CDCP1 (NM_178181) Human Tagged ORF Clone

CAT#: RC215553

CDCP1 (Myc-DDK-tagged)-Human CUB domain containing protein 1 (CDCP1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406018) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215553 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDCP1
• Synonyms: CD318; SIMA135; TRASK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215553 representing NM_178181
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCCTGAACTGCGGGGTCTCTATCGCACTGCTAGGGGTTCTGCTGCTGGGTGCGGCGCGCCTGC
  CGCGCGGGGCAGAAGCTTTTGAGATTGCTCTGCCACGAGAAAGCAACATTACAGTTCTCATAAAGCTGGG
  GACCCCGACTCTGCTGGCAAAACCCTGTTACATCGTCATTTCTAAAAGACATATAACCATGTTGTCCATC
  AAGTCTGGAGAAAGAATAGTCTTTACCTTTAGCTGCCAGAGTCCTGAGAATCACTTTGTCATAGAGATCC
  AGAAAAATATTGACTGTATGTCAGGCCCATGTCCTTTTGGGGAGGTTCAGCTTCAGCCCTCGACATCGTT
  GTTGCCTACCCTCAACAGAACTTTCATCTGGGATGTCAAAGCTCATAAGAGCATCGGTTTAGAGCTGCAG
  TTTTCCATCCCTCGCCTGAGGCAGATCGGTCCGGGTGAGAGCTGCCCAGACGGAGTCACTCACTCCATCA
  GCGGCCGAATCGATGCCACCGTGGTCAGGATCGGAACCTTCTGCAGCAATGGCACTGTGTCCCGGATCAA
  GATGCAAGAAGGAGTGAAAATGGCCTTACACCTCCCATGGTTCCACCCCAGAAATGTCTCCGGCTTCAGC
  ATTGCAAACCGCTCATCTATAAAACGTCTGTGCATCATCGAGTCTGTGTTTGAGGGTGAAGGCTCAGCAA
  CCCTGATGTCTGCCAACTACCCAGAAGGCTTCCCTGAGGATGAGCTCATGACGTGGCAGTTTGTCGTTCC
  TGCACACCTGCGGGCCAGCGTCTCCTTCCTCAACTTCAACCTCTCCAACTGTGAGAGGAAGGAGGAGCGG
  GTTGAATACTACATCCCGGGCTCCACCACCAACCCCGAGGTGTTCAAGCTGGAGGACAAGCAGCCTGGGA
  ACATGGCGGGGAACTTCAACCTCTCTCTGCAAGGCTGTGACCAAGATGCCCAAAGTCCAGGGATCCTCCG
  GCTGCAGTTCCAAGTTTTGGTCCAACATCCACAAAATGAAAGCAGTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215553 representing NM_178181
  Red=Cloning site Green=Tags(s)
  
  MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSI
  KSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQ
  FSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFS
  IANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEER
  VEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178181
• ORF Size: 1029 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178181.1, NM_178181.2, NP_835488.1
• RefSeq Size: 1410 bp
• RefSeq ORF: 1032 bp
• Locus ID: 64866
• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• MW: 37.6 kDa
• Gene Summary: This gene encodes a transmembrane protein which contains three extracellular CUB domains and acts as a substrate for Src family kinases. The protein plays a role in the tyrosine phosphorylation-dependent regulation of cellular events that are involved in tumor invasion and metastasis. Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]