PTCRA (NM_138296) Human Tagged ORF Clone

CAT#: RC215794

PTCRA (Myc-DDK-tagged)-Human pre T-cell antigen receptor alpha (PTCRA)

Product Images

Western blot validation of overexpression lysate (Cat# LY408715) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215794 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PTCRA
• Synonyms: PT-ALPHA; PTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215794 representing NM_138296
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGTACATGGCTGCTACTTCTCCTGGCCCTTGGGTGTCCAGCCCTACCCACAGGTGTGGGCGGCA
  CACCCTTTCCTTCTCTGGCCCCACCAATCATGCTGCTGGTGGATGGAAAGCAGCAGATGGTGGTGGTCTG
  CCTGGTCCTTGATGTTGCACCCCCTGGCCTTGACAGCCCCATCTGGTTCTCAGCCGGCAATGGCAGTGCA
  CTGGATGCCTTCACCTATGGCCCTTCCCCAGCAACGGATGGCACCTGGACCAACTTGGCCCATCTCTCCC
  TGCCTTCTGAGGAGCTGGCATCCTGGGAGCCTTTGGTCTGCCACACTGGGCCTGGGGCTGAGGGTCACAG
  CAGGAGTACACAGCCCATGCATCTGTCAGGAGAGGCTTCTACAGCCAGGACCTGCCCCCAGGAGCCTCTC
  AGGGGGACACCGGGTGGGGCGCTGTGGCTGGGGGTCCTGCGGCTGCTGCTCTTCAAGCTGCTGCTGTTTG
  ACCTGCTCCTGACCTGCAGCTGCCTGTGCGACCCCGCGGGCCCGCTGCCTTCCCCCGCAACCACCACCCG
  CCTGCGAGCCCTCGGCTCCCATCGACTGCACCCGGCCACGGAGACTGGGGGACGAGAGGCCACCAGCTCA
  CCCAGACCCCAGCCTCGGGACCGCCGCTGGGGTGACACCCCTCCGGGTCGGAAGCCCGGGAGCCCAGTAT
  GGGGGGAAGGGTCTTACCTCAGCAGTTACCCCACTTGCCCAGCACAGGCCTGGTGCTCAAGATCTGCCCT
  CAGGGCTCCTTCCTCCAGTCTTGGAGCATTTTTTGCAGGTGACCTGCCTCCTCCTCTGCAGGCTGGAGCT
  GCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215794 representing NM_138296
  Red=Cloning site Green=Tags(s)
  
  MAGTWLLLLLALGCPALPTGVGGTPFPSLAPPIMLLVDGKQQMVVVCLVLDVAPPGLDSPIWFSAGNGSA
  LDAFTYGPSPATDGTWTNLAHLSLPSEELASWEPLVCHTGPGAEGHSRSTQPMHLSGEASTARTCPQEPL
  RGTPGGALWLGVLRLLLFKLLLFDLLLTCSCLCDPAGPLPSPATTTRLRALGSHRLHPATETGGREATSS
  PRPQPRDRRWGDTPPGRKPGSPVWGEGSYLSSYPTCPAQAWCSRSALRAPSSSLGAFFAGDLPPPLQAGA
  A
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_138296
• ORF Size: 843 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_138296.1, NM_138296.2, NP_612153.1
• RefSeq Size: 1080
• RefSeq ORF: 846
• Locus ID: 171558
• Protein Families: Transmembrane
• Protein Pathways: Notch signaling pathway
• MW: 29.1 kDa
• Gene Summary: The protein encoded by this gene is a single-pass type I membrane protein that is found in immmature but not mature T-cells. Along with TCRB and CD3 complex, the encoded protein forms the pre-T-cell receptor complex, which regulates early T-cell development. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]