C9orf127 (TMEM8B) (NM_001042590) Human Tagged ORF Clone

CAT#: RC215347

TMEM8B (Myc-DDK-tagged)-Human transmembrane protein 8B (TMEM8B), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420998) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215347 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TMEM8B
• Synonyms: C9orf127; FP588; LINC00950; NAG-5; NAG5; NGX6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215347 representing NM_001042590
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATGCCCCAGTCCCTGGGCAACCAGCCACTGCCCCCAGAACCGCCATCCCTTGGAACCCCTGCGG
  AGGGGCCTGGGACCACGTCCCCACCCGAGCACTGCTGGCCAGTGCGCCCGACTCTGCGCAACGAGCTGGA
  CACCTTCTCTGTCCACTTCTACATCTTCTTTGGCCCAAGTGTGGCCCTTCCCCCTGAGCGCCCAGCCGTG
  TTCGCCATGAGGCTGTTGCCAGTGCTGGACAGTGGAGGCGTCCTCAGCCTGGAGCTCCAGCTCAATGCGA
  GCTCCGTGCGCCAGGAAAACGTGACGGTGTTTGGATGCTTGACTCACGAGGTGCCCTTGAGCCTGGGGGA
  TGCAGCAGTGACCTGTTCCAAAGAGTCCCTGGCCGGCTTCCTCCTCTCTGTCAGTGCCACCACCAGGGTT
  GCCAGGCTGCGAATCCCATTCCCGCAGACGGGGACCTGGTTCCTGGCCCTCCGCTCCCTGTGCGGGGTGG
  GGCCTCGGTTCGTGCGGTGCCGCAACGCGACGGCCGAGGTGCGGATGCGCACCTTCCTGTCCCCATGCGT
  GGACGACTGCGGGCCCTACGGCCAGTGCAAGCTGCTGCGCACACACAATTATCTGTACGCAGCCTGCGAG
  TGCAAGGCCGGGTGGAGAGGCTGGGGCTGCACCGACAGTGCAGATGCGCTCACCTATGGATTCCAGCTGC
  TGTCCACACTCCTGCTCTGCCTGAGCAACCTCATGTTTCTGCCACCTGTGGTCCTGGCCATTCGGAGTCG
  ATATGTGCTGGAAGCTGCAGTCTACACCTTCACCATGTTCTTCTCCACGTTCTATCATGCCTGTGACCAG
  CCAGGCATCGTGGTTTTCTGCATCATGGACTACGATGTGCTGCAGTTCTGTGATTTCCTGGGCTCCTTAA
  TGTCCGTGTGGGTCACTGTCATTGCCATGGCTCGTTTACAGCCCGTGGTCAAGCAGGTGCTGTATTTGCT
  GGGAGCTATGCTGCTGTCCATGGCTCTGCAGCTTGACCGACATGGACTCTGGAACCTGCTTGGACCCAGT
  CTCTTCGCCCTGGGGATCTTGGCCACAGCCTGGACAGTACGCAGCGTCCGCCGCCGGCACTGCTACCCAC
  CCACGTGGCGCCGCTGGCTTTTCTACTTGTGCCCTGGCAGCCTTATTGCAGGCAGTGCCGTCCTGCTTTA
  TGCTTTTGTGGAGACCCGGGACAACTACTTCTACATTCACAGCATTTGGCATATGCTCATTGCGGGCAGT
  GTGGGCTTCCTGCTGCCCCCTCGTGCCAAGACTGACCACGGGGTCCCATCTGGAGCCCGGGCCCGGGGCT
  GTGGTTACCAGCTATGCATCAACGAGCAGGAGGAGCTGGGCCTCGTGGGCCCAGGAGGGGCCACTGTCAG
  CAGCATCTGTGCCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215347 representing NM_001042590
  Red=Cloning site Green=Tags(s)
  
  MNMPQSLGNQPLPPEPPSLGTPAEGPGTTSPPEHCWPVRPTLRNELDTFSVHFYIFFGPSVALPPERPAV
  FAMRLLPVLDSGGVLSLELQLNASSVRQENVTVFGCLTHEVPLSLGDAAVTCSKESLAGFLLSVSATTRV
  ARLRIPFPQTGTWFLALRSLCGVGPRFVRCRNATAEVRMRTFLSPCVDDCGPYGQCKLLRTHNYLYAACE
  CKAGWRGWGCTDSADALTYGFQLLSTLLLCLSNLMFLPPVVLAIRSRYVLEAAVYTFTMFFSTFYHACDQ
  PGIVVFCIMDYDVLQFCDFLGSLMSVWVTVIAMARLQPVVKQVLYLLGAMLLSMALQLDRHGLWNLLGPS
  LFALGILATAWTVRSVRRRHCYPPTWRRWLFYLCPGSLIAGSAVLLYAFVETRDNYFYIHSIWHMLIAGS
  VGFLLPPRAKTDHGVPSGARARGCGYQLCINEQEELGLVGPGGATVSSICAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001042590
• ORF Size: 1416 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001042590.1, NM_001042590.2, NP_001036055.1
• RefSeq Size: 3747
• RefSeq ORF: 2775
• Locus ID: 51754
• Protein Families: Transmembrane
• MW: 51.8 kDa