Archaemetzincin 2 (AMZ2) (NM_001033571) Human Tagged ORF Clone

CAT#: RC215164

AMZ2 (Myc-DDK-tagged)-Human archaelysin family metallopeptidase 2 (AMZ2), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY422397) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215164 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AMZ2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215164 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAAATAATACGGCACTCCGAACAGACACTAAAAACAGCTCTCATCTCAAAGAACCCAGTGCTTGTAT
  CACAGTATGAGAAATTAGATGCTGGGGAACAACGTTTAATGAATGAAGCCTTCCAGCCAGCCAGTGATCT
  CTTTGGACCCATTACCTTGCATTCTCCATCAGATTGGATCACCTCCCACCCTGAGGCTCCCCAAGACTTT
  GAACAGTTCTTCAGTGATCCTTACAGAAAGACACCCTCTCCAAACAAACGCAGCATTTATATACAGTCCA
  TTGGCTCTCTAGGAAACACCAGAATTATCAGTGAAGAATATATTAAATGGCTCACGGGCTACTGTAAAGC
  ATATTTCTATGGCTTGAGAGTAAAACTCCTAGAACCAGTTCCTGTTTCTGTAACAAGATGTTCCTTTAGA
  GTCAATGAGAACACACACAACCTACAAATTCATGCAGGGGACATCCTGAAGTTCTTGAAAAAGAAGAAAC
  CTGAAGATGCCTTCTGTGTTGTGGGAATAACAATGATTGATCTTTACCCAAGAGACTCGTGGAATTTTGT
  CTTTGGACAGGCCTCTTTGACAGATGGTGTGGGGATATTCAGCTTTGCCAGGTATGGCAGTGATTTTTAT
  AGCATGCACTATAAAGGCAAAGTGAAGAAGCTCAAGAAAACATCTTCAAGTGACTATTCAATTTTCGACA
  ACTATTATATTCCAGAAATAACTAGTGTTTTACTACTTCGATCCTGTAAGACTTTAACCCATGAGATCGG
  ACACATATTTGGACTGCGACACTGCCAGTGGCTTGCATGCCTCATGCAAGGCTCCAACCACTTGGAAGAA
  GCTGACCGGCGCCCTCTAAACCTTTGCCCTATCTGTTTGCACAAGTTGCAGTGTGCTGTTGGCTTCAGCA
  TTGTAGAAAGATACAAAGCACTGGTGAGGTGGATTGATGATGAATCTTCTGACACACCTGGAGCAACTCC
  AGAACACAGTCACGAGGATAATGGGAATTTACCGAAACCCGTGGAAGCCTTTAAGGAATGGAAAGAGTGG
  ATAATAAAATGCCTGGCAGTTCTCCAAAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215164 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MQIIRHSEQTLKTALISKNPVLVSQYEKLDAGEQRLMNEAFQPASDLFGPITLHSPSDWITSHPEAPQDF
  EQFFSDPYRKTPSPNKRSIYIQSIGSLGNTRIISEEYIKWLTGYCKAYFYGLRVKLLEPVPVSVTRCSFR
  VNENTHNLQIHAGDILKFLKKKKPEDAFCVVGITMIDLYPRDSWNFVFGQASLTDGVGIFSFARYGSDFY
  SMHYKGKVKKLKKTSSSDYSIFDNYYIPEITSVLLLRSCKTLTHEIGHIFGLRHCQWLACLMQGSNHLEE
  ADRRPLNLCPICLHKLQCAVGFSIVERYKALVRWIDDESSDTPGATPEHSHEDNGNLPKPVEAFKEWKEW
  IIKCLAVLQK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033571
• ORF Size: 1080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033571.1, NP_001028743.1
• RefSeq Size: 1399 bp
• RefSeq ORF: 1083 bp
• Locus ID: 51321
• Cytogenetics: 17q24.2
• Protein Families: Druggable Genome
• MW: 41.3 kDa
• Gene Summary: The protein encoded by this gene is a zinc metalloprotease that displays some activity against angiotensin-3. The encoded protein is inhibited by the aminopeptidase inhibitor amastatin, as well as by the general inhibitors o-phenanthroline and batimastat. Defects in this gene may be associated with lung tumorigenesis. [provided by RefSeq, Oct 2016]