MRVI1 (NM_130385) Human Tagged ORF Clone

CAT#: RC215536

MRVI1 (Myc-DDK-tagged)-Human murine retrovirus integration site 1 homolog (MRVI1), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MRVI1
• Synonyms: IRAG; JAW1L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215536 representing NM_130385
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAAAAGCTCCCCAGAGTGAAGAGAGGCTGGCCAGAGGAGGAAAGGAGAATAACTCAGTTTTAGCCT
  GTGGAGCCCAGGCCTCTTGGAGCATCTTTGGGGCTGACGCAGCGGAGGTTCCGGGCACACGTGGCCACTC
  CCAGCAGGAGGCTGCCATGCCCCACATTCCCGAGGACGAGGAGCCCCCCGGAGAGCCACAGGCAGCCCAG
  AGCCCTGCCGGCCAAGGTCCTCCTGCCGCAGGAGTATCTTGCAGTCCAACTCCCACGATTGTCCTGACTG
  GGGATGCCACTTCACCAGAAGGAGAAACCGACAAAAACCTGGCCAACAGAGTTCACAGTCCCCACAAGAG
  GCTTTCTCACCGACACTTGAAGGTGTCCACTGCCTCCCTGACATCTGTGGACCCCGCGGGGCACATCATT
  GACCTGGTGAATGACCAGCTGCCAGACATCAGCATCTCAGAGGAGGACAAGAAGAAAAACCTGGCGCTGC
  TGGAAGAAGCCAAGTTGGTGAGTGAGCGATTCCTGACCCGCCGTGGGAGGAAGTCCAGGAGCAGCCCCGG
  AGACTCCCCATCAGCTGTTTCCCCGAACCTCAGCCCCAGCGCTTCTCCTACATCCTCTCGGAGCAACTCA
  CTTACAGTCCCCACCCCGCCAGGTTTGGATGTGTGCAGTGGCCCGCCATCCCCTCTGCCTGGAGCACCAC
  CACAGAAGGGGGATGAGGCCGACGTCTCTTCACCTCACCCTGGCGAGCCTAACGTCCCCAAAGGGCTAGC
  TGACAGGAAGCAGAATGACCAGAGGAAAGTGTCTCAGGGCAGGCTGGCTCCTCGTCCTCCTCCAGTTGAG
  AAGTCCAAAGAGATTGCAATAGAACAAAAGGAAAACTTCGATCCCCTCCAGTACCCCGAGACCACACCCA
  AAGGCCTAGCTCCTGTTACAAACAGCAGTGGGAAAATGGCCCTGAACAGCCCTCAGCCTGGCCCCGTGGA
  GAGCGAGCTGGGGAAGCAGCTCTTGAAAACGGGCTGGGAGGGCAGCCCTCTGCCGAGAAGTCCAACCCAG
  GATGCGGCAGGAGTGGGTCCCCCAGCCTCCCAGGGGAGAGGCCCAGCTGGAGAGCCGATGGGGCCCGAGG
  CTGGCTCCAAAGCTGAGCTTCCACCCACTGTGTCCCGGCCCCCGCTGCTGCGAGGGCTCTCCTGGGACAG
  TGGCCCTGAAGAACCTGGCCCCCGGCTGCAGAAAGTGCTTGCCAAGCTGCCACTGGCAGAGGAAGAAAAG
  CGTTTTGCAGGCAAGGCCGGCGGCAAGCTGGCCAAGGCCCCTGGTCTCAAAGACTTTCAGATACAAGTGC
  AGCCCGTGCGGATGCAGAAACTGACCAAGCTCCGAGAGGAGCACATCCTGATGAGAAATCAGAACTTAGT
  GGGGCTCAAGCTTCCAGACCTTAGTGAAGCAGCTGAGCAGGAAAAAGGGCTTCCTTCTGAACTCTCCCCA
  GCTATTGAGGAAGAAGAGTCAAAGAGTGGCTTAGATGTCATGCCTAATATTTCTGATGTGCTGCTGCGCA
  AACTGCGGGTCCACAGGAGTCTCCCTGGAAGTGCCCCTCCACTCACTGAAAAGGAAGTTGAGAACGTGTT
  TGTGCAACTGTCCTTGGCCTTTAGAAATGACAGCTACACTCTGGAATCTAGAATTAACCAGGCTGAAAGG
  GAACGCAACCTGACAGAGGAGAACACTGAGAAAGAACTGGAAAACTTCAAAGCTTCCATTACGTCCTCAG
  CTTCACTCTGGCACCACTGTGAGCACCGGGAAACCTACCAGAAGTTGCTGGAGGACATCGCTGTCCTGCA
  CCGCCTGGCTGCCCGCCTCTCCAGCCGAGCTGAGGTGGTAGGCGCCGTCCGCCAGGAAAAGCGCATGTCG
  AAAGCAACGGAAGTGATGATGCAGTATGTGGAGAATCTAAAGAGGACGTATGAGAAGGACCATGCGGAGC
  TCATGGAGTTTAAAAAGCTTGCAAATCAGAATTCAAGCCGCAGCTGTGGCCCCTCTGAAGATGGGGTCCC
  TCGCACGGCACGGTCCATGTCCCTCACGCTGGGAAAGAATATGCCTCGCCGGAGGGTCAGCGTTGCTGTG
  GTTCCTAAGTTTAATGCCCTGAATCTGCCTGGCCAAACTCCCAGCTCATCATCCATTCCCTCCTTACCAG
  CCTTGTCGGAATCACCCAATGGGAAAGGCAGCCTACCTGTCACTTCAGCACTGCCTGCACTTTTGGAAAA
  TGGAAAGACAAATGGGGACCCAGATTGTGAAGCCTCTGCTCCTGCGCTGACCCTGAGCTGCCTGGAGGAG
  CTTAGTCAGGAGACCAAGGCCAGGATGGAGGAAGAAGCCTACAGCAAGGGATTCCAAGAAGGTCTAAAGA
  AGACCAAAGAACTTCAAGACCTGAAGGAGGAGGAGGAAGAACAGAAGAGTGAGAGTCCTGAGGAACCTGA
  AGAGGTAGAAGAAACTGAGGAAGAGGAAAAGGGCCCAAGAAGCAGCAAACTTGAAGAATTGGTCCATTTC
  TTACAAGTCATGTATCCCAAACTGTGTCAGCACTGGCAAGTGATCTGGATGATGGCTGCAGTGATGCTGG
  TCTTGACTGTTGTGCTGGGGCTCTACAATTCCTATAACTCTTGTGCAGAGCAGGCTGATGGGCCCCTTGG
  AAGATCCACTTGCTCGGCAGCCCAGAGGGACTCCTGGTGGAGCTCAGGACTCCAGCATGAGCAGCCTACA
  GAGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215536 representing NM_130385
  Red=Cloning site Green=Tags(s)
  
  MVKAPQSEERLARGGKENNSVLACGAQASWSIFGADAAEVPGTRGHSQQEAAMPHIPEDEEPPGEPQAAQ
  SPAGQGPPAAGVSCSPTPTIVLTGDATSPEGETDKNLANRVHSPHKRLSHRHLKVSTASLTSVDPAGHII
  DLVNDQLPDISISEEDKKKNLALLEEAKLVSERFLTRRGRKSRSSPGDSPSAVSPNLSPSASPTSSRSNS
  LTVPTPPGLDVCSGPPSPLPGAPPQKGDEADVSSPHPGEPNVPKGLADRKQNDQRKVSQGRLAPRPPPVE
  KSKEIAIEQKENFDPLQYPETTPKGLAPVTNSSGKMALNSPQPGPVESELGKQLLKTGWEGSPLPRSPTQ
  DAAGVGPPASQGRGPAGEPMGPEAGSKAELPPTVSRPPLLRGLSWDSGPEEPGPRLQKVLAKLPLAEEEK
  RFAGKAGGKLAKAPGLKDFQIQVQPVRMQKLTKLREEHILMRNQNLVGLKLPDLSEAAEQEKGLPSELSP
  AIEEEESKSGLDVMPNISDVLLRKLRVHRSLPGSAPPLTEKEVENVFVQLSLAFRNDSYTLESRINQAER
  ERNLTEENTEKELENFKASITSSASLWHHCEHRETYQKLLEDIAVLHRLAARLSSRAEVVGAVRQEKRMS
  KATEVMMQYVENLKRTYEKDHAELMEFKKLANQNSSRSCGPSEDGVPRTARSMSLTLGKNMPRRRVSVAV
  VPKFNALNLPGQTPSSSSIPSLPALSESPNGKGSLPVTSALPALLENGKTNGDPDCEASAPALTLSCLEE
  LSQETKARMEEEAYSKGFQEGLKKTKELQDLKEEEEEQKSESPEEPEEVEETEEEEKGPRSSKLEELVHF
  LQVMYPKLCQHWQVIWMMAAVMLVLTVVLGLYNSYNSCAEQADGPLGRSTCSAAQRDSWWSSGLQHEQPT
  EQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130385
• ORF Size: 2736 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_130385.3, NP_569056.4
• RefSeq Size: 6376
• RefSeq ORF: 2739
• Locus ID: 10335
• Protein Families: Transmembrane
• Protein Pathways: Vascular smooth muscle contraction
• MW: 98.9 kDa
• Gene Summary: This gene is similar to a putative mouse tumor suppressor gene (Mrvi1) that is frequently disrupted by mouse AIDS-related virus (MRV). The encoded protein, which is found in the membrane of the endoplasmic reticulum, is similar to Jaw1, a lymphoid-restricted protein whose expression is down-regulated during lymphoid differentiation. This protein is a substrate of cGMP-dependent kinase-1 (PKG1) that can function as a regulator of IP3-induced calcium release. Studies in mouse suggest that MRV integration at Mrvi1 induces myeloid leukemia by altering the expression of a gene important for myeloid cell growth and/or differentiation, and thus this gene may function as a myeloid leukemia tumor suppressor gene. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene, and alternative translation start sites, including a non-AUG (CUG) start site, are used. [provided by RefSeq, May 2011]