LAMB4 (NM_007356) Human Tagged ORF Clone

CAT#: RC215486

LAMB4 (Myc-DDK-tagged)-Human laminin, beta 4 (LAMB4)

Product Images

Western blot validation of overexpression lysate (Cat# LY415985) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215486 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LAMB4
• Synonyms: laminin, beta 4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215486 representing NM_007356
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAATTTCAACTGACCCTTTTTTTGCACCTTGGGTGGCTCAGTTACTCAAAAGCTCAAGATGACTGCA
  ACAGGGGTGCCTGTCATCCCACCACTGGTGATCTCCTGGTGGGCAGGAACACGCAGCTTATGGCTTCTTC
  TACCTGTGGGCTGAGCAGAGCCCAGAAATACTGCATCCTCAGTTACCTGGAGGGGGAACAAAAATGCTCC
  ATCTGTGACTCTAGATTTCCATATGATCCGTATGACCAACCCAACAGCCACACCATTGAGAATGTCACTG
  TAAGTTTTGAACCAGACAGAGAAAAGAAATGGTGGCAATCTGAAAATGGTCTTGATCATGTCAGCATCAG
  ACTGGACTTAGAGGCATTATTTCGGTTCAGCCACCTTATCCTGACCTTTAAGACTTTTCGGCCTGCTGCA
  ATGTTAGTTGAACGTTCCACAGACTATGGACACAACTGGAAAGTGTTCAAATATTTTGCAAAAGACTGTG
  CCACTTCCTTTCCTAACATCACATCTGGCCAGGCCCAGGGAGTGGGAGACATTGTTTGTGACTCCAAATA
  CTCGGATATTGAACCCTCAACAGGTGGAGAGGTTGTTTTAAAAGTTTTGGATCCCAGTTTTGAAATTGAA
  AACCCTTATAGCCCCTACATCCAAGACCTTGTGACATTGACAAACCTGAGGATAAACTTTACCAAGCTCC
  ACACCCTTGGGGATGCTTTGCTTGGAAGGAGGCAAAATGATTCCCTTGATAAATACTACTATGCTCTGTA
  CGAGATGATTGTTCGGGGAAGCTGCTTTTGCAATGGCCATGCTAGCGAATGTCGCCCTATGCAGAAGATG
  CGGGGAGATGTTTTCAGCCCTCCTGGAATGGTTCACGGTCAGTGTGTGTGTCAGCACAATACAGATGGTC
  CGAACTGTGAGAGATGCAAGGACTTCTTCCAGGATGCTCCTTGGAGGCCAGCTGCAGACCTCCAGGACAA
  CGCTTGCAGATCGTGCAGCTGTAATAGCCACTCCAGCCGCTGTCACTTTGACATGACTACGTACCTGGCA
  AGCGGTGGCCTCAGCGGGGGCGTGTGTGAAGACTGCCAGCACAACACTGAGGGGCAGCACTGCGACCGCT
  GCAGACCCCTCTTCTACAGGGACCCGCTCAAGACCATCTCAGATCCCTACGCGTGCATTCCTTGTGAATG
  TGACCCCGATGGGACCATATCTGGTGGCATTTGTGTGAGCCACTCTGATCCTGCCTTAGGGTCTGTGGCC
  GGCCAGTGCCTTTGTAAAGAGAACGTGGAAGGAGCCAAATGCGACCAGTGCAAACCCAACCACTACGGAC
  TAAGCGCCACCGACCCCCTGGGCTGCCAGCCCTGCGACTGTAACCCCCTTGGGAGTCTGCCATTCTTGAC
  CTGTGATGTGGATACAGGCCAATGCTTGTGCCTGTCATATGTCACCGGAGCACACTGCGAAGAATGCACT
  GTTGGATACTGGGGCCTGGGAAATCATCTCCATGGGTGTTCTCCCTGTGACTGTGATATTGGAGGTGCTT
  ATTCTAACGTGTGCTCACCCAAGAATGGGCAGTGTGAATGCCGCCCACATGTCACTGGCCGTAGCTGCTC
  TGAACCAGCCCCTGGCTACTTCTTTGCTCCTTTGAATTTCTATCTCTACGAGGCAGAGGAAGCCACAACA
  CTCCAAGGACTGGCGCCTTTGGGCTCGGAGACGTTTGGCCAGAGTCCTGCTGTTCACGTTGTTTTAGGAG
  AGCCAGTTCCTGGGAACCCTGTTACATGGACTGGACCTGGATTTGCCAGGGTTCTCCCTGGGGCTGGCTT
  GAGATTTGCTGTCAACAACATTCCCTTTCCTGTGGACTTCACCATTGCCATTCACTATGAAACCCAGTCT
  GCAGCTGACTGGACTGTCCAGATTGTGGTGAACCCCCCTGGAGGGAGTGAGCACTGCATACCCAAGACTC
  TACAGTCAAAGCCTCAGTCTTTTGCCTTACCAGCGGCTACGAGAATCATGCTGCTTCCCACACCCATCTG
  TTTAGAACCAGATGTACAATATTCCATAGATGTCTATTTTTCTCAGCCTTTGCAAGGAGAGTCCCACGCT
  CATTCACATGTCCTGGTGGACTCTCTTGGCCTTATTCCCCAAATCAATTCATTGGAGAATTTCTGCAGCA
  AGCAGGACTTAGATGAGTATCAGCTTCACAACTGTGTTGAAATTGCCTCAGCAATGGGACCTCAAGTGCT
  CCCGGGTGCCTGTGAAAGGCTGATCATCAGCATGTCTGCCAAGCTGCATGATGGGGCTGTGGCCTGCAAG
  TGTCACCCCCAGGGCTCAGTCGGATCCAGCTGCAGCCGACTTGGAGGCCAGTGCCAGTGTAAACCTCTTG
  TGGTCGGGCGCTGCTGTGACAGGTGCTCAACTGGAAGCTATGATTTGGGGCATCACGGCTGTCACCCATG
  TCACTGCCATCCTCAAGGATCAAAGGACACTGTATGTGACCAAGTAACAGGACAGTGCCCCTGCCATGGA
  GAGGTGTCTGGCCGCCGCTGTGATCGCTGCCTGGCAGGCTACTTTGGATTTCCCAGCTGCCACCCTTGCC
  CTTGTAATAGGTTTGCTGAACTTTGTGATCCTGAGACAGGGTCATGCTTCAATTGTGGAGGCTTTACAAC
  TGGCAGAAACTGTGAAAGGTGTATTGATGGTTACTATGGAAATCCTTCTTCAGGACAGCCCTGTCGTCCT
  TGCCTGTGTCCAGATGATCCCTCAAGCAATCAGTATTTTGCCCATTCCTGTTATCAGAATCTGTGGAGCT
  CAGATGTAATCTGCAATTGTCTTCAAGGTTATACGGGTACTCAGTGTGGAGAATGCTCTACTGGTTTCTA
  TGGAAATCCAAGAATTTCAGGAGCACCTTGCCAACCATGTGCCTGCAACAACAACATAGATGTAACCGAT
  CCAGAGTCCTGCAGCCGGGTAACAGGGGAGTGCCTTCGATGTTTGCACAACACTCAGGGCGCAAACTGCC
  AGCTCTGCAAACCAGGTCACTATGGATCAGCCCTCAATCAGACCTGCAGAAGATGCTCCTGCCATGCTTC
  CGGCGTGAGTCCCATGGAGTGTCCCCCTGGTGGGGGAGCTTGCCTCTGTGACCCTGTCACTGGTGCATGT
  CCTTGTCTGCCGAATGTCACAGGCCTGGCCTGTGACCGTTGTGCTGATGGATACTGGAATCTGGTCCCTG
  GCAGAGGATGTCAGTCATGTGACTGTGACCCTAGGACCTCTCAAAGTAGCCACTGTGACCAGCTTACAGG
  CCAGTGTCCGTGTAAATTAGGTTACGGCGGGAAACGTTGCAGTGAGTGCCAGGAAAATTATTATGGTGAT
  CCACCTGGGCGATGCATTCCATGTGATTGTAACAGGGCAGGTACCCAGAAGCCCATCTGTGATCCAGACA
  CAGGCATGTGCCGCTGCCGGGAGGGTGTCAGCGGCCAGAGATGTGATCGCTGTGCCCGGGGACACAGCCA
  GGAATTCCCTACTTGTCTTCAATGTCACTTGTGCTTTGATCAGTGGGACCACACCATTTCTTCCCTCTCC
  AAAGCGGTGCAAGGGTTAATGAGACTGGCTGCTAACATGGAAGATAAAAGAGAGACCCTGCCTGTCTGTG
  AGGCAGACTTCAAAGACCTCAGAGGGAACGTGTCTGAAATAGAAAGGATTTTGAAACATCCTGTTTTCCC
  ATCTGGGAAATTCTTAAAAGTCAAGGATTATCATGACTCTGTTAGAAGACAAATCATGCAGCTAAATGAA
  CAACTGAAAGCAGTGTATGAATTTCAAGATCTGAAAGATACAATAGAAAGAGCAAAGAATGAAGCAGACC
  TCTTACTTGAAGACCTTCAGGAAGAAATTGATTTGCAATCCAGTGTCCTTAATGCAAGCATTGCGGACTC
  CTCAGAAAACATCAAGAAATATTATCACATATCATCATCTGCTGAAAAGAAAATTAATGAAACTAGTTCC
  ACCATTAATACCTCTGCAAATACAAGGAATGACTTACTTACCATCTTAGATACACTAACCTCAAAAGGAA
  ACTTGTCATTGGAAAGATTAAAGCAGATTAAGATACCAGATATCCAAATATTGAATGAAAAGGTGTGCGG
  AGATCCAGGAAATGTGCCATGTGTGCCCTTGCCCTGTGGCGGTGCTCTCTGCACGGGCCGGAAGGGGCAC
  AGGAAGTGTAGGGGTCCCGGCTGTCACGGCTCCCTGACCCTCTCAACGAATGCCCTCCAAAAAGCCCAGG
  AAGCAAAATCCATTATTCGTAATTTGGACAAACAGGTTCGTGGGTTGAAAAATCAGATCGAAAGTATAAG
  TGAACAGGCAGAAGTCTCCAAAAACAATGCCTTACAGCTGAGGGAAAAACTGGGAAATATAAGAAACCAA
  AGTGACTCTGAAGAAGAAAACATCAATCTTTTCATCAAAAAAGTGAAAAACTTTTTGTTAGAGGAAAACG
  TGCCTCCAGAAGACATCGAGAAGGTTGCGAATGGTGTGCTTGACATTCACCTACCAATTCCATCCCAAAA
  TCTAACCGATGAACTTGTCAAAATACAGAAACATATGCAACTCTGTGAGGATTACAGGACAGATGAAAAC
  AGGTTAAATGAAGAAGCAGATGGAGCCCAAAAGCTTTTGGTGAAGGCCAAAGCAGCTGAGAAAGCAGCAA
  ATATTCTATTAAATCTTGACAAAACATTGAACCAGTTACAACAAGCTCAAATCACTCAAGGACGGGCAAA
  CTCTACCATTACACAGCTGACTGCCAATATAACAAAAATAAAAAAGAATGTGCTGCAGGCTGAAAATCAA
  ACCAGGGAAATGAAGAGTGAGCTGGAGTTAGCAAAGCAGCGATCAGGGCTGGAGGATGGACTTTCCCTGC
  TGCAGACCAAGTTGCAAAGGCATCAAGACCACGCTGTCAATGCGAAAGTTCAGGCTGAATCTGCCCAACA
  CCAGGCTGGGAGTCTTGAGAAGGAATTTGTTGAGCTGAAAAAACAATATGCTATTCTCCAACGTAAGACA
  AGCACTACAGGACTAACAAAGGAGACATTAGGAAAAGTTAAACAGCTAAAAGATGCGGCAGAAAAATTGG
  CTGGAGATACAGAGGCCAAGATAAGAAGAATAACAGATTTAGAAAGGAAAATCCAAGATTTGAATCTAAG
  TAGACAAGCAAAAGCTGATCAACTGAGAATATTGGAAGATCAAGTTGTTGCCATTAAAAATGAAATTGTT
  GAACAAGAAAAAAAATATGCTAGGTGCTATAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215486 representing NM_007356
  Red=Cloning site Green=Tags(s)
  
  MQFQLTLFLHLGWLSYSKAQDDCNRGACHPTTGDLLVGRNTQLMASSTCGLSRAQKYCILSYLEGEQKCS
  ICDSRFPYDPYDQPNSHTIENVTVSFEPDREKKWWQSENGLDHVSIRLDLEALFRFSHLILTFKTFRPAA
  MLVERSTDYGHNWKVFKYFAKDCATSFPNITSGQAQGVGDIVCDSKYSDIEPSTGGEVVLKVLDPSFEIE
  NPYSPYIQDLVTLTNLRINFTKLHTLGDALLGRRQNDSLDKYYYALYEMIVRGSCFCNGHASECRPMQKM
  RGDVFSPPGMVHGQCVCQHNTDGPNCERCKDFFQDAPWRPAADLQDNACRSCSCNSHSSRCHFDMTTYLA
  SGGLSGGVCEDCQHNTEGQHCDRCRPLFYRDPLKTISDPYACIPCECDPDGTISGGICVSHSDPALGSVA
  GQCLCKENVEGAKCDQCKPNHYGLSATDPLGCQPCDCNPLGSLPFLTCDVDTGQCLCLSYVTGAHCEECT
  VGYWGLGNHLHGCSPCDCDIGGAYSNVCSPKNGQCECRPHVTGRSCSEPAPGYFFAPLNFYLYEAEEATT
  LQGLAPLGSETFGQSPAVHVVLGEPVPGNPVTWTGPGFARVLPGAGLRFAVNNIPFPVDFTIAIHYETQS
  AADWTVQIVVNPPGGSEHCIPKTLQSKPQSFALPAATRIMLLPTPICLEPDVQYSIDVYFSQPLQGESHA
  HSHVLVDSLGLIPQINSLENFCSKQDLDEYQLHNCVEIASAMGPQVLPGACERLIISMSAKLHDGAVACK
  CHPQGSVGSSCSRLGGQCQCKPLVVGRCCDRCSTGSYDLGHHGCHPCHCHPQGSKDTVCDQVTGQCPCHG
  EVSGRRCDRCLAGYFGFPSCHPCPCNRFAELCDPETGSCFNCGGFTTGRNCERCIDGYYGNPSSGQPCRP
  CLCPDDPSSNQYFAHSCYQNLWSSDVICNCLQGYTGTQCGECSTGFYGNPRISGAPCQPCACNNNIDVTD
  PESCSRVTGECLRCLHNTQGANCQLCKPGHYGSALNQTCRRCSCHASGVSPMECPPGGGACLCDPVTGAC
  PCLPNVTGLACDRCADGYWNLVPGRGCQSCDCDPRTSQSSHCDQLTGQCPCKLGYGGKRCSECQENYYGD
  PPGRCIPCDCNRAGTQKPICDPDTGMCRCREGVSGQRCDRCARGHSQEFPTCLQCHLCFDQWDHTISSLS
  KAVQGLMRLAANMEDKRETLPVCEADFKDLRGNVSEIERILKHPVFPSGKFLKVKDYHDSVRRQIMQLNE
  QLKAVYEFQDLKDTIERAKNEADLLLEDLQEEIDLQSSVLNASIADSSENIKKYYHISSSAEKKINETSS
  TINTSANTRNDLLTILDTLTSKGNLSLERLKQIKIPDIQILNEKVCGDPGNVPCVPLPCGGALCTGRKGH
  RKCRGPGCHGSLTLSTNALQKAQEAKSIIRNLDKQVRGLKNQIESISEQAEVSKNNALQLREKLGNIRNQ
  SDSEEENINLFIKKVKNFLLEENVPPEDIEKVANGVLDIHLPIPSQNLTDELVKIQKHMQLCEDYRTDEN
  RLNEEADGAQKLLVKAKAAEKAANILLNLDKTLNQLQQAQITQGRANSTITQLTANITKIKKNVLQAENQ
  TREMKSELELAKQRSGLEDGLSLLQTKLQRHQDHAVNAKVQAESAQHQAGSLEKEFVELKKQYAILQRKT
  STTGLTKETLGKVKQLKDAAEKLAGDTEAKIRRITDLERKIQDLNLSRQAKADQLRILEDQVVAIKNEIV
  EQEKKYARCYS
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007356
• ORF Size: 5283 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007356.1, NM_007356.2, NP_031382.1, NP_031382.2
• RefSeq Size: 5874
• RefSeq ORF: 5286
• Locus ID: 22798
• Protein Pathways: ECM-receptor interaction, Focal adhesion, Pathways in cancer, Small cell lung cancer
• MW: 193.4 kDa
• Gene Summary: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. [UniProtKB/Swiss-Prot Function]