FGR (NM_001042747) Human Tagged ORF Clone

CAT#: RC215016

FGR (Myc-DDK-tagged)-Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY420802) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215016 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FGR
• Synonyms: c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; SRC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215016 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGCCTGGAAGGGG
  ACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAGGCCCGGCCTGCATCCTCATT
  TGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCCATCAACCCTGGCTTCCTTGATAGTGGCACC
  ATCAGGGGTGTGTCAGGGATTGGGGTGACCCTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGG
  ATGACCTCACCTTCACCAAGGGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGC
  TCGGTCTCTCAGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAA
  GCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCACCAGGCAACC
  CCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTACTCCCTGTCCATCCGGGACTG
  GGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATCCGCAAACTGGACATGGGCGGCTACTACATC
  ACCACACGGGTTCAGTTCAACTCGGTGCAGGAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGT
  GCAACCTGCTCATCGCGCCCTGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGA
  GATCAGCCGCAGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGC
  ACGTGGAACGGCAGCACTAAGGTGGCGGTGAAGACGCTGAAGCCGGGCACCATGTCCCCGAAGGCCTTCC
  TGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAGCTGTACGCCGTGGTGTCGGA
  GGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGCAGCTTGCTGGATTTTCTCAAGAACCCAGAG
  GGCCAGGATTTGAGGCTGCCCCAATTGGTGGACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGG
  AACGCATGAACTACATTCACCGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAA
  GATCGCAGACTTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTC
  CCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGACGTGTGGTCCT
  TTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCAGGCATGAATAAACGGGAAGT
  GTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCTCCAGGCTGCCCAGCATCCCTGTACGAGGCC
  ATGGAACAGACCTGGCGTCTGGACCCGGAGGAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGG
  ACTACTTCACCTCCGCTGAACCACAGTACCAGCCCGGGGATCAGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215016 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGCVFCKKLEPVATAKEDAGLEGDFRSYGAADHYGPDPTKARPASSFAHIPNYSNFSSQAINPGFLDSGT
  IRGVSGIGVTLFIALYDYEARTEDDLTFTKGEKFHILNNTEGDWWEARSLSSGKTGCIPSNYVAPVDSIQ
  AEEWYFGKIGRKDAERQLLSPGNPQGAFLIRESETTKGAYSLSIRDWDQTRGDHVKHYKIRKLDMGGYYI
  TTRVQFNSVQELVQHYMEVNDGLCNLLIAPCTIMKPQTLGLAKDAWEISRSSITLERRLGTGCFGDVWLG
  TWNGSTKVAVKTLKPGTMSPKAFLEEAQVMKLLRHDKLVQLYAVVSEEPIYIVTEFMCHGSLLDFLKNPE
  GQDLRLPQLVDMAAQVAEGMAYMERMNYIHRDLRAANILVGERLACKIADFGLARLIKDDEYNPCQGSKF
  PIKWTAPEAALFGRFTIKSDVWSFGILLTELITKGRIPYPGMNKREVLEQVEQGYHMPCPPGCPASLYEA
  MEQTWRLDPEERPTFEYLQSFLEDYFTSAEPQYQPGDQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042747
• ORF Size: 1587 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042747.1, NP_001036212.1
• RefSeq Size: 2501 bp
• RefSeq ORF: 1590 bp
• Locus ID: 2268
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway
• MW: 59.5 kDa
• Gene Summary: 'This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]'