Neurexin 1 (NRXN1) (NM_138735) Human Tagged ORF Clone

CAT#: RC215381

NRXN1 (Myc-DDK-tagged)-Human neurexin 1 (NRXN1), transcript variant beta

Product Images

Western blot validation of overexpression lysate (Cat# LY403367) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215381 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NRXN1
• Synonyms: Hs.22998; PTHSL2; SCZD17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215381 representing NM_138735
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACCAGAGGATGCTCCGGTGCGGCGCCGAGCTGGGCTCGCCCGGGGGCGGCGGCGGCGGCGGCGGCG
  GCGGCGGCGCAGGGGGGCGCCTGGCCCTGCTTTGGATAGTCCCGCTCACCCTCAGCGGCCTCCTAGGAGT
  GGCGTGGGGGGCATCCAGTTTGGGAGCGCACCACATCCACCATTTCCATGGCAGCAGCAAGCATCATTCA
  GTGCCTATTGCAATCTACAGGTCACCGGCATCCTTGCGAGGCGGACACGCTGGGACGACATATATCTTTA
  GCAAAGGTGGTGGACAAATCACGTATAAGTGGCCTCCTAATGACCGACCCAGTACACGAGCAGACAGACT
  GGCCATAGGTTTTAGCACTGTTCAGAAAGAAGCCGTATTGGTGCGAGTGGACAGTTCTTCAGGCTTGGGT
  GACTACCTAGAACTGCATATACACCAGGGAAAAATTGGAGTTAAGTTTAATGTTGGGACAGATGACATCG
  CCATTGAAGAATCCAATGCAATCATTAATGATGGGAAATACCATGTAGTTCGTTTCACGAGGAGTGGTGG
  CAATGCCACGTTGCAGGTGGACAGCTGGCCAGTGATCGAGCGCTACCCTGCAGGGCGTCAGCTCACAATC
  TTCAATAGCCAAGCAACCATAATAATTGGCGGGAAAGAGCAGGGCCAGCCCTTCCAGGGCCAGCTCTCTG
  GGCTGTACTACAATGGCTTGAAAGTTCTGAATATGGCAGCCGAAAACGATGCCAACATCGCCATAGTGGG
  AAATGTGAGACTGGTTGGTGAAGTGCCTTCCTCTATGACAACTGAGTCAACAGCCACTGCCATGCAATCA
  GAGATGTCCACATCAATTATGGAGACTACCACGACCCTGGCTACTAGCACAGCCAGAAGAGGAAAGCCCC
  CGACAAAAGAACCCATTAGCCAGACCACAGATGACATCCTTGTGGCCTCAGCAGAGTGTCCCAGCGATGA
  TGAGGACATTGACCCCTGTGAGCCGAGCTCAGGTGGGTTAGCCAACCCAACCCGAGCAGGCGGCAGAGAG
  CCGTATCCAGGCTCAGCAGAAGTGATCCGGGAGTCCAGCAGCACCACGGGTATGGTCGTTGGGATAGTAG
  CCGCTGCCGCCCTGTGCATCCTTATCCTCCTCTATGCCATGTACAAGTACAGAAACCGGGATGAAGGCTC
  ATACCATGTGGACGAGAGTCGAAACTACATCAGTAACTCAGCACAGTCCAATGGGGCTGTTGTAAAGGAG
  AAACAACCCAGCAGTGCGAAAAGCTCCAACAAAAATAAGAAAAACAAGGATAAAGAGTATTATGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215381 representing NM_138735
  Red=Cloning site Green=Tags(s)
  
  MYQRMLRCGAELGSPGGGGGGGGGGGAGGRLALLWIVPLTLSGLLGVAWGASSLGAHHIHHFHGSSKHHS
  VPIAIYRSPASLRGGHAGTTYIFSKGGGQITYKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLG
  DYLELHIHQGKIGVKFNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGRQLTI
  FNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEVPSSMTTESTATAMQS
  EMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECPSDDEDIDPCEPSSGGLANPTRAGGRE
  PYPGSAEVIRESSSTTGMVVGIVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKE
  KQPSSAKSSNKNKKNKDKEYYV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138735
• ORF Size: 1326 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138735.1, NM_138735.2, NM_138735.3, NM_138735.4, NP_620072.1
• RefSeq Size: 3712
• RefSeq ORF: 1329
• Locus ID: 9378
• Domains: LamG
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)
• MW: 42.1 kDa
• Gene Summary: This gene encodes a single-pass type I membrane protein that belongs to the neurexin family. Neurexins are cell-surface receptors that bind neuroligins to form Ca(2+)-dependent neurexin/neuroligin complexes at synapses in the central nervous system. This complex is required for efficient neurotransmission and is involved in the formation of synaptic contacts. Three members of this gene family have been studied in detail and are estimated to generate over 3,000 variants through the use of two alternative promoters (alpha and beta) and extensive alternative splicing in each family member. Recently, a third promoter (gamma) was identified for this gene in the 3' region. Mutations in this gene are associated with Pitt-Hopkins-like syndrome-2 and may contribute to susceptibility to schizophrenia. [provided by RefSeq, Aug 2016]