PCTAIRE3 (CDK18) (NM_212503) Human Tagged ORF Clone

CAT#: RC215371

CDK18 (Myc-DDK-tagged)-Human cyclin-dependent kinase 18 (CDK18), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY403955) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215371 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDK18
• Synonyms: PCTAIRE; PCTAIRE3; PCTK3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215371 representing NM_212503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATCATGAACAAGATGAAGAACTTTAAGCGCCGTTTCTCCCTGTCAGTGCCCCGCACTGAGACCATTG
  AAGAATCCTTGGCTGAATTCACGGAGCAATTCAACCAGCTCCACAACCGGCGGAATGAGAACTTGCAGCT
  CGGTCCTCTTGGCAGAGACCCCCCGCAGGAGTGCAGCACCTTCTCCCCAACAGACAGCGGGGAGGAGCCG
  GGGCAGCTCTCCCCTGGCGTGCAGTTCCAGCGGCGGCAGAACCAGCGCCGCTTCTCCATGGAGGTGAGGG
  CCTCTGGAGCTCTGCCCCGGCAGGTGGCAGGATGCACGCACAAGGGTGTGCACAGGAGGGCAGCTGCCTT
  ACAGCCAGACTTTGACGTCAGCAAGAGGCTCTCTCTGCCCATGGATATCCGCCTGCCCCAGGAATTCCTA
  CAGAAGCTACAGATGGAGAGCCCAGATCTGCCCAAGCCGCTCAGCCGCATGTCCCGCCGGGCCTCCCTGT
  CAGACATTGGCTTTGGGAAACTGGAAACATACGTGAAACTGGACAAACTGGGAGAGGGCACCTATGCCAC
  AGTCTTCAAAGGGCGCAGCAAACTGACGGAGAACCTTGTGGCCCTGAAAGAGATCCGGCTGGAGCACGAG
  GAGGGAGCGCCCTGCACTGCCATCCGAGAGGTGTCTCTGCTGAAGAACCTGAAGCACGCCAATATTGTGA
  CCCTGCATGACCTCATCCACACAGATCGGTCCCTCACCCTGGTGTTTGAGTACCTGGACAGTGACCTGAA
  GCAGTATCTGGACCACTGTGGGAACCTCATGAGCATGCACAACGTCAAGATTTTCATGTTCCAGCTGCTC
  CGGGGCCTCGCCTACTGTCACCACCGCAAGATCCTGCACCGGGACCTGAAGCCCCAGAACCTGCTCATCA
  ACGAGAGGGGGGAGCTGAAGCTGGCCGACTTTGGACTGGCCAGGGCCAAGTCAGTGCCCACAAAGACTTA
  CTCCAATGAGGTGGTGACCCTGTGGTACAGGCCCCCCGATGTGCTGCTGGGATCCACAGAGTACTCCACC
  CCCATTGATATGTGGGGCGTGGGCTGCATCCACTACGAGATGGCCACAGGGAGGCCCCTCTTCCCGGGCT
  CCACAGTCAAGGAGGAGCTGCACCTCATCTTTCGCCTCCTCGGGACCCCCACAGAAGAGACGTGGCCCGG
  CGTGACCGCCTTCTCTGAGTTCCGCACCTACAGCTTCCCCTGCTACCTCCCGCAGCCGCTCATCAACCAC
  GCGCCCAGGTTGGATACGGATGGCATCCACCTCCTGAGCAGCCTGCTCCTGTATGAATCCAAGAGTCGCA
  TGTCAGCAGAGGCTGCCCTGAGTCACTCCTACTTCCGGTCTCTGGGAGAGCGTGTGCACCAGCTTGAAGA
  CACTGCCTCCATCTTCTCCCTGAAGGAGATCCAGCTCCAGAAGGACCCAGGCTACCGAGGCTTGGCCTTC
  CAGCAGCCAGGACGAGGGAAGAACAGGCGGCAGAGCATCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215371 representing NM_212503
  Red=Cloning site Green=Tags(s)
  
  MIMNKMKNFKRRFSLSVPRTETIEESLAEFTEQFNQLHNRRNENLQLGPLGRDPPQECSTFSPTDSGEEP
  GQLSPGVQFQRRQNQRRFSMEVRASGALPRQVAGCTHKGVHRRAAALQPDFDVSKRLSLPMDIRLPQEFL
  QKLQMESPDLPKPLSRMSRRASLSDIGFGKLETYVKLDKLGEGTYATVFKGRSKLTENLVALKEIRLEHE
  EGAPCTAIREVSLLKNLKHANIVTLHDLIHTDRSLTLVFEYLDSDLKQYLDHCGNLMSMHNVKIFMFQLL
  RGLAYCHHRKILHRDLKPQNLLINERGELKLADFGLARAKSVPTKTYSNEVVTLWYRPPDVLLGSTEYST
  PIDMWGVGCIHYEMATGRPLFPGSTVKEELHLIFRLLGTPTEETWPGVTAFSEFRTYSFPCYLPQPLINH
  APRLDTDGIHLLSSLLLYESKSRMSAEAALSHSYFRSLGERVHQLEDTASIFSLKEIQLQKDPGYRGLAF
  QQPGRGKNRRQSIF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_212503
• ORF Size: 1512 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212503.1, NM_212503.2, NP_997668.1
• RefSeq Size: 3165 bp
• RefSeq ORF: 1515 bp
• Locus ID: 5129
• Cytogenetics: 1q32.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 57.4 kDa
• Gene Summary: ''