MINA53 (MINA) (NM_001042533) Human Tagged ORF Clone

CAT#: RC214829

MINA (Myc-DDK-tagged)-Human MYC induced nuclear antigen (MINA), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420967) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214829 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIOX2
• Synonyms: JMJD10; MDIG; MINA; MINA53; NO52; ROX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214829 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAAAGAAAGCAAAGCCTACAGGGAGTGGGAAGGAAGAGGGGCCGGCTCCCTGTAAGCAGATGAAGT
  TAGAAGCAGCTGGGGGGCCTTCAGCTTTAAACTTTGACAGTCCCAGTAGTCTCTTTGAAAGTTTAATCTC
  GCCCATCAAGACAGAGACTTTTTTCAAGGAATTCTGGGAGCAGAAGCCCCTTCTCATTCAGAGAGATGAC
  CCTGCACTGGCCACATACTATGGGTCCCTGTTCAAGCTAACAGATCTGAAGAGTCTGTGCAGCCGGGGGA
  TGTACTATGGAAGAGATGTGAATGTCTGCCGGTGTGTCAATGGGAAGAAGAAGGTTTTAAATAAAGATGG
  CAAAGCACACTTTCTTCAGCTGAGAAAAGATTTTGATCAGAAAAGGGCAACGATTCAGTTTCACCAACCT
  CAGAGATTTAAGGATGAGCTTTGGAGGATCCAGGAGAAGCTGGAATGTTACTTTGGCTCCTTGGTTGGCT
  CGAATGTGTACATAACTCCCGCAGGATCTCAGGGCCTGCCGCCCCATTATGATGATGTCGAGGTTTTCAT
  CCTGCAGCTGGAGGGAGAGAAACACTGGCGCCTCTACCACCCCACTGTGCCCCTGGCACGAGAGTACAGC
  GTGGAGGCCGAGGAAAGGATCGGCAGGCCGGTGCATGAGTTTATGCTGAAGCCGGGTGATTTGTTGTACT
  TTCCCAGAGGAACCATTCATCAAGCGGACACTCCTGCGGGGCTGGCCCACTCGACTCACGTGACCATCAG
  CACCTACCAGAACAATTCATGGGGAGATTTCCTTTTGGATACCATCTCGGGGCTTGTATTTGATACTGCA
  AAGGAAGACGTGGAGTTACGGACCGGCATACCCCGGCAGCTGCTCCTGCAGGTGGAATCCACAACTGTTG
  CTACAAGACGATTAAGTGGCTTCCTGAGGACACTTGCAGACCGGCTGGAGGGCACCAAAGAACTGCTTTC
  CTCAGACATGAAGAAGGATTTTATTATGCACAGACTCCCCCCTTACTCTGCGGGAGATGGGGCAGAGCTG
  TCAACACCAGGTGGAAAGTTACCGAGGCTGGACAGTGTAGTGAGACTGCAGTTTAAAGACCACATTGTCC
  TCACAGTACTGCCGGATCAAGATCAATCTGATGAAACTCAAGAAAAGATGGTGTACATCTATCATTCCTT
  AAAGAATAGTAGAGAGACACACATGATGGGAAATGAGGAGGAAACAGAGTTTCATGGACTTCGCTTCCCT
  TTGTCACATTTGGATGCACTGAAGCAAATTTGGAATAGTCCAGCTATTTCTGTCAAGGACCTGAAACTTA
  CTACAGATGAGGAAAAGGAAAGCCTGGTATTATCCCTCTGGACAGAATGTTTAATTCAAGTAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214829 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPKKAKPTGSGKEEGPAPCKQMKLEAAGGPSALNFDSPSSLFESLISPIKTETFFKEFWEQKPLLIQRDD
  PALATYYGSLFKLTDLKSLCSRGMYYGRDVNVCRCVNGKKKVLNKDGKAHFLQLRKDFDQKRATIQFHQP
  QRFKDELWRIQEKLECYFGSLVGSNVYITPAGSQGLPPHYDDVEVFILQLEGEKHWRLYHPTVPLAREYS
  VEAEERIGRPVHEFMLKPGDLLYFPRGTIHQADTPAGLAHSTHVTISTYQNNSWGDFLLDTISGLVFDTA
  KEDVELRTGIPRQLLLQVESTTVATRRLSGFLRTLADRLEGTKELLSSDMKKDFIMHRLPPYSAGDGAEL
  STPGGKLPRLDSVVRLQFKDHIVLTVLPDQDQSDETQEKMVYIYHSLKNSRETHMMGNEEETEFHGLRFP
  LSHLDALKQIWNSPAISVKDLKLTTDEEKESLVLSLWTECLIQVV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042533
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042533.1, NM_001042533.2, NP_001035998.1
• RefSeq Size: 5342 bp
• RefSeq ORF: 1398 bp
• Locus ID: 84864
• Protein Families: Druggable Genome
• MW: 52.8 kDa
• Gene Summary: MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]). [supplied by OMIM, May 2008]