DTX2 (NM_001102596) Human Tagged ORF Clone

CAT#: RC214817

DTX2 (Myc-DDK-tagged)-Human deltex homolog 2 (Drosophila) (DTX2), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY420165) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214817 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DTX2
• Synonyms: RNF58
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214817 representing NM_001102596
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCATGGCCCCAAGCCCTTCCCTGGTGCAGGTGTACACCAGCCCCGCGGCTGTGGCCGTGTGGGAAT
  GGCAGGACGGGCTGGGCACCTGGCACCCCTACAGTGCCACCGTCTGCAGCTTCATCGAGCAGCAGTTTGT
  CCAGCAGAAGGGCCAACGTTTTGGGCTTGGGAGCCTGGCCCACAGCATCCCCTTGGGCCAGGCAGACCCC
  TCGCTGGCCCCTTACATTATTGACCTCCCCAGCTGGACCCAGTTCCGCCAGGACACCGGCACCATGCGGG
  CTGTGCGGAGACACCTGTTCCCCCAGCACTCAGCCCCTGGCCGAGGTGTCGTCTGGGAGTGGCTGAGCGA
  CGATGGCTCCTGGACTGCCTATGAAGCCAGCGTCTGTGACTATCTGGAGCAGCAGGTGGCCAGGGGCAAC
  CAGCTCGTGGACTTGGCCCCCCTGGGGTACAACTACACTGTCAACTACACCACCCACACGCAGACCAACA
  AGACTTCCAGCTTCTGCCGCAGCGTGCGGCGCCAAGCAGGGCCGCCTTACCCGGTGACCACCATCATCGC
  TCCGCCGGGCCACACAGGCGTCGCCTGCTCTTGCCACCAGTGCCTCAGTGGCAGCAGAACTGGCCCCGTG
  TCAGGCCGCTACCGCCACTCCATGACCAACCTCCCTGCATACCCCGTCCCCCAGCACCCCCCACACAGGA
  CCGCTTCTGTGTTTGGGACCCACCAGGCCTTTGCACCGTACAACAAACCCTCACTCTCCGGGGCCCGGTC
  TGCGCCCAGGCTGAACACCACCAACGCCTGGGGCGCAGCTCCTCCTTCCCTGGGGAGCCAGCCCCTCTAC
  CGCTCCAGCCTCTCCCACCTGGGACCGCAGCACCTGCCCCCAGGATCCTCCACCTCCGGTGCAGTCAGTG
  CCTCCCTCCCCAGCGGTCCCTCAAGCAGCCCAGGGAGCGTCCCTGCCACTGTGCCCATGCAGATGCCAAA
  GCCCAGCAGAGTCCAGCAGGCGCTCGCAGGAGCGACCCCGAAGCCAGAGCCAGAGCCAGAGCAGGTCATA
  AAAAACTACACGGAAGAGCTGAAAGTGCCCCCAGATGAGGACTGCATCATCTGCATGGAGAAGCTGTCCA
  CAGCGTCTGGATACAGCGATGTGACTGACAGCAAGGCAATCGGGTCCCTAGCTGTGGGCCACCTCACCAA
  GTGCAGCCATGCCTTCCACCTGCTGTGCCTCCTGGCCATGTACTGCAACGGCAATAAGGATGGAAGTCTG
  CAGTGTCCCTCCTGCAAAACCATCTATGGAGAGAAGACGGGGACCCAGCCCCAGGGAAAGATGGAGGTAT
  TACGGTTCCAGATGTCGCTCCCCGGCCACGAGGACTGCGGGACCATCCTCATAGTTTACAGCATTCCCCA
  TGGTATCCAGGGCCCTGAGCACCCCAATCCCGGAAAGCCGTTCACTGCCAGAGGGTTTCCCCGCCAGTGC
  TACCTTCCAGACAACGCCCAGGGCCGCAAGGTCCTAGAGCTCCTGAAGGTGGCCTGGAAGAGGCGGCTCA
  TCTTCACAGTGGGCACGTCCAGCACCACGGGTGAGACGGACACCGTGGTATGGAACGAGATCCACCACAA
  GACAGAGATGGACCGCAACATTACGGGCCACGGCTATCCCGACCCCAACTACCTGCAGAACGTGCTGGCT
  GAGCTGGCTGCCCAGGGGGTGACCGAGGACTGCCTGGAGCAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214817 representing NM_001102596
  Red=Cloning site Green=Tags(s)
  
  MAMAPSPSLVQVYTSPAAVAVWEWQDGLGTWHPYSATVCSFIEQQFVQQKGQRFGLGSLAHSIPLGQADP
  SLAPYIIDLPSWTQFRQDTGTMRAVRRHLFPQHSAPGRGVVWEWLSDDGSWTAYEASVCDYLEQQVARGN
  QLVDLAPLGYNYTVNYTTHTQTNKTSSFCRSVRRQAGPPYPVTTIIAPPGHTGVACSCHQCLSGSRTGPV
  SGRYRHSMTNLPAYPVPQHPPHRTASVFGTHQAFAPYNKPSLSGARSAPRLNTTNAWGAAPPSLGSQPLY
  RSSLSHLGPQHLPPGSSTSGAVSASLPSGPSSSPGSVPATVPMQMPKPSRVQQALAGATPKPEPEPEQVI
  KNYTEELKVPPDEDCIICMEKLSTASGYSDVTDSKAIGSLAVGHLTKCSHAFHLLCLLAMYCNGNKDGSL
  QCPSCKTIYGEKTGTQPQGKMEVLRFQMSLPGHEDCGTILIVYSIPHGIQGPEHPNPGKPFTARGFPRQC
  YLPDNAQGRKVLELLKVAWKRRLIFTVGTSSTTGETDTVVWNEIHHKTEMDRNITGHGYPDPNYLQNVLA
  ELAAQGVTEDCLEQQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001102596
• ORF Size: 1725 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001102596.1, NP_001096066.1
• RefSeq Size: 2138
• RefSeq ORF: 1728
• Locus ID: 113878
• Protein Families: Druggable Genome
• Protein Pathways: Notch signaling pathway
• MW: 62.2 kDa
• Gene Summary: DTX2 functions as an E3 ubiquitin ligase (Takeyama et al., 2003 [PubMed 12670957]). [supplied by OMIM, Nov 2009]