDVVVSMYHLRSTIGSRLQAKVTNTQIFQLQFHTGFIPLDTTVLKFTKPELDACDVP
EKYPQLFQVTLDVELQPHDKVIDLTPPWEHYCTKDVNPSILFSSHQEHQDTLALGGQAPIDIPPDNPRHY
GQSGFFASLCWQDQKSEKSFCEEDHAALVNQESEQSDDELLTLSSPHGNANGDKPHGVKKPSKKQQEPAA
PPPPEDVDLLGLEGSAMSNSFSPPAAPPTNSELLSDLFGGGGAAGPTQAGQSGVEDVFHPSGPASTQSTP
RRSATSTSASPTLRVGEGATFDPFGAPSKPSGQDLLGSFLNTSSASSDPFLQPTRSPSPTVHASSTPAVN
IQPDVSGGWDWHAKPGGFGMGSKSAATSPTGSSHGTPTHQSKPQTLDPFADLGTLGSSSFASKPTTPTGL
GGGFPPLSSPQKASPQPMGGGWQQGGAYNWQQPQPKPQPSMPHSSPQNRPNYNVSFSAMPGGQNERGKGS
SNLEGKQKAADFEDLLSGQGFNAHKDKKGPRTIAEMRKEEMAKEMDPEKLKILEWIEGKERNIRALLSTM
HTVLWAGETKWKPVGMADLVTPEQVKKVYRKAVLVVHPDKATGQPYEQYAKMIFMELNDAWSEFENQGQK
PLY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014787
ORF Size 2739 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014787.1, NM_014787.2, NM_014787.3, NP_055602.1
RefSeq Size 5750 bp
RefSeq ORF 2742 bp
Locus ID 9829
Domains DnaJ
Protein Families Druggable Genome
Protein Pathways Endocytosis
MW 99.8 kDa
Gene Summary DNAJC6 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]). [supplied by OMIM, Mar 2008]

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