GRK4 (NM_001004057) Human Tagged ORF Clone

CAT#: RC215579

GRK4 (Myc-DDK-tagged)-Human G protein-coupled receptor kinase 4 (GRK4), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRK4
• Synonyms: GPRK2L; GPRK4; GRK4a; IT11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215579 representing NM_001004057
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCGAGAACATCGTGGCCAACTCGCTGCTGCTGAAAGCGCGTCAAGGAGGATATGGCAAAAAAA
  GTGGTCGTAGTAAAAAATGGAAGGAGATACTGACACTGCCTCCTGTCAGCCAGTGCAGTGAGCTTAGACA
  TTCCATTGAAAAGGATTATAGCAGTCTTTGTGACAAGCAACCGATAGGAAGACGTCTCTTCAGGCAGTTC
  TGTGATACCAAACCCACTCTAAAGAGGCACATTGAATTCTTGGATGCAGTGGCAGAATATGAAGTTGCCG
  ATGATGAGGACCGAAGTGATTGTGGACTGTCAATCTTAGATAGATTCTTCAATGATAAGTTGGCAGCCCC
  TTTACCAGAAATACCTCCAGATGTTGTGACAGAATGTAGATTGGGACTGAAGGAGGAGAACCCTTCCAAA
  AAAGCCTTTGAGGAATGTACTAGAGTTGCCCATAACTACCTAAGAGGGGAACCATTTGAAGAATACCAAG
  AAAGCTCATATTTTTCTCAGTTTTTACAATGGAAATGGCTGGAAAGGCAACCCGTAACAAAGAACACATT
  TAGACATTACAGAGTTCTAGGAAAAGGCGGATTTGGAGAGGTTTGCGCCTGTCAAGTGCGAGCCACAGGA
  AAAATGTATGCCTGCAAAAAGCTACAAAAAAAAAGAATAAAGAAGAGGAAAGGTGAAGCTATGGCTCTAA
  ATGAGAAAAGAATTCTGGAGAAAGTGCAAAGTAGATTCGTAGTTAGTTTAGCCTACGCTTATGAAACCAA
  AGATGCCTTGTGCTTGGTGCTCACCATTATGAATGGAGGGGATTTGAAGTTTCACATTTACAACCTGGGC
  AATCCCGGCTTTGATGAGCAGAGAGCCGTTTTCTATGCTGCAGAGCTGTGTTGCGGCTTGGAAGATTTAC
  AGAGGGAAAGAATTGTATACAGAGACTTGAAGCCTGAGAATATTCTCCTTGATGATCGTGGACACATCCG
  GATTTCAGACCTCGGTTTGGCCACAGAGATCCCAGAAGGACAGAGGGTTCGAGGAAGAGTTGGAACAGTC
  GGCTACATGGCACCTGAAGTTGTCAATAATGAAAAGTATACGTTTAGTCCCGATTGGTGGGGACTTGGCT
  GTCTGATCTATGAAATGATTCAGGGACATTCTCCATTCAAAAAATACAAAGAGAAAGTCAAATGGGAGGA
  GGTCGATCAAAGAATCAAGAATGATACCGAGGAGTATTCTGAGAAGTTTTCAGAGGATGCCAAATCTATC
  TGCAGGATGTTACTCACCAAGAATCCAAGCAAGCGGCTGGGCTGCAGGGGCGAGGGAGCGGCTGGGGTGA
  AGCAGCACCCCGTGTTCAAGGACATCAACTTCAGGAGGCTGGAGGCAAACATGCTGGAGCCCCCTTTCTG
  TCCTGATCCTCATGCCGTTTACTGTAAGGACGTCCTGGATATCGAGCAGTTCTCGGCGGTGAAAGGGATC
  TACCTGGACACCGCAGATGAAGACTTCTATGCTCGGTTTGCTACCGGGTGTGTCTCCATCCCCTGGCAGA
  ATGAGGGCTGCCTGACCATGGTCCCCAGTGAGAAGGAAGTGGAACCCAAGCAATGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215579 representing NM_001004057
  Red=Cloning site Green=Tags(s)
  
  MELENIVANSLLLKARQGGYGKKSGRSKKWKEILTLPPVSQCSELRHSIEKDYSSLCDKQPIGRRLFRQF
  CDTKPTLKRHIEFLDAVAEYEVADDEDRSDCGLSILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSK
  KAFEECTRVAHNYLRGEPFEEYQESSYFSQFLQWKWLERQPVTKNTFRHYRVLGKGGFGEVCACQVRATG
  KMYACKKLQKKRIKKRKGEAMALNEKRILEKVQSRFVVSLAYAYETKDALCLVLTIMNGGDLKFHIYNLG
  NPGFDEQRAVFYAAELCCGLEDLQRERIVYRDLKPENILLDDRGHIRISDLGLATEIPEGQRVRGRVGTV
  GYMAPEVVNNEKYTFSPDWWGLGCLIYEMIQGHSPFKKYKEKVKWEEVDQRIKNDTEEYSEKFSEDAKSI
  CRMLLTKNPSKRLGCRGEGAAGVKQHPVFKDINFRRLEANMLEPPFCPDPHAVYCKDVLDIEQFSAVKGI
  YLDTADEDFYARFATGCVSIPWQNEGCLTMVPSEKEVEPKQC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004057
• ORF Size: 1596 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004057.1, NP_001004057.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 1599 bp
• Locus ID: 2868
• Cytogenetics: 4p16.3
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis
• MW: 61 kDa
• Gene Summary: 'This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]'