Calpain 9 (CAPN9) (NM_006615) Human Tagged ORF Clone

CAT#: RC215171

CAPN9 (Myc-DDK-tagged)-Human calpain 9 (CAPN9), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY401979) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215171 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CAPN9 protein (Cat# TP315171). The protein was produced from HEK293T cells transfected with CAPN9 cDNA clone (Cat# RC215171) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CAPN9
• Synonyms: GC36; nCL-4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215171 representing NM_006615
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCTTACCTCTACCGGGCCCCAGGGCCTCAGGCACACCCGGTTCCCAAGGACGCCCGGATCACCCACT
  CCTCAGGCCAGAGCTTTGAGCAAATGAGGCAGGAGTGCCTGCAGAGAGGCACCCTGTTTGAGGATGCAGA
  CTTCCCAGCCAGCAATTCCTCCCTGTTCTACAGTGAGAGGCCGCAGATCCCCTTTGTGTGGAAACGACCA
  GGGGAAATCGTGAAAAACCCAGAATTCATTCTTGGAGGGGCCACCAGGACTGATATCTGCCAGGGAGAGC
  TGGGAGACTGCTGGCTATTAGCCGCCATCGCCTCCCTTACGCTTAATCAAAAAGCACTGGCCAGAGTCAT
  CCCCCAGGACCAAAGATTTGGCCCTGGTTATGCCGGGATATTCCATTTCCAGTTCTGGCAGCACAGTGAG
  TGGCTGGACGTGGTGATCGATGACCGCCTGCCCACCTTCAGGGACCGCTTGGTTTTCCTCCACTCTGCCG
  ACCACAACGAGTTCTGGAGCGCCTTGCTGGAAAAAGCCTACGCCAAGCTAAATGGGAGCTATGAAGCTCT
  GAAGGGAGGCAGCGCCATCGAGGCCATGGAAGACTTCACTGGGGGTGTGGCAGAGACCTTCCAAACTAAA
  GAGGCCCCCGAGAACTTCTATGAGATTCTAGAGAAGGCTTTGAAGAGAGGCTCCCTGCTGGGCTGCTTCA
  TTGATACCAGAAGTGCTGCAGAATCTGAGGCCCGGACGCCGTTTGGTCTTATTAAGGGTCATGCCTACAG
  TGTAACGGGAATTGACCAGGTAAGCTTCCGAGGCCAGAGAATCGAGCTCATCCGAATCCGGAACCCTTGG
  GGCCAGGTTGAGTGGAACGGGTCGTGGAGCGACAGTTCTCCGGAGTGGCGTTCTGTTGGTCCAGCTGAGC
  AGAAGCGTCTGTGTCACACTGCTCTGGATGATGGGGAATTCTGGATGGCATTTCAGGACTTCAAGGCCCA
  CTTTGATAAAGTGGAGATCTGCAACCTCACTCCCGATGCCCTGGAGGAAGACGCGATCCACAAATGGGAG
  GTGACGGTCCATCAGGGAAGCTGGGTTCGCGGCTCCACGGCTGGGGGCTGCCGCAATTTCCTGGATACCT
  TTTGGACCAATCCACAAATAAAATTGTCTCTGACTGAGAAAGATGAGGGGCAGGAGGAGTGTAGTTTCCT
  TGTAGCCCTGATGCAGAAAGATAGAAGGAAACTCAAGAGATTTGGTGCCAATGTGCTGACAATCGGCTAT
  GCCATTTATGAGTGCCCTGACAAAGACGAACACCTGAACAAAGACTTCTTCAGATACCACGCTTCTCGGG
  CCAGAAGCAAGACGTTCATCAACCTGAGAGAAGTCTCCGACCGGTTCAAGCTGCCCCCTGGGGAGTACAT
  CCTGATTCCCAGCACTTTTGAGCCCCACCAGGAAGCTGATTTCTGTCTGAGAATCTTTTCAGAGAAAAAA
  GCCATTACCCGGGATATGGATGGAAATGTAGACATTGACCTTCCTGAGCCTCCAAAGCCAACTCCACCTG
  ACCAGGAGACAGAGGAGGAGCAGCGGTTTCGGGCTCTGTTTGAACAAGTCGCTGGTGAGGACATGGAGGT
  GACAGCAGAGGAACTTGAGTATGTTTTAAATGCTGTGCTGCAAAAGAAAAAGGACATCAAATTCAAGAAG
  CTAAGCCTGATCTCCTGTAAAAACATCATTTCCCTGATGGACACCAGCGGCAATGGGAAGCTGGAGTTTG
  ATGAATTCAAAGTGTTCTGGGACAAGCTGAAGCAGTGGATTAACCTTTTCCTTCGGTTTGATGCTGACAA
  GTCCGGCACCATGTCTACCTATGAACTACGGACTGCACTGAAAGCTGCAGGCTTTCAGCTGAGCAGCCAC
  CTCCTGCAGCTGATTGTGCTCAGGTATGCGGATGAGGAGCTCCAGCTGGACTTCGATGACTTCCTCAACT
  GCCTGGTCCGGCTGGAGAATGCGAGCCGGGTGTTCCAGGCTCTCAGTACAAAGAACAAGGAGTTCATTCA
  TCTCAATATAAATGAGTTCATCCATTTGACAATGAACATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215171 representing NM_006615
  Red=Cloning site Green=Tags(s)
  
  MPYLYRAPGPQAHPVPKDARITHSSGQSFEQMRQECLQRGTLFEDADFPASNSSLFYSERPQIPFVWKRP
  GEIVKNPEFILGGATRTDICQGELGDCWLLAAIASLTLNQKALARVIPQDQRFGPGYAGIFHFQFWQHSE
  WLDVVIDDRLPTFRDRLVFLHSADHNEFWSALLEKAYAKLNGSYEALKGGSAIEAMEDFTGGVAETFQTK
  EAPENFYEILEKALKRGSLLGCFIDTRSAAESEARTPFGLIKGHAYSVTGIDQVSFRGQRIELIRIRNPW
  GQVEWNGSWSDSSPEWRSVGPAEQKRLCHTALDDGEFWMAFQDFKAHFDKVEICNLTPDALEEDAIHKWE
  VTVHQGSWVRGSTAGGCRNFLDTFWTNPQIKLSLTEKDEGQEECSFLVALMQKDRRKLKRFGANVLTIGY
  AIYECPDKDEHLNKDFFRYHASRARSKTFINLREVSDRFKLPPGEYILIPSTFEPHQEADFCLRIFSEKK
  AITRDMDGNVDIDLPEPPKPTPPDQETEEEQRFRALFEQVAGEDMEVTAEELEYVLNAVLQKKKDIKFKK
  LSLISCKNIISLMDTSGNGKLEFDEFKVFWDKLKQWINLFLRFDADKSGTMSTYELRTALKAAGFQLSSH
  LLQLIVLRYADEELQLDFDDFLNCLVRLENASRVFQALSTKNKEFIHLNINEFIHLTMNI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006615
• ORF Size: 2070 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006615.1, NM_006615.2, NP_006606.1
• RefSeq Size: 2362 bp
• RefSeq ORF: 2073 bp
• Locus ID: 10753
• Domains: Calpain_III, EFh
• Protein Families: Druggable Genome, Protease
• MW: 78.9 kDa
• Gene Summary: Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]