UBE3C (NM_014671) Human Tagged ORF Clone

CAT#: RC215110

UBE3C (Myc-DDK-tagged)-Human ubiquitin protein ligase E3C (UBE3C)

Product Images

Western blot validation of overexpression lysate (Cat# LY415125) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215110 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UBE3C protein (Cat# TP315110). The protein was produced from HEK293T cells transfected with UBE3C cDNA clone (Cat# RC215110) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UBE3C
• Synonyms: HECTH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215110 representing NM_014671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAGCTTCGAAGGCGACTTCAAGACGCGGCCCAAGGTGTCCCTTGGCGGCGCGAGCAGGAAGGAGG
  AAAAGGCTTCTCTTTTACATCGTACTCAGGAAGAAAGAAGAAAGAGAGAGGAAGAAAGGCGAAGGTTGAA
  AAATGCAATAATTATCCAGTCATTTATTCGAGGCTATAGAGACAGAAAACAGCAATATTCCATCCAAAGA
  AGTGCATTTGATCGCTGTGCTACCTTGTCACAGTCCGGGGGCGCTTTTCCCATTGCTAATGGCCCCAACC
  TTACCCTTTTGGTAAGGCAGCTTCTGTTTTTTTACAAACAAAATGAAGACTCAAAACGTTTGATATGGCT
  GTATCAGAACTTAATTAAACACAGCTCTCTGTTTGTCAAGCAGTTGGATGGATCTGAGAGACTTACATGC
  TTATTTCAGATAAAAAGATTGATGAGCCTCTGTTGCAGGTTGCTGCAAAACTGTAATGATGACAGTTTGA
  ATGTTGCACTTCCAATGAGAATGCTTGAAGTATTTTCGTCTGAGAATACTTACTTGCCTGTTTTACAAGA
  TGCTAGCTATGTGGTGTCAGTGATTGAACAAATTTTGCACTACATGATTCACAATGGGTATTATAGGTCT
  CTATATTTGTTGATTAACAGCAAGCTTCCATCAAGTATTGAATATTCTGATTTATCTCGAGTTCCTATAG
  CAAAAATTTTGCTAGAGAATGTTCTAAAACCATTGCACTTTACTTACAACTCCTGTCCGGAAGGTGCGAG
  GCAACAAGTTTTTACAGCCTTCACAGAGGAGTTTCTGGCAGCACCTTTTACAGATCAGATTTTTCATTTC
  ATCATTCCGGCGCTTGCAGATGCGCAGACCGTTTTCCCTTACGAGCCCTTTCTGAATGCACTGTTGTTAA
  TAGAGAGTAGATGTTCAAGAAAGAGTGGTGGAGCACCCTGGCTTTTCTATTTCGTTTTAACTGTTGGCGA
  AAATTATTTGGGGGCCCTCTCTGAGGAAGGGCTGCTGGTGTATTTGCGGGTGCTGCAGACCTTCCTCTCT
  CAGTTACCAGTCTCTCCTGCCAGCGCGAGCTGTCACGACTCAGCCAGTGACTCTGAGGAGGAGAGTGAAG
  AAGCCGACAAGCCCTCAAGCCCGGAGGATGGCAGACTGTCAGTATCATACATAACAGAGGAATGCCTGAA
  GAAGCTGGACACAAAGCAGCAGACCAACACCCTGCTCAACCTGGTGTGGAGGGACTCTGCGAGCGAGGAG
  GTCTTCACCACCATGGCCTCCGTCTGCCACACGCTGATGGTGCAGCACCGCATGATGGTACCCAAAGTCA
  GGCTTCTCTACAGTTTAGCCTTTAATGCCAGGTTTCTGAGACATCTTTGGTTTCTAATATCTTCCATGTC
  AACACGGATGATCACAGGGTCTATGGTACCGTTGCTTCAGGTGATATCCAGGGGTTCTCCTATGTCTTTT
  GAAGATTCTAGTCGAATCATCCCACTCTTTTATCTTTTTAGCTCCTTGTTTAGTCATTCACTAATTTCCA
  TACATGATAACGAATTCTTCGGTGATCCCATAGAAGTTGTAGGTCAAAGACAATCATCAATGATGCCTTT
  TACTTTAGAAGAGCTGATAATGTTGTCTCGATGCCTTCGAGATGCATGCCTGGGGATCATCAAGTTGGCT
  TATCCAGAAACCAAGCCAGAAGTTCGAGAAGAATATATTACAGCATTTCAGAGTATTGGAGTTACTACTA
  GCTCTGAAATGCAACAATGCATACAGATGGAACAGAAAAGATGGATTCAGTTATTTAAGGTTATCACCAA
  TCTAGTGAAAATGTTGAAGTCCAGAGACACGAGGAGAAATTTTTGTCCTCCAAACCACTGGCTGTCAGAA
  CAAGAAGATATTAAAGCAGATAAGGTCACTCAGCTCTATGTGCCAGCATCCAGACATGTGTGGAGGTTCC
  GGCGGATGGGGAGGATAGGCCCGCTGCAGTCCACCCTGGACGTGGGTTTGGAGTCCCCGCCGCTGTCTGT
  GTCTGAGGAAAGACAGCTTGCTGTCCTGACAGAGTTGCCTTTTGTGGTTCCATTTGAGGAACGAGTAAAG
  ATCTTTCAGAGGTTGATTTATGCAGATAAGCAAGAAGTTCAAGGAGATGGTCCATTTCTGGATGGAATTA
  ATGTCACAATAAGAAGAAATTACATTTATGAAGATGCTTATGACAAACTTTCTCCAGAAAATGAGCCTGA
  TTTGAAAAAGCGGATCCGTGTGCACTTGCTCAATGCCCATGGCCTGGATGAAGCTGGCATTGATGGTGGT
  GGTATTTTCAGAGAGTTTTTAAATGAACTACTGAAGTCAGGATTTAACCCCAACCAGGGGTTCTTTAAGA
  CTACTAATGAAGGGCTTCTGTACCCCAACCCGGCTGCTCAGATGCTTGTGGGAGATTCTTTTGCCAGACA
  TTACTACTTCCTAGGCAGAATGCTTGGAAAGGCTCTCTATGAGAACATGCTGGTGGAGCTGCCCTTTGCA
  GGCTTCTTTCTTTCCAAGTTGCTTGGAACCAGTGCCGACGTGGACATTCACCACCTCGCCTCCCTAGACC
  CTGAGGTGTATAAGAATTTGCTCTTTCTGAAGAGCTACGAAGACGATGTGGAGGAGCTTGGGCTGAACTT
  CACTGTGGTGAACAATGACCTGGGAGAGGCGCAGGTAGTTGAACTAAAATTCGGTGGGAAAGACATCCCT
  GTCACCAGCGCCAACCGGATTGCGTACATCCACTTGGTGGCAGACTACAGGCTGAACAGGCAGATCCGCC
  AGCACTGCCTGGCTTTCCGCCAGGGCCTTGCCAATGTCGTCAGCCTCGAGTGGCTCCGAATGTTTGATCA
  GCAAGAAATTCAGGTATTAATTTCTGGTGCACAAGTTCCCATAAGCCTAGAGGACCTAAAATCCTTTACA
  AACTATTCAGGAGGCTATTCTGCAGACCATCCTGTTATTAAGGTCTTCTGGAGAGTTGTGGAAGGGTTCA
  CTGATGAAGAAAAGCGCAAACTGCTGAAGTTTGTAACAAGCTGCTCTCGACCCCCTCTCTTGGGGTTTAA
  GGAGTTGTATCCCGCATTTTGTATTCACAACGGAGGCTCCGACCTTGAGCGGCTCCCCACAGCCAGCACC
  TGCATGAACCTGCTGAAGCTCCCCGAGTTCTATGACGAGACACTTTTGCGAAGTAAACTTCTCTATGCGA
  TTGAATGTGCCGCTGGCTTTGAGCTGAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215110 representing NM_014671
  Red=Cloning site Green=Tags(s)
  
  MFSFEGDFKTRPKVSLGGASRKEEKASLLHRTQEERRKREEERRRLKNAIIIQSFIRGYRDRKQQYSIQR
  SAFDRCATLSQSGGAFPIANGPNLTLLVRQLLFFYKQNEDSKRLIWLYQNLIKHSSLFVKQLDGSERLTC
  LFQIKRLMSLCCRLLQNCNDDSLNVALPMRMLEVFSSENTYLPVLQDASYVVSVIEQILHYMIHNGYYRS
  LYLLINSKLPSSIEYSDLSRVPIAKILLENVLKPLHFTYNSCPEGARQQVFTAFTEEFLAAPFTDQIFHF
  IIPALADAQTVFPYEPFLNALLLIESRCSRKSGGAPWLFYFVLTVGENYLGALSEEGLLVYLRVLQTFLS
  QLPVSPASASCHDSASDSEEESEEADKPSSPEDGRLSVSYITEECLKKLDTKQQTNTLLNLVWRDSASEE
  VFTTMASVCHTLMVQHRMMVPKVRLLYSLAFNARFLRHLWFLISSMSTRMITGSMVPLLQVISRGSPMSF
  EDSSRIIPLFYLFSSLFSHSLISIHDNEFFGDPIEVVGQRQSSMMPFTLEELIMLSRCLRDACLGIIKLA
  YPETKPEVREEYITAFQSIGVTTSSEMQQCIQMEQKRWIQLFKVITNLVKMLKSRDTRRNFCPPNHWLSE
  QEDIKADKVTQLYVPASRHVWRFRRMGRIGPLQSTLDVGLESPPLSVSEERQLAVLTELPFVVPFEERVK
  IFQRLIYADKQEVQGDGPFLDGINVTIRRNYIYEDAYDKLSPENEPDLKKRIRVHLLNAHGLDEAGIDGG
  GIFREFLNELLKSGFNPNQGFFKTTNEGLLYPNPAAQMLVGDSFARHYYFLGRMLGKALYENMLVELPFA
  GFFLSKLLGTSADVDIHHLASLDPEVYKNLLFLKSYEDDVEELGLNFTVVNNDLGEAQVVELKFGGKDIP
  VTSANRIAYIHLVADYRLNRQIRQHCLAFRQGLANVVSLEWLRMFDQQEIQVLISGAQVPISLEDLKSFT
  NYSGGYSADHPVIKVFWRVVEGFTDEEKRKLLKFVTSCSRPPLLGFKELYPAFCIHNGGSDLERLPTAST
  CMNLLKLPEFYDETLLRSKLLYAIECAAGFELS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014671
• ORF Size: 3249 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014671.1, NM_014671.2, NP_055486.1, NP_055486.2
• RefSeq Size: 5160
• RefSeq ORF: 3252
• Locus ID: 9690
• Domains: IQ, HECT
• Protein Families: Druggable Genome
• Protein Pathways: Ubiquitin mediated proteolysis
• MW: 123.7 kDa