Fukutin (FKTN) (NM_006731) Human Tagged ORF Clone

CAT#: RC215400

FKTN (Myc-DDK-tagged)-Human fukutin (FKTN), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FKTN
• Synonyms: CMD1X; FCMD; LGMD2M; LGMDR13; MDDGA4; MDDGB4; MDDGC4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215400 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAGAATCAATAAGAACGTGGTTTTGGCCCTTTTAACGCTGACAAGTTCTGCATTTCTGCTGTTTC
  AGTTGTACTACTACAAGCACTATTTATCAACAAAGAATGGAGCTGGTTTGTCAAAATCCAAAGGAAGCCG
  AATTGGATTTGATAGCACACAGTGGCGTGCAGTTAAAAAATTTATTATGTTAACATCCAACCAAAATGTA
  CCAGTGTTTCTTATTGATCCTTTGATACTGGAATTGATTAATAAGAACTTTGAACAAGTCAAAAATACTT
  CTCATGGCTCTACTTCACAATGCAAGTTTTTCTGTGTTCCAAGAGACTTTACTGCATTTGCACTGCAGTA
  TCACCTATGGAAGAATGAGGAAGGCTGGTTTCGGATAGCTGAGAATATGGGATTTCAGTGCCTAAAGATT
  GAGAGTAAAGATCCCCGGCTAGACGGGATAGACTCACTCTCTGGAACTGAAATCCCCCTGCACTATATCT
  GCAAACTGGCCACTCATGCGATCCACTTGGTAGTCTTTCATGAGAGGAGTGGCAACTACCTCTGGCACGG
  CCACTTGAGACTTAAAGAACACATTGACAGGAAATTTGTTCCCTTCCGAAAGTTACAGTTTGGTCGTTAT
  CCAGGAGCTTTTGACAGGCCAGAGTTACAGCAAGTTACTGTTGATGGACTGGAAGTTCTCATTCCAAAGG
  ATCCAATGCACTTTGTAGAAGAAGTACCACACTCTAGGTTTATTGAGTGTAGGTATAAAGAAGCTCGAGC
  ATTCTTTCAGCAGTACCTTGATGATAACACTGTGGAAGCTGTGGCCTTTCGGAAGAGTGCAAAGGAATTA
  CTGCAACTAGCAGCGAAAACATTAAACAAATTGGGAGTACCATTCTGGCTGAGCAGTGGAACTTGTCTAG
  GATGGTATCGACAATGCAACATTATTCCTTATAGCAAAGATGTTGACCTAGGAATTTTTATACAAGATTA
  CAAATCTGATATTATTTTAGCATTTCAGGATGCAGGACTTCCGCTCAAACACAAATTTGGGAAGGTAGAA
  GACAGCTTGGAACTATCCTTCCAGGGAAAAGATGATGTAAAACTTGATGTTTTTTTCTTCTATGAAGAAA
  CTGATCACATGTGGAATGGAGGCACTCAGGCCAAAACAGGAAAAAAATTCAAATACCTGTTTCCGAAGTT
  TACACTGTGCTGGACTGAGTTTGTAGACATGAAGGTCCATGTACCCTGTGAAACCCTCGAATACATTGAA
  GCCAACTATGGTAAGACCTGGAAGATTCCTGTAAAGACGTGGGACTGGAAGCGCTCTCCTCCCAATGTGC
  AACCCAATGGAATCTGGCCTATTTCTGAGTGGGATGAGGTTATCCAGTTATAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215400 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSRINKNVVLALLTLTSSAFLLFQLYYYKHYLSTKNGAGLSKSKGSRIGFDSTQWRAVKKFIMLTSNQNV
  PVFLIDPLILELINKNFEQVKNTSHGSTSQCKFFCVPRDFTAFALQYHLWKNEEGWFRIAENMGFQCLKI
  ESKDPRLDGIDSLSGTEIPLHYICKLATHAIHLVVFHERSGNYLWHGHLRLKEHIDRKFVPFRKLQFGRY
  PGAFDRPELQQVTVDGLEVLIPKDPMHFVEEVPHSRFIECRYKEARAFFQQYLDDNTVEAVAFRKSAKEL
  LQLAAKTLNKLGVPFWLSSGTCLGWYRQCNIIPYSKDVDLGIFIQDYKSDIILAFQDAGLPLKHKFGKVE
  DSLELSFQGKDDVKLDVFFFYEETDHMWNGGTQAKTGKKFKYLFPKFTLCWTEFVDMKVHVPCETLEYIE
  ANYGKTWKIPVKTWDWKRSPPNVQPNGIWPISEWDEVIQLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006731
• ORF Size: 1383 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006731.1, NM_006731.2, NP_006722.1
• RefSeq Size: 7364 bp
• RefSeq ORF: 1386 bp
• Locus ID: 2218
• Cytogenetics: 9q31.2
• Protein Families: Transmembrane
• MW: 53.7 kDa
• Gene Summary: 'The protein encoded by this gene is a putative transmembrane protein that is localized to the cis-Golgi compartment, where it may be involved in the glycosylation of alpha-dystroglycan in skeletal muscle. The encoded protein is thought to be a glycosyltransferase and could play a role in brain development. Defects in this gene are a cause of Fukuyama-type congenital muscular dystrophy (FCMD), Walker-Warburg syndrome (WWS), limb-girdle muscular dystrophy type 2M (LGMD2M), and dilated cardiomyopathy type 1X (CMD1X). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2010]'