SLC13A2 (NM_003984) Human Tagged ORF Clone

CAT#: RC214998

SLC13A2 (Myc-DDK-tagged)-Human solute carrier family 13 (sodium-dependent dicarboxylate transporter), member 2 (SLC13A2), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY418310) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC214998 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC13A2
• Synonyms: NaCT; NaDC-1; NADC1; SDCT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC214998 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACCTGCTGGCAGGCCCTGTGGGCCTATCGCTCCTACCTGATCGTGTTCTTCGTGCCCATTCTCC
  TGCTGCCTCTGCCCATCCTCGTCCCCAGTAAGGAGGCCTACTGCGCGTATGCCATCATCCTCATGGCGCT
  CTTCTGGTGCACTGAGGCCCTGCCCCTGGCCGTCACTGCCCTCTTCCCCTTAATCCTGTTCCCTATGATG
  GGCATCGTGGATGCCTCTGAGGTTGCCGTCGAGTATCTTAAGGACTCCAACCTCCTGTTCTTCGGGGGGC
  TGCTGGTGGCCATCGCGGTGGAACACTGGAACCTGCATAAACGCATCGCCCTCCGTGTCCTCCTCATCGT
  TGGGGTGCGGCCTGCCCCGCTAATCCTGGGCTTCATGCTGGTCACGGCCTTCCTGTCCATGTGGATCAGC
  AACACGGCCACCTCAGCCATGATGGTGCCCATCGCACATGCCGTCCTGGACCAGCTGCACAGCTCGCAAG
  CCAGCAGCAACGTCGAGGAGGGCAGCAACAACCCCACCTTCGAGCTCCAGGAACCAAGTCCCCAGAAGGA
  GGTGACCAAGCTTGATAATGGGCAGGCCCTCCCTGTCACGTCTGCCTCTTCGGAGGGGAGGGCACATCTC
  AGCCAGAAGCATCTCCACCTCACCCAGTGCATGAGCCTGTGCGTGTGCTACTCCGCCAGCATCGGGGGCA
  TCGCCACGCTGACTGGCACCGCACCCAACCTGGTGCTGCAAGGCCAGATCAACTCGCTCTTCCCCCAAAA
  CGGCAACGTGGTGAACTTCGCCTCCTGGTTCAGCTTCGCCTTCCCCACCATGGTCATCTTGCTGCTGCTG
  GCCTGGTTGTGGCTGCAGATCCTCTTCCTGGGCTTCAACTTCCGGAAGAACTTTGGCATTGGGGAAAAGA
  TGCAGGAGCAACAGCAGGCAGCCTACTGCGTCATCCAGACCGAGCACAGGCTGCTGGGCCCCATGACCTT
  TGCAGAAAAGGCCATCAGCATCCTATTCGTCATCCTGGTGCTGCTCTGGTTCACCCGGGAGCCGGGCTTT
  TTTCTTGGCTGGGGCAATTTGGCTTTTCCCAATGCCAAGGGGGAGAGCATGGTGTCCGATGGGACAGTGG
  CCATCTTCATCGGCATAATTATGTTCATCATACCCTCCAAGTTCCCAGGGCTGACCCAGGACCCAGAAAA
  CCCAGGGAAGCTGAAGGCCCCTCTTGGCCTCCTCGACTGGAAGACGGTGAACCAGAAGATGCCGTGGAAT
  ATCGTGTTATTGCTGGGTGGTGGCTATGCCCTGGCCAAGGGCAGTGAGCGATCGGGCCTGTCAGAGTGGC
  TGGGAAACAAGCTGACCCCACTGCAGAGTGTGCCAGCTCCAGCCATTGCCATCATCCTCTCCCTCCTGGT
  GGCCACCTTCACCGAGTGCACTAGCAACGTGGCCACCACTACGATCTTCCTGCCCATCCTAGCCTCCATG
  GCCCAGGCCATCTGCCTCCACCCTCTCTACGTCATGCTCCCCTGCACTCTGGCCACCTCCCTGGCCTTCA
  TGTTGCCTGTGGCCACCCCGCCCAATGCCATCGTCTTCTCTTTCGGGGACCTCAAAGTGTTGGATATGGC
  CCGGGCAGGATTCCTCCTCAACATCATTGGAGTCCTGATCATCGCACTGGCCATCAACAGCTGGGGCATC
  CCCCTCTTCAGCCTGCACTCTTTCCCCTCCTGGGCACAGTCCAACACCACAGCCCAGTGCCTGCCAAGCC
  TGGCCAACACCACCACACCAAGCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC214998 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATCWQALWAYRSYLIVFFVPILLLPLPILVPSKEAYCAYAIILMALFWCTEALPLAVTALFPLILFPMM
  GIVDASEVAVEYLKDSNLLFFGGLLVAIAVEHWNLHKRIALRVLLIVGVRPAPLILGFMLVTAFLSMWIS
  NTATSAMMVPIAHAVLDQLHSSQASSNVEEGSNNPTFELQEPSPQKEVTKLDNGQALPVTSASSEGRAHL
  SQKHLHLTQCMSLCVCYSASIGGIATLTGTAPNLVLQGQINSLFPQNGNVVNFASWFSFAFPTMVILLLL
  AWLWLQILFLGFNFRKNFGIGEKMQEQQQAAYCVIQTEHRLLGPMTFAEKAISILFVILVLLWFTREPGF
  FLGWGNLAFPNAKGESMVSDGTVAIFIGIIMFIIPSKFPGLTQDPENPGKLKAPLGLLDWKTVNQKMPWN
  IVLLLGGGYALAKGSERSGLSEWLGNKLTPLQSVPAPAIAIILSLLVATFTECTSNVATTTIFLPILASM
  AQAICLHPLYVMLPCTLATSLAFMLPVATPPNAIVFSFGDLKVLDMARAGFLLNIIGVLIIALAINSWGI
  PLFSLHSFPSWAQSNTTAQCLPSLANTTTPSP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003984
• ORF Size: 1776 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003984.1, NM_003984.2, NM_003984.3, NP_003975.1
• RefSeq Size: 2384 bp
• RefSeq ORF: 1779 bp
• Locus ID: 9058
• Protein Families: Transmembrane
• MW: 64.4 kDa
• Gene Summary: The protein encoded by this gene is a sodium-coupled citrate transporter that is regulated by the chaperone activity of cyclophilin b. The encoded protein may play a role in the formation of kidney stones. [provided by RefSeq, Oct 2016]