Neuron navigator 1 (NAV1) (NM_020443) Human Tagged ORF Clone

CAT#: RC214825

NAV1 (Myc-DDK-tagged)-Human neuron navigator 1 (NAV1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412451) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214825 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAV1
• Synonyms: POMFIL3; STEERIN1; UNC53H1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214825 representing NM_020443
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGCAGCAGCGTCAAGAGCGTGCAGCCCGAGGTGGAGCTGAGCAGCGGCGGCGGCGACGAGGGCG
  CGGACGAACCGCGGGGCGCCGGCAGGAAGGCGGCAGCGGCGGACGGCAGAGGCATGCTGCCCAAGCGCGC
  CAAGGCGCCCGGCGGCGGCGGCGGCATGGCCAAGGCCAGCGCGGCTGAGCTGAAGGTCTTCAAGTCCGGC
  AGCGTGGACAGCCGTGTCCCCGGCGGGCCGCCCGCCTCCAACCTGCGCAAGCAGAAGTCACTCACCAACC
  TCTCTTTTCTCACGGACTCCGAGAAAAAGCTGCAGCTTTATGAGCCCGAATGGAGCGACGATATGGCCAA
  GGCGCCCAAAGGCTTAGGCAAGGTGGGGTCCAAGGGCCGTGAAGCTCCGCTGATGTCCAAGACGCTGTCC
  AAGTCGGAGCACTCGCTCTTCCAGGCCAAGGGCAGCCCGGCGGGCGGTGCCAAGACCCCCCTGGCTCCGC
  TCGCGCCCAACCTGGGAAAGCCGAGCCGGATCCCTCGAGGACCCTATGCGGAGGTCAAGCCGCTCAGCAA
  GGCGCCTGAAGCGGCCGTGAGCGAAGATGGCAAATCGGACGACGAGCTGCTCTCCAGCAAGGCCAAGGCG
  CAAAAGAGCTCTGGGCCTGTCCCCTCTGCCAAGGGCCAGGAGGAGCGCGCCTTCCTCAAGGTGGACCCCG
  AGCTGGTGGTGACCGTGCTGGGAGACCTGGAGCAGCTGCTCTTCAGCCAGATGCTGGACCCAGAGTCCCA
  GAGAAAGAGGACAGTGCAGAATGTCCTGGATCTCCGGCAGAACCTGGAAGAGACCATGTCCAGCCTGCGA
  GGGTCCCAGGTGACTCACAGCTCCCTGGAGATGACCTGCTACGACAGCGATGATGCCAACCCACGCAGCG
  TGTCCAGCCTCTCCAACCGCTCGTCCCCTCTGTCATGGCGCTATGGCCAGTCCAGTCCGCGGCTGCAGGC
  TGGTGACGCGCCCTCTGTGGGTGGGAGCTGCCGCTCGGAGGGGACGCCCGCCTGGTACATGCACGGCGAA
  CGGGCCCACTACTCCCACACCATGCCCATGCGCAGCCCCAGCAAGCTCAGCCATATCTCCCGCCTGGAGC
  TGGTCGAATCCCTGGACTCGGATGAGGTGGACCTCAAGTCCGGCTACATGAGCGACAGTGACCTCATGGG
  CAAGACCATGACGGAGGATGATGACATCACTACCGGCTGGGATGAAAGCAGCTCCATCAGTAGTGGACTC
  AGCGATGCCTCAGACAATCTCAGTTCAGAAGAATTCAATGCCAGCTCCTCACTCAACTCCCTCCCAAGTA
  CTCCCACTGCTTCTCGCAGGAACTCAACAATAGTGCTACGCACAGACTCAGAGAAGCGCTCACTGGCAGA
  AAGTGGGCTGAGCTGGTTTAGTGAATCAGAGGAGAAAGCCCCTAAAAAACTGGAGTACGACAGTGGTAGC
  CTGAAGATGGAACCTGGGACTTCTAAGTGGCGGAGGGAGCGGCCTGAGAGCTGTGATGATTCATCCAAGG
  GTGGAGAACTGAAAAAGCCCATCAGCCTGGGCCACCCTGGTTCCCTGAAGAAGGGCAAGACCCCACCTGT
  GGCTGTAACTTCCCCCATCACTCACACAGCCCAGAGTGCCCTCAAAGTCGCAGGCAAACCTGAGGGCAAA
  GCTACAGACAAGGGTAAGCTTGCAGTGAAGAATACTGGGCTCCAACGCTCCTCCTCTGATGCTGGTCGGG
  ACCGCCTGAGTGATGCTAAGAAGCCCCCCTCGGGCATTGCTCGCCCCTCCACTTCGGGATCCTTTGGCTA
  CAAGAAGCCTCCTCCTGCCACAGGCACAGCCACTGTCATGCAAACTGGTGGTTCAGCCACTCTCAGCAAG
  ATCCAGAAGTCCTCAGGCATCCCTGTCAAGCCAGTAAATGGGCGCAAGACTAGCTTAGATGTTTCCAACA
  GTGCAGAGCCAGGATTCCTGGCTCCTGGAGCCCGTTCTAACATCCAGTACCGCAGCCTGCCCCGGCCAGC
  CAAGTCAAGTTCTATGAGCGTGACCGGCGGGCGGGGTGGACCTCGCCCTGTGAGCAGCAGCATTGACCCC
  AGTCTCCTCAGCACCAAGCAGGGAGGCCTTACGCCTTCCAGACTGAAGGAGCCTACCAAGGTAGCCAGTG
  GGCGGACCACTCCAGCCCCTGTCAATCAGACAGATCGGGAAAAGGAGAAGGCCAAAGCCAAGGCAGTGGC
  CTTGGACTCAGACAACATCTCCTTGAAGAGTATTGGCTCCCCAGAAAGTACTCCCAAGAACCAAGCAAGC
  CACCCCACAGCCACCAAGCTGGCAGAGCTGCCACCAACCCCTCTCAGGGCCACAGCGAAGAGCTTTGTCA
  AACCACCCTCACTAGCCAATCTTGACAAGGTCAACTCCAACAGTCTGGATCTACCATCATCCAGTGATAC
  CACCCATGCTTCAAAGGTCCCAGATCTGCATGCTACAAGCTCAGCATCTGGGGGCCCTCTCCCTTCCTGC
  TTCACCCCCAGTCCGGCACCCATCCTCAATATTAACTCAGCCAGCTTCTCCCAGGGCCTGGAGCTAATGA
  GTGGTTTCAGTGTGCCAAAAGAGACCCGCATGTACCCCAAACTCTCAGGCCTGCACAGGAGCATGGAGTC
  CCTCCAGATGCCAATGAGCCTCCCCAGTGCCTTCCCCAGCAGTACTCCCGTCCCCACCCCACCTGCTCCC
  CCTGCTGCTCCCACAGAAGAAGAGACGGAAGAGCTGACTTGGAGTGGAAGCCCCAGAGCTGGGCAACTGG
  ACAGTAATCAGCGGGATCGGAACACTCTTCCCAAGAAAGGGCTCAGGTACCAGCTTCAGTCCCAGGAGGA
  GACCAAGGAGAGGCGACATTCCCATACCATTGGTGGGCTGCCTGAATCCGATGACCAGTCAGAGCTGCCT
  TCTCCCCCTGCACTTCCCATGTCTCTGAGTGCAAAGGGCCAACTTACCAACATAGTGAGTCCCACTGCGG
  CCACCACGCCAAGAATCACCCGCTCCAACAGCATCCCCACCCACGAGGCGGCCTTCGAGCTGTACAGCGG
  CTCCCAAATGGGGAGCACCCTGTCCCTGGCCGAGAGACCCAAGGGAATGATTCGGTCAGGATCCTTCCGA
  GACCCCACGGACGATGTTCACGGCTCAGTGCTGTCCCTGGCCTCCAGTGCCTCCTCCACCTACTCCTCAG
  CTGAGGAGAGGATGCAATCTGAGCAAATCCGGAAGCTTCGTAGGGAACTGGAATCATCCCAGGAAAAAGT
  GGCCACCTTGACGTCTCAGCTTTCTGCCAATGCTAATCTGGTGGCTGCTTTTGAGCAGAGCCTGGTGAAT
  ATGACATCCCGCCTGCGACACCTGGCAGAGACGGCCGAGGAGAAGGACACTGAGCTGCTGGATTTGCGAG
  AAACCATAGACTTTCTGAAGAAAAAGAACTCTGAGGCCCAGGCAGTCATTCAGGGAGCCCTTAATGCCTC
  AGAAACCACACCCAAAGAACTTCGGATCAAGAGACAAAACTCCTCAGATAGCATCTCAAGCCTCAACAGC
  ATCACTAGCCATTCCAGCATCGGCAGCAGCAAGGATGCTGATGCGAAAAAGAAGAAAAAAAAGAGTTGGC
  TTCGAAGTTCCTTCAACAAAGCGTTCAGTATAAAAAAGGGGCCCAAGTCAGCTTCCTCATACTCGGATAT
  AGAGGAGATTGCTACACCCGACTCTTCAGCCCCCTCATCCCCCAAACTACAGCATGGTTCTACAGAGACT
  GCTTCACCCTCCATCAAGTCCTCCACCTCGTCCTCCGTGGGCACTGATGTCACCGAGGGCCCTGCTCACC
  CAGCCCCCCACACTAGGCTGTTCCATGCAAATGAGGAGGAGGAGCCAGAGAAGAAGGAGGTATCGGAGCT
  GCGCTCTGAGCTATGGGAGAAGGAAATGAAGCTTACAGACATCCGCTTGGAGGCCCTCAACTCTGCCCAC
  CAACTGGATCAGCTTCGGGAGACCATGCACAACATGCAGTTGGAGGTGGACCTGCTGAAAGCAGAGAATG
  ACCGACTGAAGGTAGCCCCAGGCCCCTCATCAGGCTCCACTCCAGGGCAGGTCCCTGGATCATCTGCATT
  ATCTTCCCCACGCCGCTCCCTAGGCCTGGCACTCACCCATTCCTTCGGCCCCAGTCTTGCAGACACAGAC
  CTGTCACCCATGGATGGCATCAGTACTTGTGGTCCAAAGGAGGAAGTGACCCTCCGGGTGGTGGTGAGGA
  TGCCCCCGCAGCACATCATCAAAGGGGACTTGAAGCAGCAGGAATTCTTCCTGGGCTGTAGCAAGGTCAG
  TGGAAAAGTTGACTGGAAGATGCTGGATGAAGCTGTTTTCCAAGTGTTCAAGGACTATATTTCTAAAATG
  GACCCAGCCTCTACCCTGGGACTAAGCACTGAGTCCATCCATGGCTACAGCATCAGCCACGTGAAACGAG
  TGTTGGATGCAGAGCCCCCCGAGATGCCTCCTTGCCGTCGAGGTGTCAATAACATATCAGTCTCCCTCAA
  AGGTCTGAAGGAGAAATGCGTCGACAGCCTGGTGTTCGAGACGCTGATCCCCAAGCCGATGATGCAGCAC
  TACATAAGCCTCCTGCTGAAGCACCGGCGCCTCGTCCTCTCGGGCCCCAGCGGCACGGGCAAGACCTACC
  TGACCAATCGCTTGGCCGAGTACCTGGTGGAGCGCTCTGGCCGTGAGGTCACAGAGGGCATCGTCAGCAC
  CTTCAACATGCACCAGCAGTCTTGCAAGGATCTGCAACTGTATCTTTCCAACCTAGCCAACCAGATAGAC
  CGGGAAACAGGAATTGGGGATGTGCCCCTGGTGATTCTATTGGATGACCTGAGTGAAGCAGGCTCCATCA
  GTGAGTTGGTCAATGGGGCCCTCACCTGCAAGTATCATAAATGTCCCTATATTATAGGTACCACCAATCA
  GCCTGTAAAAATGACACCCAACCATGGCTTGCACTTGAGCTTCAGGATGTTGACCTTCTCCAACAACGTG
  GAGCCAGCCAATGGCTTCCTGGTTCGTTACCTGAGGAGGAAGCTGGTAGAGTCAGACAGCGACATCAATG
  CCAACAAGGAAGAGCTGCTTCGGGTGCTCGACTGGGTACCCAAGCTGTGGTATCATCTCCACACCTTCCT
  TGAGAAGCACAGCACCTCAGACTTCCTCATCGGCCCTTGCTTCTTTCTGTCGTGTCCCATTGGCATTGAG
  GACTTCCGGACCTGGTTCATTGACCTGTGGAACAACTCTATCATTCCCTATCTACAGGAAGGAGCCAAGG
  ATGGGATAAAGGTCCATGGACAGAAAGCTGCTTGGGAGGACCCAGTGGAATGGGTCCGGGACACACTTCC
  CTGGCCATCAGCCCAACAAGACCAATCAAAGCTGTACCACCTGCCCCCACCCACCGTGGGCCCTCACAGC
  ATTGCCTCACCTCCCGAGGATAGGACAGTCAAAGACAGCACCCCAAGTTCTCTGGACTCAGATCCTCTGA
  TGGCCATGCTGCTGAAACTTCAAGAAGCTGCCAACTACATTGAGTCTCCAGATCGAGAAACCATCCTGGA
  CCCCAACCTTCAGGCAACACTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214825 representing NM_020443
  Red=Cloning site Green=Tags(s)
  
  MLGSSVKSVQPEVELSSGGGDEGADEPRGAGRKAAAADGRGMLPKRAKAPGGGGGMAKASAAELKVFKSG
  SVDSRVPGGPPASNLRKQKSLTNLSFLTDSEKKLQLYEPEWSDDMAKAPKGLGKVGSKGREAPLMSKTLS
  KSEHSLFQAKGSPAGGAKTPLAPLAPNLGKPSRIPRGPYAEVKPLSKAPEAAVSEDGKSDDELLSSKAKA
  QKSSGPVPSAKGQEERAFLKVDPELVVTVLGDLEQLLFSQMLDPESQRKRTVQNVLDLRQNLEETMSSLR
  GSQVTHSSLEMTCYDSDDANPRSVSSLSNRSSPLSWRYGQSSPRLQAGDAPSVGGSCRSEGTPAWYMHGE
  RAHYSHTMPMRSPSKLSHISRLELVESLDSDEVDLKSGYMSDSDLMGKTMTEDDDITTGWDESSSISSGL
  SDASDNLSSEEFNASSSLNSLPSTPTASRRNSTIVLRTDSEKRSLAESGLSWFSESEEKAPKKLEYDSGS
  LKMEPGTSKWRRERPESCDDSSKGGELKKPISLGHPGSLKKGKTPPVAVTSPITHTAQSALKVAGKPEGK
  ATDKGKLAVKNTGLQRSSSDAGRDRLSDAKKPPSGIARPSTSGSFGYKKPPPATGTATVMQTGGSATLSK
  IQKSSGIPVKPVNGRKTSLDVSNSAEPGFLAPGARSNIQYRSLPRPAKSSSMSVTGGRGGPRPVSSSIDP
  SLLSTKQGGLTPSRLKEPTKVASGRTTPAPVNQTDREKEKAKAKAVALDSDNISLKSIGSPESTPKNQAS
  HPTATKLAELPPTPLRATAKSFVKPPSLANLDKVNSNSLDLPSSSDTTHASKVPDLHATSSASGGPLPSC
  FTPSPAPILNINSASFSQGLELMSGFSVPKETRMYPKLSGLHRSMESLQMPMSLPSAFPSSTPVPTPPAP
  PAAPTEEETEELTWSGSPRAGQLDSNQRDRNTLPKKGLRYQLQSQEETKERRHSHTIGGLPESDDQSELP
  SPPALPMSLSAKGQLTNIVSPTAATTPRITRSNSIPTHEAAFELYSGSQMGSTLSLAERPKGMIRSGSFR
  DPTDDVHGSVLSLASSASSTYSSAEERMQSEQIRKLRRELESSQEKVATLTSQLSANANLVAAFEQSLVN
  MTSRLRHLAETAEEKDTELLDLRETIDFLKKKNSEAQAVIQGALNASETTPKELRIKRQNSSDSISSLNS
  ITSHSSIGSSKDADAKKKKKKSWLRSSFNKAFSIKKGPKSASSYSDIEEIATPDSSAPSSPKLQHGSTET
  ASPSIKSSTSSSVGTDVTEGPAHPAPHTRLFHANEEEEPEKKEVSELRSELWEKEMKLTDIRLEALNSAH
  QLDQLRETMHNMQLEVDLLKAENDRLKVAPGPSSGSTPGQVPGSSALSSPRRSLGLALTHSFGPSLADTD
  LSPMDGISTCGPKEEVTLRVVVRMPPQHIIKGDLKQQEFFLGCSKVSGKVDWKMLDEAVFQVFKDYISKM
  DPASTLGLSTESIHGYSISHVKRVLDAEPPEMPPCRRGVNNISVSLKGLKEKCVDSLVFETLIPKPMMQH
  YISLLLKHRRLVLSGPSGTGKTYLTNRLAEYLVERSGREVTEGIVSTFNMHQQSCKDLQLYLSNLANQID
  RETGIGDVPLVILLDDLSEAGSISELVNGALTCKYHKCPYIIGTTNQPVKMTPNHGLHLSFRMLTFSNNV
  EPANGFLVRYLRRKLVESDSDINANKEELLRVLDWVPKLWYHLHTFLEKHSTSDFLIGPCFFLSCPIGIE
  DFRTWFIDLWNNSIIPYLQEGAKDGIKVHGQKAAWEDPVEWVRDTLPWPSAQQDQSKLYHLPPPTVGPHS
  IASPPEDRTVKDSTPSSLDSDPLMAMLLKLQEAANYIESPDRETILDPNLQATL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020443
• ORF Size: 5622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020443.2, NM_020443.3, NM_020443.4, NP_065176.2, NP_065176.3
• RefSeq Size: 11365
• RefSeq ORF: 5634
• Locus ID: 89796
• Domains: AAA
• MW: 202.3 kDa
• Gene Summary: This gene belongs to the neuron navigator family and is expressed predominantly in the nervous system. The encoded protein contains coiled-coil domains and a conserved AAA domain characteristic for ATPases associated with a variety of cellular activities. This gene is similar to unc-53, a Caenorhabditis elegans gene involved in axon guidance. The exact function of this gene is not known, but it is thought to play a role in in neuronal development and regeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2009]