FHDC1 (NM_033393) Human Tagged ORF Clone

CAT#: RC214806

FHDC1 (Myc-DDK-tagged)-Human FH2 domain containing 1 (FHDC1)

Product Images

Western blot validation of overexpression lysate (Cat# LY409574) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214806 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FHDC1
• Synonyms: KIAA1727
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214806 representing NM_033393
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGTTATGAATTGTGTCTCCTTGGTCAGTGATAAAGAAAATGGGAATATTGCCACAGCACCTGGAT
  TCATGATTGGGCAGACACCTCCTCCAGCACCTCCTCCACCTCCTCCTCCACCCCCTCCATCTCCACCATG
  TTCATGTTCAAGGGAAGAGTGTCCTTCCTCCCCTCCTCCACCCCCACCACCTCCACTTCCTGGGGAGCCT
  CCCATCCCACCTCCCCCACCAGGCCTACCCCCAACTACTCACATGAACGGCTACAGCCACCTTGGTAAGA
  AAAAGCGGATGAGAAGCTTTTTTTGGAAAACTATTCCGGAGGAGCAAGTTCGAGGCAAAACCAACATCTG
  GACCTTGGCAGCCAGGCAGGAACATCACTACCAAATTGATACAAAGACCATTGAGGAGCTCTTTGGGCAG
  CAGGAAGACACCACCAAGTCTTCCCTTCCTAGGAGAGGAAGAACTTTAAATTCATCCTTCAGAGAAGCTC
  GAGAAGAGATTACTATTTTGGATGCAAAACGGAGCATGAACATTGGGATATTTCTTAAGCAATTTAAGAA
  GTCTCCTCGGTCCATTGTAGAAGATATTCATCAAGGAAAAAGTGAGCATTATGGATCAGAGACCTTGCGA
  GAATTTCTTAAGTTTTTGCCAGAGTCAGAAGAGGTAAAGAAGTTAAAAGCGTTTAGTGGCGACGTGTCGA
  AGCTGTCTCTGGCAGATTCCTTTCTGTATGGCTTAATTCAGGTGCCAAACTATTCACTTCGGATTGAAGC
  CATGGTGCTAAAGAAGGAATTTCTACCTTCTTGCTCTTCTCTATATACAGATATAACAGTTTTAAGAACT
  GCTATAAAAGAACTGATGTCATGTGAAGAGCTACATTCAATATTACACTTGGTGCTCCAGGCTGGGAATA
  TCATGAATGCAGGAGGGTATGCCGGCAATGCAGTAGGATTTAAACTGTCTTCTTTGCTCAAATTGGCAGA
  CACAAAAGCAAACAAACCTGGGATGAATCTCCTGCACTTTGTTGCACAGGAAGCCCAAAAGAAAGATACC
  ATTCTTCTAAACTTTTCAGAAAAATTGCATCATGTTCAGAAGACTGCTAGATTATCTCTGGAGAACACGG
  AGGCAGAACTGCACTTGCTGTTTGTCAGGACAAAATCACTAAAAGAAAACATCCAGCGGGATGGTGAACT
  TTGTCAGCAGATGGAAGATTTTCTTCAGTTTGCCATAGAAAAGCTGAGGGAACTGGAATGCTGGAAACAA
  GAGCTCCAGGATGAGGCCTACACCCTTATAGATTTTTTCTGTGAAGACAAAAAAACCATGAAACTGGATG
  AATGCTTTCAGATATTTAGAGATTTCTGTACCAAATTCAACAAAGCAGTTAAGGACAACCACGACCGGGA
  GGCGCAGGAGCTGAGGCAGCTGCAGAGGCTGAAGGAGCAGGAGCAGAAGCAGCGCTCCTGGGCAACTGGG
  GAGCTGGGGGCATTTGGCCGGAGCAGCAGTGAGAATGATGTGGAGCTGCTGACCAAGAAGGGTGCAGAGG
  GCCTGCTCCCTTTCCTGCACCCCAGGCCCATCAGCCCCTCCAGCCCCTCCTACCGGCCCCCGAACACCCG
  CCGCTCCCGCCTCTCCCTGGGTCCCTCTGCTGACCGGGAGCTGCTGACCTTCTTGGAGAGCTCCACCGGC
  AGCCCTGAGGAGCCCAATAAGTTCCACAGCCTGCCCCGGAGCAGCCCCCGGCAGGCCCGGCCCACGATAG
  CCTGCCTGGAGCCTGCAGAAGTGAGGCACCAGGACTCCAGCTTTGCACACAAACCTCAGGCCTCGGGGGG
  CCAGGAGGAGGCCCCCAACCCACCCTCAGCACAGGCGCACCAGCTTGCAGCCGCCCAGCCTGAGAACCAT
  GCCTCTGCCTTCCCCAGAGCTCGGTGCCAGGGCGTCAGTGTCCTCCGAAAGAGGTACAGTGAGCCGGTGA
  GCCTGGGCTCAGCACAGTCCCCTCCTCTCTCGCCATTGGCTCTGGGAATTAAGGAGCATGAGCTGGTGAC
  AGGGCTGGCCCAGTTCAACCTCCAGGGTTCCCAGGGCATGGAGGAGACCTCCCAGCTGACTCTGAGTGAC
  TTCAGCCCGATGGAGCTAGAGTCTGTGGGGCATAGGGGCCCGCAGTCCCTCAGTGCCAGCAGCAGCAGCC
  TGACACCCATGGGCAGAGATGCCCTGGGGAGTCTCAGCCCAGCGCTGGAGGATGGCAAGGCTGCCCCCGA
  TGAGCCTGGAAGTGCAGCTTTGGGATCTGTGGGTAGCAGCGACCCTGAGAACAAAGATCCTAGACCTCTG
  TTCTGCATCTCGGACACCACCGACTGCTCACTGACCCTGGACTGCTCAGAGGGAACCGACTCCAGACCCA
  GAGGCGGGGACCCGGAGGAAGGCGGGGAAGGGGATGGCTCCATGTCCTCTGGGGTTGGAGAAATGGGGGA
  CAGCCAAGTCTCCTCCAACCCTACATCCAGCCCCCCTGGGGAGGCTCCTGCCCCCGTCTCTGTGGATAGT
  GAGCCCAGCTGCAAGGGCGGCCTGCCCAGGGACAAACCCACCAAAAGGAAAGATGTTGTAGCACCAAAGA
  GAGGCTCCCTGAAAGAGGCGTCTCCCGGGGCCTCCAAGCCCGGGAGCGCCCGGCGGAGCCAGGGGGCAGT
  GGCCAAGTCTGTGCGGACCCTGACCGCCTCAGAGAACGAGAGCATGCGCAAGGTCATGCCCATCACCAAG
  TCCAGCAGAGGCGCCGGCTGGAGGCGACCAGAGCTGTCATCCCGGGGGCCCTCCCAGAATCCCCCCAGCA
  GCACAGATACTGTGTGGTCACGCCAGAACTCCGTGCGGAGGGCCTCCACAGGCGCCGAAGAGCAGAGGCT
  GCCGCGGGGGAGCAGCGGCTCCAGCAGCACCCGTCCGGGGAGGGACGTTCCCCTGCAGCCCAGGGGTTCT
  TTCAAGAAGCCCAGTGCCAAACCACTCAGGAACCTCCCCAGACAGAAGCCTGAGGAAAATAAGACCTGCC
  GCGCCCACTCCGAGGGCCCTGAGAGTCCCAAAGAAGAGCCCAAGACCCCGTCAGTGCCCAGCGTCCCCCA
  CGAACTACCCCGTGTCCCGAGCTTTGCCCGGAACACAGTGGCCTCCTCCTCTCGAAGCATGAGAACAGAT
  CTTCCTCCCGTGGCCAAAGCCCCCGGCATCACTCGGACAGTGTCGCAGCGGCAGCTGAGGGTGAAAGGGG
  ACCCCGAGGATGCCGCTCCCAAGGACAGCAGCACTTTGAGGCGAGCCAGCAGTGCCCGGGCCCCCAAGAA
  GCGCCCAGAGTCTGCGGAGGGTCCCAGTGCCAACACGGAGGCCCCTCTGAAGGCCAGAGGGGCTGGGGAA
  AGGGCCTCCCTCCGTCGGAAGGACTCCAGTCGGACCACGCTGGGGAGAATCCTCAATCCCTTACGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214806 representing NM_033393
  Red=Cloning site Green=Tags(s)
  
  MHVMNCVSLVSDKENGNIATAPGFMIGQTPPPAPPPPPPPPPPSPPCSCSREECPSSPPPPPPPPLPGEP
  PIPPPPPGLPPTTHMNGYSHLGKKKRMRSFFWKTIPEEQVRGKTNIWTLAARQEHHYQIDTKTIEELFGQ
  QEDTTKSSLPRRGRTLNSSFREAREEITILDAKRSMNIGIFLKQFKKSPRSIVEDIHQGKSEHYGSETLR
  EFLKFLPESEEVKKLKAFSGDVSKLSLADSFLYGLIQVPNYSLRIEAMVLKKEFLPSCSSLYTDITVLRT
  AIKELMSCEELHSILHLVLQAGNIMNAGGYAGNAVGFKLSSLLKLADTKANKPGMNLLHFVAQEAQKKDT
  ILLNFSEKLHHVQKTARLSLENTEAELHLLFVRTKSLKENIQRDGELCQQMEDFLQFAIEKLRELECWKQ
  ELQDEAYTLIDFFCEDKKTMKLDECFQIFRDFCTKFNKAVKDNHDREAQELRQLQRLKEQEQKQRSWATG
  ELGAFGRSSSENDVELLTKKGAEGLLPFLHPRPISPSSPSYRPPNTRRSRLSLGPSADRELLTFLESSTG
  SPEEPNKFHSLPRSSPRQARPTIACLEPAEVRHQDSSFAHKPQASGGQEEAPNPPSAQAHQLAAAQPENH
  ASAFPRARCQGVSVLRKRYSEPVSLGSAQSPPLSPLALGIKEHELVTGLAQFNLQGSQGMEETSQLTLSD
  FSPMELESVGHRGPQSLSASSSSLTPMGRDALGSLSPALEDGKAAPDEPGSAALGSVGSSDPENKDPRPL
  FCISDTTDCSLTLDCSEGTDSRPRGGDPEEGGEGDGSMSSGVGEMGDSQVSSNPTSSPPGEAPAPVSVDS
  EPSCKGGLPRDKPTKRKDVVAPKRGSLKEASPGASKPGSARRSQGAVAKSVRTLTASENESMRKVMPITK
  SSRGAGWRRPELSSRGPSQNPPSSTDTVWSRQNSVRRASTGAEEQRLPRGSSGSSSTRPGRDVPLQPRGS
  FKKPSAKPLRNLPRQKPEENKTCRAHSEGPESPKEEPKTPSVPSVPHELPRVPSFARNTVASSSRSMRTD
  LPPVAKAPGITRTVSQRQLRVKGDPEDAAPKDSSTLRRASSARAPKKRPESAEGPSANTEAPLKARGAGE
  RASLRRKDSSRTTLGRILNPLRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033393
• ORF Size: 3429 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_033393.1, NM_033393.2, NP_203751.1, NP_203751.2
• RefSeq Size: 6470
• RefSeq ORF: 3432
• Locus ID: 85462
• MW: 124.6 kDa