CHFR (NM_018223) Human Tagged ORF Clone

CAT#: RC215377

CHFR (Myc-DDK-tagged)-Human checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4

Product Images

Western blot validation of overexpression lysate (Cat# LY413203) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215377 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CHFR
• Synonyms: RNF116; RNF196
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215377 representing NM_018223
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGCCCGAGGAAGGCAAGCAGTCGCCGCCGCCGCAGCCCTGGGGACGGCTCCTGCGTCTGGGCG
  CGGAGGAGGGCGAGCCGCACGTCCTCCTGAGGAAGCGGGAGTGGACCATCGGGCGGAGACGAGGTTGCGA
  CCTTTCCTTCCCCAGCAATAAACTGGTCTCTGGAGATCACTGTAGAATTGTAGTGGATGAAAAATCAGGT
  CAGGTGACACTGGAAGATACCAGCACCAGTGGAACAGTGATTAACAAGCTGAAGGTTGTTAAGAAGCAGA
  CATGCCCTTTACAGACTGGGGATGTCATCTACTTGGTGTACAGGAAGAATGAACCGGAACACAACGTGGC
  ATACCTCTATGAATCTTTAAGTGAAAAGCAAGGCATGACACAAGAATCCTTTGAGATGGTGCCTTGCTGT
  GTTGCCCAGGCTGGTCTAAAACTCCTGGGATCAAGTGATCCTCCCACCTTGGCCTCCCAAAGTATTGTGA
  TTACAGGGTCTGGGGGTGGTGGCATCTCCCCTAAAGGAAGTGGTCCCTCTGTGGCAAGTGATGAAGTCTC
  CAGCTTTGCCTCAGCTCTCCCAGACAGAAAGACTGCGTCCTTTTCGTCGTTGGAACCCCAGGATCAGGAG
  GATTTGGAGCCCGTGAAGAAGAAAATGAGAGGAGATGGGGACCTTGACCTGAACGGGCAGTTGTTGGTCG
  CACAACCGCGTAGAAATGCCCAAACCGTCCACGAGGACGTCAGAGCAGCGGCTGGGAAGCCAGACAAGAT
  GGAGGAGACGCTGACATGCATCATCTGCCAGGACCTGCTGCACGACTGCGTGAGTTTGCAGCCCTGCATG
  CACACGTTCTGCGCGGCTTGCTACTCGGGCTGGATGGAGCGCTCGTCCCTGTGTCCTACCTGCCGCTGTC
  CCGTGGAGCGGATCTGTAAAAACCACATCCTCAACAACCTCGTGGAAGCATACCTCATCCAGCATCCAGA
  CAAGAGTCGCAGTGAAGAAGATGTGCAAAGTATGGATGCCAGGAATAAAATCACTCAAGACATGCTGCAG
  CCCAAAGTCAGGCGGTCTTTTTCTGATGAAGAAGGGAGTTCAGAGGACCTGCTGGAGCTGTCAGACGTTG
  ACAGTGAGTCCTCAGACATTAGCCAGCCATACGTCGTGTGCCGGCAGTGTCCTGAGTACAGAAGGCAGGC
  GGCGCAGCCTCCCCACTGCCCAGCACCCGAGGGCGAGCCAGGAGCCCCACAGGCCCTGGGGGATGCACCC
  TCCACGTCCGTCAGCCTGACGACAGCAGTCCAGGATTACGTGTGCCCTCTGCAAGGAAGCCACGCCCTGT
  GCACCTGCTGCTTCCAGCCCATGCCCGACCGGAGAGCGGAGCGCGAGCAGGACCCGCGTGTCGCCCCTCA
  GCAGTGTGCGGTCTGCCTGCAGCCTTTCTGCCACCTGTACTGGGGCTGCACCCGGACCGGCTGCTACGGC
  TGCCTGGCCCCGTTTTGTGAGCTCAACCTGGGTGACAAGTGTCTGGACGGCGTGCTGAACAACAACAGCT
  ACGAGTCAGACATCCTGAAGAATTACCTGGCAACCAGAGGTTTGACATGGAAAAACATGTTGACCGAGAG
  CCTCGTGGCTCTCCAGCGGGGAGTGTTTCTGCTGTCTGATTACAGAGTCACGGGAGACACCGTTCTGTGT
  TACTGCTGTGGCCTGCGCAGCTTCCGTGAGCTGACCTATCAGTATCGGCAGAACATTCCTGCTTCCGAGT
  TGCCAGTGGCCGTAACATCCCGTCCTGACTGCTACTGGGGCCGTAACTGCCGCACTCAGGTGAAAGCTCA
  CCACGCCATGAAATTCAATCATATCTGTGAACAGACAAGGTTCAAAAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215377 representing NM_018223
  Red=Cloning site Green=Tags(s)
  
  MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSG
  QVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEMVPCC
  VAQAGLKLLGSSDPPTLASQSIVITGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQE
  DLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCM
  HTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQ
  PKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAP
  STSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYG
  CLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLC
  YCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018223
• ORF Size: 1869 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018223.1, NM_018223.2, NP_060693.1, NP_060693.2
• RefSeq Size: 3138
• RefSeq ORF: 1872
• Locus ID: 55743
• Domains: FHA, RING
• Protein Families: Druggable Genome
• MW: 69 kDa
• Gene Summary: This gene encodes an E3 ubiquitin-protein ligase required for the maintenance of the antephase checkpoint that regulates cell cycle entry into mitosis and, therefore, may play a key role in cell cycle progression and tumorigenesis. The encoded protein has an N-terminal forkhead-associated domain, a central RING-finger domain, and a cysteine-rich C-terminal region. Alternatively spliced transcript variants that encode different protein isoforms have been described. [provided by RefSeq, Mar 2014]