GSG1 (NM_153823) Human Tagged ORF Clone

CAT#: RC215105

GSG1 (Myc-DDK-tagged)-Human germ cell associated 1 (GSG1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406967) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215105 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GSG1
• Synonyms: MGC3146; MGC111023
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215105 representing NM_153823
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAAGATGGAGCTCTCGAAGGCCTTCTCTGGCCAGCGGACACTCCTATCTGCCATCCTCAGCATGC
  TATCACTCAGCTTCTCCACAACATCCCTGCTCAGCAACTACTGGTTTGTGGGCACACAGAAGGTGCCCAA
  GCCCCTGTGCGAGAAAGGTCTGGCAGCCAAGTGCTTTGACATGCCAGTGTCCCTGGATGGAGATACCAAC
  ACATCCACCCAGGAGGTGGTACAATACAACTGGGAGACTGGGGATGACCGGTTCTCCTTCCGGAGCTTCC
  GGAGTGGCATGTGGCTATCCTGTGAGGAAACTGTGGAAGAACCAGGGGAGAGGTGCCGAAGTTTCATTGA
  ACTTACACCACCAGCCAAGAGAGAAATCCTATGGTTATCCCTGGGAACGCAGATCACCTACATCGGACTT
  CAATTCATCAGCTTCCTCCTGCTACTAACAGACTTGCTACTCACTGGGAACCCTGCCTGTGGGCTCAAAC
  TGAGCGCCTTTGCTGCTGTTTCCTCTGTCCTGTCAGGTCTCCTGGGGATGGTGGCCCACATGATGTATTC
  ACAAGTCTTCCAAGCGACTGTCAACTTGGGTCCAGAAGACTGGAGACCACATGTTTGGAATTATGGCTGG
  GCCTTCTACATGGCCTGGCTCTCCTTCACCTGCTGCATGGCGTCGGCTGTCACCACCTTCAACACGTACA
  CCAGGATGGTGCTGGAGTTCAAGTGCAAGCATAGTAAGAGCTTCAAGGAAAACCCGAACTGCCTACCACA
  TCACCATCAGTGTTTCCCTCGGCGGCTGTCAAGTGCAGCCCCCACCGTGGGTCCTTTGACCAGCTACCAC
  CAGTATCATAATCAGCCCATCCACTCTGTCTCTGAGGGAGTCGACTTCTACTCCGAGCTGCGGAACAAGG
  GATTTCAAAGAGGGGCCAGCCAGGAGCTGAAAGAAGCAGTTAGGTCATCTGTAGAGGAAGAGCAGTGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215105 representing NM_153823
  Red=Cloning site Green=Tags(s)
  
  MAKMELSKAFSGQRTLLSAILSMLSLSFSTTSLLSNYWFVGTQKVPKPLCEKGLAAKCFDMPVSLDGDTN
  TSTQEVVQYNWETGDDRFSFRSFRSGMWLSCEETVEEPGERCRSFIELTPPAKREILWLSLGTQITYIGL
  QFISFLLLLTDLLLTGNPACGLKLSAFAAVSSVLSGLLGMVAHMMYSQVFQATVNLGPEDWRPHVWNYGW
  AFYMAWLSFTCCMASAVTTFNTYTRMVLEFKCKHSKSFKENPNCLPHHHQCFPRRLSSAAPTVGPLTSYH
  QYHNQPIHSVSEGVDFYSELRNKGFQRGASQELKEAVRSSVEEEQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153823
• ORF Size: 978 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_153823.1, NM_153823.2, NM_153823.3, NP_722545.1, NP_722545.2
• RefSeq Size: 2391
• RefSeq ORF: 981
• Locus ID: 83445
• Protein Families: Transmembrane
• MW: 36.6 kDa
• Gene Summary: May cause the redistribution of PAPOLB from the cytosol to the endoplasmic reticulum. [UniProtKB/Swiss-Prot Function]