ARHGEF5 (NM_005435) Human Tagged ORF Clone

CAT#: RC215166

ARHGEF5 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5)

Product Images

Western blot validation of overexpression lysate (Cat# LY417305) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215166 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ARHGEF5
• Synonyms: GEF5; P60; TIM; TIM1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215166 representing NM_005435
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCTGAGGAGGCCCAGCGTGGAGCCTCTCCTCCCATCTCTGCCATAGAGGAATTCAGCATTATCC
  CTGAGGCTCCCATGAGGAGCAGCCAGGTCTCTGCCTTGGGGCTTGAAGCTCAAGAAGATGAGGACCCATC
  CTATAAGTGGAGAGAGGAACACAGACTCTCAGCAACTCAGCAGAGTGAGTTAAGGGATGTGTGTGACTAT
  GCGATTGAGACGATGCCCTCTTTTCCCAAGGAAGGTTCTGCAGATGTGGAGCCCAATCAGGAAAGCCTTG
  TGGCTGAGGCCTGTGACACTCCGGAACACTGGGAGGCAGTACCCCAGAGCCTAGCAGGCCGACAAGCAAG
  GACTCTAGCTCCCCCAGAGCTCTGGGCCTGCCCCATTCAGAGTGAGCATCTAGACATGGCCCCATTTTCC
  AGTGACCTGGGAAGCGAAGAAGAGGAGGTGGAATTTTGGCCAGGACTTACTTCTTTGACATTGGGATCTG
  GACAGGCAGAAGAAGAAGAGGAAACCTCTTCAGATAACTCTGGTCAGACCAGATATTATTCTCCCTGCGA
  AGAGCATCCTGCAGAGACCAACCAGAATGAAGGCTCTGAAAGTGGGACTATCAGGCAGGGGGAAGAGCTG
  CCACCTGAGGAGCTGCAGGAAAGTCAAGGGCTCTTGCATCCCCAGGAGGTCCAAGTTCTGGAGGAGCAGG
  GACAGCAGGAAGCAGGATTTCGGGGGGAAGGAACTCTGAGGGAGGATGTTTGTGCCGATGGGCTATTAGG
  GGAGGAACAGATGATAGAGCAGGTTAATGATGAAAAGGGAGAACAGAAGCAAAAACAGGAACAGGTACAA
  GATGTGATGCTTGGGAGACAAGGAGAAAGAATGGGGCTCACTGGGGAGCCAGAGGGTCTGAATGACGGTG
  AGTGGGAGCAGGAGGATATGGAGAGGAAGGCTCAGGGTCAGGGAGGTCCAGAACAGGGAGAAGAGAGGAA
  GAGGGAGCTGCAGGTGCCAGAAGAGAATAGGGCGGACTCTCAGGACGAAAAGAGTCAAACCTTTTTGGGA
  AAATCAGAGGAAGTAACTGGAAAGCAAGAAGATCATGGTATAAAGGAGAAAGGGGTGCCAGTCAGCGGGC
  AGGAGGCGAAAGAGCCAGAGAGTTGGGATGGGGGCAGGCTGGGGGCAGTGGGAAGAGCGAGGAGCAGGGA
  AGAGGAGAATGAGCATCATGGGCCTTCAATGCCCGCTCTGATAGCCCCTGAGGACTCTCCTCACTGTGAC
  CTGTTTCCAGGTGCCTCATATCTCATGACTCAGATTCCCGGGACTCAGACAGAGTCCAGGGCTGAGGAAC
  TGTCCCCCGCAGCTCTGTCTCCCTCGCTAGAGCCCATCAGGTGCTCTCACCAGCCCATTTCTCTACTGGG
  CTCCTTTTTGACTGAGGAGTCACCTGACAAGGAAATAGATCAAAACAGCCAGCAAGAGGAATCCAGGCTG
  AGGAAGGGAACAGTGTCCAGCCAAGGGACTGAGGTGGTCTTTGCCAGTGCATCTGTGACTCCTCCAAGGA
  CACCAGATTCAGCTCCTCCCAGTCCTGCTGAAGCCTACCCCATCACACCTGCCTCGGTATCTGCCAGGCC
  CCCAGTTGCCTTTCCCAGGAGGGAAACCTCTTGTGCTGCACGTGCTCCAGAAACTGCCAGTGCCCCTCTC
  TCAATGGATGACCCATCTCCCTGTGGGACTTCTGAGATGTGCCCGGCTGCCCTCTATGGCTTCCCCTCCA
  CCGGGACCAGCCCTCCGAGGCCCCCAGCCAACTCCACAGGCACCGTCCAGCACTTACGGAGTGACTCCTT
  CCCTGGTTCTCACAGGACAGAGCAGACTCCAGACCTGGTGGGAATGTTGCTTTCCTACTCCCACTCAGAG
  CTGCCCCAGAGGCCCCCCAAACCTGCCATCTACAGCTCTGTGACCCCAAGAAGGGACAGAAGGAGTGGTA
  GGGACTACAGCACCGTTTCAGCATCCCCTACTGCCTTATCCACGCTGAAGCAGGACTCTCAAGAATCCAT
  CTCAAATCTAGAGAGACCCAGCAGTCCTCCCAGCATCCAGCCCTGGGTCTCCCCACATAATCCAGCCTTT
  GCCACAGAGTCTCCCGCCTACGGTTCTTCCCCATCCTTTGTCTCCATGGAGGATGTGAGGATCCACGAAC
  CTCTGCCCCCTCCTCCCCCACAGAGGAGGGACACCCATCCCTCCGTGGTGGAGACAGATGGCCATGCTCG
  TGTAGTGGTTCCCACGCTGAAGCAGCATAGCCACCCTCCTCCATTGGCCCTAGGTTCAGGGCTGCATGCC
  CCCCATAAAGGCCCACTTCCCCAAGCCTCTGACCCCGCTGTGGCCAGGCAGCACCGACCTCTGCCATCTA
  CCCCAGACAGCTCCCACCATGCTCAGGCCACCCCCAGGTGGAGATACAACAAGCCGCTACCCCCTACCCC
  TGATTTGCCGCAGCCCCACCTTCCTCCCATTTCTGCTCCTGGTAGCTCAAGGATCTACAGGCCTCTACCC
  CCACTACCCATCATAGACCCTCCCACCGAACCACCCCCATTGCCCCCAAAGTCCAGGGGGAGGAGCAGGA
  GCACTCGGGGAGGACATATGAACTCAGGGGGTCATGCCAAAACAAGACCTGCTTGTCAAGACTGGACAGT
  CCCCCTCCCTGCCTCTGCTGGACGCACCTCCTGGCCCCCGGCCACAGCTAGATCAACAGAGTCTTTCACT
  TCCACCAGCAGGAGTAAGAGCGAAGTGTCCCCTGGCATGGCTTTCAGCAACATGACAAACTTCCTATGCC
  CCTCTTCCCCTACCACTCCCTGGACTCCGGAGCTCCAGGGACCCACCTCTAAGGATGAAGCAGGGGTCTC
  AGAACACCCTGAGGCCCCTGCGAGAGAACCTTTGAGAAGGACAACCCCTCAGCAAGGAGCCAGTGGCCCA
  GGGAGGTCACCTGTGGGCCAAGCAAGGCAGCCAGAAAAACCCAGCCATCTGCACCTGGAGAAGGCGTCCA
  GCTGGCCCCACAGGCGGGACTCAGGGAGGCCACCAGGGGACAGCAGTGGACAGGCTGTGGCTCCTAGTGA
  GGGGGCCAACAAGCACAAGGGCTGGAGCCGGCAGGGCCTGCGCAGACCTTCCATCTTGCCTGAGGGCTCT
  TCAGATTCAAGAGGTCCAGCCGTGGAGAAACATCCGGGACCCTCAGACACTGTTGTTTTTCGGGAGAAAA
  AACCAAAGGAGGTGATGGGAGGCTTTTCAAGACGCTGCTCCAAACTCATCAACTCCTCCCAGCTGCTTTA
  CCAGGAGTATAGTGATGTTGTCCTGAATAAGGAGATCCAGAGCCAGCAGCGGCTGGAGAGCCTGTCCGAG
  ACACCCGGGCCTAGCTCTCCGCGGCAGCCTCGGAAGGCCCTGGTCTCCTCCGAGTCGTACCTGCAGCGGC
  TCTCCATGGCCTCCAGCGGCTCCCTCTGGCAGGAAATCCCCGTGGTGCGCAACAGCACCGTGCTGCTCTC
  CATGACCCATGAAGACCAAAAGCTGCAAGAGGTCAAATTTGAGCTGATTGTGTCAGAGGCCTCCTACCTG
  CGCAGTCTAAACATAGCTGTGGATCATTTCCAACTTTCAACTTCACTCCGGGCCACACTTTCCAACCAGG
  AGCACCAATGGCTCTTCTCTCGTTTACAGGATGTGCGAGACGTCAGCGCCACGTTCCTTTCAGACCTGGA
  AGAGAACTTTGAGAACAATATCTTCTCCTTCCAAGTATGTGACGTAGTCCTGAACCACGCCCCAGACTTC
  CGCCGGGTCTACCTGCCTTATGTCACCAACCAGACCTATCAGGAACGCACCTTCCAGAGCCTGATGAATA
  GCAACAGCAATTTCCGGGAGGTCTTGGAGAAGCTGGAGAGCGACCCCGTCTGCCAGCGCCTTTCCCTCAA
  GTCCTTTCTGATTCTGCCCTTCCAACGCATCACCCGCCTCAAACTGCTGCTCCAGAACATTCTGAAGAGA
  ACACAGCCTGGCTCCTCGGAGGAGGCAGAGGCCACGAAGGCACACCACGCCCTGGAGCAGCTGATCCGGG
  ACTGCAATAACAATGTCCAGAGTATGCGACGGACAGAGGAACTAATCTACCTGAGCCAGAAGATTGAGTT
  TGAGTGCAAAATATTCCCGCTCATTTCTCAGTCACGCTGGCTGGTGAAAAGTGGGGAGCTGACAGCCTTG
  GAGTTCAGTGCTTCCCCAGGGCTACGAAGGAAGCTGAACACGCGTCCAGTCCACCTGCACCTCTTCAATG
  ACTGTCTGCTGCTGTCTCGGCCCCGAGAGGGTAGCCGATTCCTGGTATTTGACCATGCTCCCTTCTCCTC
  CATTCGGGGGGAAAAGTGTGAAATGAAGCTACATGGACCTCACAAAAACCTGTTCCGACTCTTTCTGCGG
  CAGAACACTCAGGGCGCCCAGGCCGAGTTCCTCTTCCGCACGGAGACTCAAAGTGAAAAGCTTCGGTGGA
  TCTCAGCCTTGGCCATGCCAAGAGAGGAGTTGGACCTTCTGGAGTGTTACAACTCCCCCCAGGTACAGTG
  CCTTCGAGCCTACAAGCCCCGAGAGAATGATGAATTGGCACTGGAGAAAGCCGACGTGGTGATGGTGACT
  CAGCAGAGCAGTGACGGCTGGCTGGAGGGCGTGAGGCTCTCAGACGGGGAGCGAGGCTGGTTTCCTGTGC
  AGCAGGTGGAGTTCATTTCCAACCCAGAGGTCCGTGCACAGAACCTGAAGGAAGCTCATCGAGTCAAGAC
  TGCCAAACTACAGCTGGTGGAACAGCAAGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215166 representing NM_005435
  Red=Cloning site Green=Tags(s)
  
  MEAEEAQRGASPPISAIEEFSIIPEAPMRSSQVSALGLEAQEDEDPSYKWREEHRLSATQQSELRDVCDY
  AIETMPSFPKEGSADVEPNQESLVAEACDTPEHWEAVPQSLAGRQARTLAPPELWACPIQSEHLDMAPFS
  SDLGSEEEEVEFWPGLTSLTLGSGQAEEEEETSSDNSGQTRYYSPCEEHPAETNQNEGSESGTIRQGEEL
  PPEELQESQGLLHPQEVQVLEEQGQQEAGFRGEGTLREDVCADGLLGEEQMIEQVNDEKGEQKQKQEQVQ
  DVMLGRQGERMGLTGEPEGLNDGEWEQEDMERKAQGQGGPEQGEERKRELQVPEENRADSQDEKSQTFLG
  KSEEVTGKQEDHGIKEKGVPVSGQEAKEPESWDGGRLGAVGRARSREEENEHHGPSMPALIAPEDSPHCD
  LFPGASYLMTQIPGTQTESRAEELSPAALSPSLEPIRCSHQPISLLGSFLTEESPDKEIDQNSQQEESRL
  RKGTVSSQGTEVVFASASVTPPRTPDSAPPSPAEAYPITPASVSARPPVAFPRRETSCAARAPETASAPL
  SMDDPSPCGTSEMCPAALYGFPSTGTSPPRPPANSTGTVQHLRSDSFPGSHRTEQTPDLVGMLLSYSHSE
  LPQRPPKPAIYSSVTPRRDRRSGRDYSTVSASPTALSTLKQDSQESISNLERPSSPPSIQPWVSPHNPAF
  ATESPAYGSSPSFVSMEDVRIHEPLPPPPPQRRDTHPSVVETDGHARVVVPTLKQHSHPPPLALGSGLHA
  PHKGPLPQASDPAVARQHRPLPSTPDSSHHAQATPRWRYNKPLPPTPDLPQPHLPPISAPGSSRIYRPLP
  PLPIIDPPTEPPPLPPKSRGRSRSTRGGHMNSGGHAKTRPACQDWTVPLPASAGRTSWPPATARSTESFT
  STSRSKSEVSPGMAFSNMTNFLCPSSPTTPWTPELQGPTSKDEAGVSEHPEAPAREPLRRTTPQQGASGP
  GRSPVGQARQPEKPSHLHLEKASSWPHRRDSGRPPGDSSGQAVAPSEGANKHKGWSRQGLRRPSILPEGS
  SDSRGPAVEKHPGPSDTVVFREKKPKEVMGGFSRRCSKLINSSQLLYQEYSDVVLNKEIQSQQRLESLSE
  TPGPSSPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEVKFELIVSEASYL
  RSLNIAVDHFQLSTSLRATLSNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDF
  RRVYLPYVTNQTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKR
  TQPGSSEEAEATKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGELTAL
  EFSASPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLR
  QNTQGAQAEFLFRTETQSEKLRWISALAMPREELDLLECYNSPQVQCLRAYKPRENDELALEKADVVMVT
  QQSSDGWLEGVRLSDGERGWFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQA
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005435
• ORF Size: 4791 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005435.3, NP_005426.2
• RefSeq Size: 5505
• RefSeq ORF: 4794
• Locus ID: 7984
• Domains: RhoGEF, SH3, PH
• Protein Families: Druggable Genome
• MW: 176.8 kDa
• Gene Summary: Rho GTPases play a fundamental role in numerous cellular processes initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein may be involved in the control of cytoskeletal organization. [provided by RefSeq, Jul 2008]