ALG12 (NM_024105) Human Tagged ORF Clone

CAT#: RC215501

ALG12 (Myc-DDK-tagged)-Human asparagine-linked glycosylation 12, alpha-1,6-mannosyltransferase homolog (S. cerevisiae) (ALG12)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ALG12
• Synonyms: CDG1G; ECM39; hALG12; PP14673
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215501 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGGAAAGGGGTCATCAGGCAGGCGGCCCCTGCTGCTGGGGCTGCTGGTGGCCGTAGCCACTGTCC
  ACCTGGTCATCTGTCCCTACACCAAAGTGGAGGAGAGCTTCAACCTGCAGGCCACACATGACCTGCTCTA
  CCACTGGCAAGACCTGGAGCAGTACGACCATCTTGAGTTCCCCGGAGTCGTCCCCAGGACGTTCCTCGGG
  CCAGTGGTGATCGCAGTGTTCTCCAGCCCCGCGGTTTACGTGCTTTCGCTGTTAGAAATGTCCAAGTTTT
  ACTCTCAGCTAATAGTTAGAGGAGTGCTTGGACTCGGCGTGATTTTTGGACTCTGGACGTTACAAAAGGA
  AGTGAGACGGCACTTCGGGGCCATGGTGGCCACCATGTTCTGCTGGGTGACGGCCATGCAGTTCCACCTG
  ATGTTCTACTGCACGCGGACACTGCCCAATGTGCTGGCCCTGCCTGTAGTCCTGCTGGCCCTCGCGGCCT
  GGCTGCGGCACGAGTGGGCCCGCTTCATCTGGCTGTCAGCCTTCGCCATCATCGTGTTCAGGGTGGAGCT
  GTGCCTGTTCCTGGGCCTCCTGCTGCTGCTGGCCTTGGGCAACCGAAAGGTTTCTGTAGTCAGAGCCCTT
  CGCCACGCCGTCCCGGCAGGGATCCTCTGTTTAGGACTGACGGTTGCTGTGGACTCTTATTTTTGGCGGC
  AGCTCACTTGGCCGGAAGGAAAGGTGCTTTGGTACAACACTGTCCTGAACAAAAGCTCCAACTGGGGGAC
  CTCCCCGCTGCTGTGGTACTTCTACTCAGCCCTGCCCCGCGGCCTGGGCTGCAGCCTGCTCTTCATCCCC
  CTGGGCTTGGTAGACAGAAGGACGCACGCGCCGACGGTGCTGGCGCTGGGCTTCATGGCACTCTACTCCC
  TCCTGCCACACAAGGAGCTACGCTTCATCATCTATGCCTTCCCCATGCTCAACATCACGGCTGCCAGAGG
  CTGCTCCTACCTGCTGAATAACTATAAAAAGTCTTGGCTGTACAAAGCGGGGTCTCTGCTTGTGATCGGA
  CACCTCGTGGTGAATGCCGCCTACTCAGCCACGGCCCTGTATGTGTCCCATTTCAACTACCCAGGTGGCG
  TCGCAATGCAGAGGCTGCACCAGCTGGTGCCCCCCCAGACAGACGTCCTTCTGCACGTTGACGTGGCAGC
  CGCCCAGACAGGTGTGTCTCGGTTTCTCCAAGTCAACAGCGCCTGGAGGTACGACAAGAGGGAGGATGTG
  CAGCCGGGGACAGGCATGCTGGCATACACACACATCCTCATGGAGGCGGCCCCTGGGCTCCTGGCCCTCT
  ACAGGGACACACACCGGGTCCTGGCCAGCGTCGTGGGGACCACAGGTGTGAGTCTGAACCTGACCCAACT
  GCCCCCCTTCAACGTCCACCTGCAGACAAAGCTGGTGCTTCTGGAGAGGCTCCCCCGGCCGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215501 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGKGSSGRRPLLLGLLVAVATVHLVICPYTKVEESFNLQATHDLLYHWQDLEQYDHLEFPGVVPRTFLG
  PVVIAVFSSPAVYVLSLLEMSKFYSQLIVRGVLGLGVIFGLWTLQKEVRRHFGAMVATMFCWVTAMQFHL
  MFYCTRTLPNVLALPVVLLALAAWLRHEWARFIWLSAFAIIVFRVELCLFLGLLLLLALGNRKVSVVRAL
  RHAVPAGILCLGLTVAVDSYFWRQLTWPEGKVLWYNTVLNKSSNWGTSPLLWYFYSALPRGLGCSLLFIP
  LGLVDRRTHAPTVLALGFMALYSLLPHKELRFIIYAFPMLNITAARGCSYLLNNYKKSWLYKAGSLLVIG
  HLVVNAAYSATALYVSHFNYPGGVAMQRLHQLVPPQTDVLLHVDVAAAQTGVSRFLQVNSAWRYDKREDV
  QPGTGMLAYTHILMEAAPGLLALYRDTHRVLASVVGTTGVSLNLTQLPPFNVHLQTKLVLLERLPRPS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024105
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024105.1, NM_024105.2, NM_024105.3, NP_077010.1
• RefSeq Size: 2387 bp
• RefSeq ORF: 1467 bp
• Locus ID: 79087
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 54.6 kDa
• Gene Summary: This gene encodes a member of the glycosyltransferase 22 family. The encoded protein catalyzes the addition of the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man(7)GlcNAc(2)) required for protein glycosylation. Mutations in this gene have been associated with congenital disorder of glycosylation type Ig (CDG-Ig)characterized by abnormal N-glycosylation. [provided by RefSeq, Jul 2008]