DAPP1 (NM_014395) Human Tagged ORF Clone
CAT#: RC215474
DAPP1 (Myc-DDK-tagged)-Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1)
"NM_014395" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | DAPP1 |
Synonyms | BAM32 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC215474 representing NM_014395
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGAGCAGAACTTCTAGAAGGGAAGATGAGCACCCAGGATCCCTCAGATCTGTGGAGCAGATCCG ATGGAGAGGCTGAGCTGCTCCAGGACTTGGGGTGGTATCACGGCAACCTCACACGCCATGCTGCTGAAGC TCTTCTCCTCTCAAATGGATGTGACGGCAGCTACCTTCTGAGGGACAGCAATGAGACCACCGGGCTGTAC TCTCTCTCTGTGAGGGCCAAAGATTCTGTTAAACACTTTCATGTTGAATATACTGGATATTCATTTAAAT TTGGCTTTAATGAATTCTCATCTTTGAAGGATTTTGTCAAGCATTTTGCAAATCAGCCTTTGATTGGAAG CGAGACAGGCACTCTGATGGTTCTAAAACATCCCTACCCAAGAAAAGTGGAAGAACCCTCCATTTATGAA TCTGTCCGGGTTCACACAGCAATGCAGACAGGAAGAACAGAAGATGACCTTGTGCCCACAGCACCTTCTC TGGGCACCAAAGAAGGTTACCTCACCAAACAGGGAGGCCTGGTCAAGACCTGGAAAACAAGATGGTTTAC TCTGCACAGGAATGAACTGAAATACTTCAAAGACCAGATGTCACCAGAACCAATTCGGATCCTAGACCTA ACAGAATGTTCAGCTGTACAATTCGATTATTCACAAGAAAGGGTAAACTGTTTTTGTTTGGTATTTCCAT TCAGGACATTTTATCTCTGTGCAAAGACCGGAGTAGAAGCTGATGAGTGGATCAAGATATTACGCTGGAA ATTGTCACAAATAAGAAAACAGCTCAACCAAGGGGAAGGCACGATCCGATCTCGGTCGTTCATCTTTAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC215474 representing NM_014395
Red=Cloning site Green=Tags(s) MGRAELLEGKMSTQDPSDLWSRSDGEAELLQDLGWYHGNLTRHAAEALLLSNGCDGSYLLRDSNETTGLY SLSVRAKDSVKHFHVEYTGYSFKFGFNEFSSLKDFVKHFANQPLIGSETGTLMVLKHPYPRKVEEPSIYE SVRVHTAMQTGRTEDDLVPTAPSLGTKEGYLTKQGGLVKTWKTRWFTLHRNELKYFKDQMSPEPIRILDL TECSAVQFDYSQERVNCFCLVFPFRTFYLCAKTGVEADEWIKILRWKLSQIRKQLNQGEGTIRSRSFIFK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_014395 |
ORF Size | 840 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014395.2, NP_055210.2 |
RefSeq Size | 2953 bp |
RefSeq ORF | 843 bp |
Locus ID | 27071 |
Domains | SH2, PH |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | B cell receptor signaling pathway |
MW | 32.6 kDa |
Gene Summary | May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K. [UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC108632 | DAPP1 (untagged)-Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1) |
USD 310.00 |
|
RG215474 | DAPP1 (GFP-tagged) - Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1) |
USD 460.00 |
|
RC215474L1 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), Myc-DDK-tagged |
USD 768.00 |
|
RC215474L2 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mGFP tagged |
USD 620.00 |
|
RC215474L3 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), Myc-DDK-tagged |
USD 620.00 |
|
RC215474L4 | Lenti ORF clone of Human dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mGFP tagged |
USD 620.00 |
{0} Product Review(s)
Be the first one to submit a review