PRODH2 (NM_021232) Human Tagged ORF Clone

CAT#: RC215144

PRODH2 (Myc-DDK-tagged)-Human proline dehydrogenase (oxidase) 2 (PRODH2)

Product Images

Western blot validation of overexpression lysate (Cat# LY402858) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215144 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PRODH2
• Synonyms: HSPOX1; HYPDH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215144 representing NM_021232
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCAGGGTAGTCTCAAACTCCTCGGTCTTGGCCTCCCAAAGTGTTGGGATTACAAACGTGAGAA
  CCGTATTTTCAAATGTATTCAATAACACTACAGCGTTTCCAATTTTAAGAGGAAGCAATTGCCACAAAAT
  CACTGCCCCTGGTCTGGGCAAGGGGCAGTTGGTGAACTTGCTGCCTCCAGAGAACCTTCCCTGGTGTGGA
  GGCAGCCAGGGACCCAGGATGCTCCGGACCTGTTACGTGCTCTGTTCCCAAGCTGGTCCCCCCTCCAGGG
  GCTGGCAGTCCCTGAGCTTTGATGGCGGGGCCTTCCACCTTAAGGGCACAGGAGAGCTGACACGGGCCTT
  GCTGGTTCTCCGGCTGTGTGCCTGGCCCCCACTCGTCACTCACGGGCTGTTGCTCCAGGCCTGGTCTCGG
  CGACTCCTGGGCTCCCGGCTCTCAGGCGCATTTCTCCGAGCATCCGTCTATGGGCAGTTTGTGGCTGGTG
  AGACAGCAGAGGAGGTGAAGGGCTGCGTGCAGCAGCTGCGGACCCTCAGCCTCCGACCACTGCTGGCAGT
  GCCCACTGAGGAGGAGCCGGACTCTGCTGCCAAGAGTGGTGAGGCGTGGTATGAGGGGAACCTCGGTGCT
  ATGCTGCGGTGTGTGGACCTGTCACGGGGCCTCCTGGAGCCCCCCAGCCTGGCTGAGGCCAGCCTCATGC
  AGCTGAAGGTGACGGCGCTGACCAGTACTCGGCTCTGTAAGGAGCTAGCCTCGTGGGTCAGAAGGCCGGG
  AGCCTCCTTGGAGCTGAGCCCCGAGAGGCTGGCTGAAGCTATGGACTCTGGGCAGAACCTCCAGGTCTCC
  TGCCTCAATGCTGAGCAGAACCAGCACCTCCGGGCCTCCCTCAGCCGCCTGCATCGGGTGGCACAGTATG
  CCCGGGCCCAGCACGTGCGGCTCCTGGTGGATGCGGAGTACACCTCACTGAACCCTGCGCTCTCGCTGCT
  GGTGGCTGCCCTGGCTGTGCGCTGGAACAGCCCGGGTGAAGGCGGGCCCTGGGTGTGGAACACCTACCAG
  GCCTGTCTAAAGGACACATTCGAGCGGCTGGGGAGGGATGCAGAGGCTGCGCACAGGGCCGGCCTGGCCT
  TCGGAGTGAAGCTGGTACGAGGTGCATATCTGGACAAGGAGAGAGCGGTGGCCCAGCTCCATGGGATGGA
  AGACCCCACTCAGCCTGACTATGAGGCCACCAGTCAGAGTTACAGCCGCTGCCTGGAACTGATGCTGACG
  CACGTGGCCCGCCATGGCCCCATGTGCCACCTCATGGTGGCTTCCCACAATGAGGAATCTGTTCGCCAGG
  CAACCAAGCGCATGTGGGAGCTGGGCATTCCTCTGGATGGGACTGTCTGTTTCGGACAACTTCTGGGCAT
  GTGTGACCACGTCTCTCTAGCACTGGGGCAGGCCGGCTATGTAGTGTATAAGTCCATTCCCTATGGCTCC
  TTGGAGGAGGTAATCCCCTACCTGATCCGGAGGGCCCAGGAGAACCGGAGCGTGCTTCAGGGTGCCCGCA
  GGGAACAGGAGCTGCTCAGCCAAGAACTGTGGCGGCGGCTGCTGCCAGGATGCCGAAGGATACCCCAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215144 representing NM_021232
  Red=Cloning site Green=Tags(s)
  
  MSPRVVSNSSVLASQSVGITNVRTVFSNVFNNTTAFPILRGSNCHKITAPGLGKGQLVNLLPPENLPWCG
  GSQGPRMLRTCYVLCSQAGPPSRGWQSLSFDGGAFHLKGTGELTRALLVLRLCAWPPLVTHGLLLQAWSR
  RLLGSRLSGAFLRASVYGQFVAGETAEEVKGCVQQLRTLSLRPLLAVPTEEEPDSAAKSGEAWYEGNLGA
  MLRCVDLSRGLLEPPSLAEASLMQLKVTALTSTRLCKELASWVRRPGASLELSPERLAEAMDSGQNLQVS
  CLNAEQNQHLRASLSRLHRVAQYARAQHVRLLVDAEYTSLNPALSLLVAALAVRWNSPGEGGPWVWNTYQ
  ACLKDTFERLGRDAEAAHRAGLAFGVKLVRGAYLDKERAVAQLHGMEDPTQPDYEATSQSYSRCLELMLT
  HVARHGPMCHLMVASHNEESVRQATKRMWELGIPLDGTVCFGQLLGMCDHVSLALGQAGYVVYKSIPYGS
  LEEVIPYLIRRAQENRSVLQGARREQELLSQELWRRLLPGCRRIPH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021232
• ORF Size: 1608 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021232.1, NP_067055.1
• RefSeq Size: 1677 bp
• RefSeq ORF: 1611 bp
• Locus ID: 58510
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 58.7 kDa
• Gene Summary: The protein encoded by this gene catalyzes the first step in the catabolism of trans-4-hydroxy-L-proline, an amino acid derivative obtained through food intake and collagen turnover. One of the downstream products of this catabolism is glyoxylate, which in people with disorders of glyoxalate metabolism can lead to an increase in oxalate levels and the formation of calcium-oxalate kidney stones. Therefore, this gene may serve as a therapeutic target against primary hyperoxalurias (PH). This gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme that catalyzes the first step in proline catabolism. [provided by RefSeq, Jan 2017]