Kv beta 1 (KCNAB1) (NM_003471) Human Tagged ORF Clone

CAT#: RC215331

KCNAB1 (Myc-DDK-tagged)-Human potassium voltage-gated channel, shaker-related subfamily, beta member 1 (KCNAB1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY418655) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215331 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCNAB1
• Synonyms: AKR6A3; hKvb3; hKvBeta3; KCNA1B; KV-BETA-1; Kvb1.3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215331 representing NM_003471
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCTGTATAAACCTGCCTGTGCAGACATCCCGAGCCCCAAGCTGGGTCTGCCAAAATCCAGTGAAT
  CGGCTCTAAAATGTAGATGGCACCTAGCAGTGACCAAGACTCAGCCTCAGGCGGCCTGCAAACCTGTGAG
  GCCCAGTGGAGCAGCCGAACAGAAATATGTGGAAAAGTTTCTACGTGTTCATGGAATTTCGTTGCAGGAA
  ACCACCAGAGCAGAGACGGGCATGGCATACAGGAATCTTGGAAAATCAGGACTCAGAGTTTCTTGCTTGG
  GTCTTGGAACATGGGTGACATTTGGAGGTCAAATTTCAGATGAGGTTGCTGAACGGCTGATGACCATCGC
  CTATGAAAGTGGTGTTAACCTCTTTGATACTGCCGAAGTCTATGCTGCTGGAAAGGCTGAAGTGATTCTG
  GGGAGCATCATCAAGAAGAAAGGCTGGAGGAGGTCCAGTCTGGTCATAACAACCAAACTCTACTGGGGTG
  GAAAAGCTGAAACAGAAAGAGGGCTGTCAAGAAAGCATATTATTGAAGGATTGAAGGGCTCCCTCCAGAG
  GCTGCAGCTCGAGTATGTGGATGTGGTCTTTGCAAATCGACCGGACAGTAACACTCCCATGGAAGAAATT
  GTCCGAGCCATGACACATGTGATAAACCAAGGCATGGCGATGTACTGGGGCACCTCGAGATGGAGTGCTA
  TGGAGATCATGGAAGCCTATTCTGTAGCAAGACAGTTCAATATGATCCCACCGGTCTGTGAACAAGCTGA
  GTACCATCTTTTCCAGAGAGAGAAAGTGGAGGTCCAGCTGCCAGAGCTCTACCACAAAATAGGTGTTGGC
  GCAATGACATGGTCTCCACTTGCCTGTGGAATCATCTCAGGAAAATACGGAAACGGGGTGCCTGAAAGTT
  CCAGGGCTTCACTGAAGTGCTACCAGTGGTTGAAAGAAAGAATTGTAAGTGAAGAAGGGAGAAAACAGCA
  AAACAAGCTAAAAGACCTTTCCCCAATTGCGGAGCGTCTGGGATGCACACTACCTCAGCTAGCTGTTGCG
  TGGTGCCTGAGAAATGAAGGTGTGAGTTCTGTGCTCCTGGGATCATCCACTCCTGAACAACTCATTGAAA
  ACCTTGGTGCCATTCAGGTTCTCCCAAAGATGACATCACATGTGGTAAATGAGATTGATAACATACTGCG
  CAACAAGCCCTACAGCAAGAAGGACTATAGATCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215331 representing NM_003471
  Red=Cloning site Green=Tags(s)
  
  MHLYKPACADIPSPKLGLPKSSESALKCRWHLAVTKTQPQAACKPVRPSGAAEQKYVEKFLRVHGISLQE
  TTRAETGMAYRNLGKSGLRVSCLGLGTWVTFGGQISDEVAERLMTIAYESGVNLFDTAEVYAAGKAEVIL
  GSIIKKKGWRRSSLVITTKLYWGGKAETERGLSRKHIIEGLKGSLQRLQLEYVDVVFANRPDSNTPMEEI
  VRAMTHVINQGMAMYWGTSRWSAMEIMEAYSVARQFNMIPPVCEQAEYHLFQREKVEVQLPELYHKIGVG
  AMTWSPLACGIISGKYGNGVPESSRASLKCYQWLKERIVSEEGRKQQNKLKDLSPIAERLGCTLPQLAVA
  WCLRNEGVSSVLLGSSTPEQLIENLGAIQVLPKMTSHVVNEIDNILRNKPYSKKDYRS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003471
• ORF Size: 1224 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003471.2, NM_003471.3, NP_003462.2
• RefSeq Size: 3301
• RefSeq ORF: 1227
• Locus ID: 7881
• Domains: aldo_ket_red
• Protein Families: Druggable Genome, Ion Channels: Other
• MW: 45.3 kDa
• Gene Summary: Potassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member includes distinct isoforms which are encoded by alternatively spliced transcript variants of this gene. Some of these isoforms are beta subunits, which form heteromultimeric complexes with alpha subunits and modulate the activity of the pore-forming alpha subunits. [provided by RefSeq, Apr 2015]